Title:
OPTIMIZED CRISPR/SPCAS12F1 SYSTEM, ENGINEERED GUIDE RNA AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/206871
Kind Code:
A1
Abstract:
Provided are an optimized CRISPR/SpCas12f1 system, an engineered guide RNA and use thereof. The CRISPR/SpCas12f1 genome editing system comprises: an expression construct comprising an SpCas12f1 nuclease; and an expression construct comprising an expressed DNA sequence for a guide RNA corresponding to the SpCas12f1 nuclease and a targeting sequence of a target sequence. Also provided is a method for optimizing a guide RNA corresponding to an SpCas12f1 nuclease, comprising: modifying tracrRNA and crRNA individually or in combination. By using the gene editing system of the present invention, a target gene in a cell can be accurately edited; by using the optimized guide RNA of the present invention, the editing efficiency and applicability of the editing system can be greatly improved.
Inventors:
JI QUANJIANG (CN)
WANG YUJUE (CN)
WU ZHAOWEI (CN)
WANG YUJUE (CN)
WU ZHAOWEI (CN)
Application Number:
PCT/CN2022/113354
Publication Date:
November 02, 2023
Filing Date:
August 18, 2022
Export Citation:
Assignee:
UNIV SHANGHAI TECH (CN)
International Classes:
C12N15/52; C12N9/22; C12N15/113
Domestic Patent References:
| WO2020098772A1 | 2020-05-22 | |||
| WO2021238128A1 | 2021-12-02 | |||
| WO2020088450A1 | 2020-05-07 | |||
| WO2019126762A2 | 2019-06-27 |
Foreign References:
| CN113166744A | 2021-07-23 |
Other References:
GRETA BIGELYTE ET AL.: "Miniature Type V-F CRISPR-Cas Nucleases Enable Targeted DNA Modification in Cells", NATURE COMMUNICATIONS, vol. 12, no. 1, 26 October 2021 (2021-10-26), XP093104321, ISSN: 2041-1723, DOI: 10.1038/s41467-021-26469-4
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (CN)
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