Title:
P16 EXPRESSION CONSTRUCTS AND THEIR APPLICATION IN CANCER THERAPY
Document Type and Number:
WIPO Patent Application WO1997003635
Kind Code:
A3
Abstract:
A variety of genetic constructs are disclosed that will find both in vitro and in vivo use in the area of tumor biology and cancer therapy. In particular, expression constructs are provided that contain a p16 encoding region and other regulatory elements necessary for the expression of a p16 transcript. One version of the expression construct is a replication-deficient adenoviral vector. Also provided are methods for the transformation of cell lines and the inhibition of cancer cell proliferation.
Inventors:
JIN XIAOMEI
ROTH JACK
ROTH JACK
Application Number:
PCT/US1996/011787
Publication Date:
May 15, 1997
Filing Date:
July 17, 1996
Export Citation:
Assignee:
UNIV TEXAS (US)
International Classes:
C12N15/09; A61K48/00; A61P35/00; C07K14/47; C12N5/10; C12N15/00; C12Q1/68; A61K38/00; C12R1/92; (IPC1-7): A61K/
Domestic Patent References:
| WO1996025507A2 | 1996-08-22 | |||
| WO1995028169A1 | 1995-10-26 |
Other References:
JIN X. ET AL.: "Cell cycle arrest and inhibition of tumor cell proliferation by the p16ink4 gene mediated by an adenovirus vector", CANCER RESEARCH, vol. 55, no. 15, 1 August 1995 (1995-08-01), pages 3250 - 3253, XP002026156
OKAMOTO A. ET AL.: "Mutations and altered expression of p16ink4 in human cancer", PROC.NATL.ACAD.SCI.USA, vol. 91, November 1994 (1994-11-01), pages 11045 - 11049, XP002026157
ARAP W. ET AL.: "Replacement of the p16/CDKN2 gene suppresses human glioma cell growth", CANCER RESEARCH, vol. 55, 15 March 1995 (1995-03-15), pages 1351 - 1354, XP002026158
ZHANG W.-W. ET AL.: "High-efficiency gene transfer and high-level expression of wild-type p53 in human lung cancer cells mediated by recombinant adenovirus", CANCER GENE THERAPY, vol. 1, no. 1, 1994, pages 5 - 13, XP000579621
SERRANO M. ET AL.: "A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4", NATURE, vol. 366, 1993, pages 704 - 707, XP002008795
OKAMOTO A. ET AL.: "Mutations and altered expression of p16ink4 in human cancer", PROC.NATL.ACAD.SCI.USA, vol. 91, November 1994 (1994-11-01), pages 11045 - 11049, XP002026157
ARAP W. ET AL.: "Replacement of the p16/CDKN2 gene suppresses human glioma cell growth", CANCER RESEARCH, vol. 55, 15 March 1995 (1995-03-15), pages 1351 - 1354, XP002026158
ZHANG W.-W. ET AL.: "High-efficiency gene transfer and high-level expression of wild-type p53 in human lung cancer cells mediated by recombinant adenovirus", CANCER GENE THERAPY, vol. 1, no. 1, 1994, pages 5 - 13, XP000579621
SERRANO M. ET AL.: "A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4", NATURE, vol. 366, 1993, pages 704 - 707, XP002008795
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