Title:
P43 ANTIGEN FOR THE IMMUNODIAGNOSIS OF CANINE EHRLICHIOSIS AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2001082862
Kind Code:
A3
Abstract:
Canine monocytic ehrlichiosis, caused by Ehrlichia canis is a potentially fatal disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. In the invention described herein, a new immunoreactive E. canis surface protein gene of 1170-bp was cloned, which encodes a protein with a predicted molecular mass of 42.6 kilodaltons (P43). The P43 gene was not found in E. chaffeensis DNA by Southern blot, and antisera against recombinant P43 (rP43) did not react with E. chaffeensis by IFA. The P43 was located on the surface of E. canis by immunoelectron microscopy. Forty-two dogs exhibiting signs and/or hematologic abnormalities associated with canine ehrlichiosis were tested by IFA and by Western immunoblot. Among the 22 samples that were IFA positive for E. canis, 100 % reacted with the rP43, 96 % with the rP28, and 96 % with the rP140. The specificity of the recombinant proteins compared to IFA was 96 % for rp28, 88 % for P43 and 63 % for P140. Results of this study demonstrate that the rP43 and rP28 are sensitive and reliable serodiagnostic antigens for the diagnosis of Ehrlichia canis infections.
Inventors:
WALKER DAVID H
MCBRIDE JERE W
MCBRIDE JERE W
Application Number:
PCT/US2001/013446
Publication Date:
April 04, 2002
Filing Date:
April 27, 2001
Export Citation:
Assignee:
RES DEV FOUNDATION (US)
International Classes:
C07K14/29; A61K38/00; (IPC1-7): A61K38/00; A61K39/00; A61K39/002; A61K39/38; A61K39/40; A61K39/395; A61K49/00; C12P19/34; C12P21/04; C12P21/06; C07K1/00; C07K14/00
Foreign References:
| US6043085A | 2000-03-28 |
Other References:
MCBRIDE J.W. ET AL.: "Molecular cloning of the gene for a conserved major immunoreactive 28-kilodalton protein of ehrlichia canis: a potential serodiagnostic antigen", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 6, no. 3, May 1999 (1999-05-01), pages 392 - 399, XP002905766
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