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Title:
PARTICLE ANALYSIS DEVICE, PARTICLE ANALYSIS DEVICE PROGRAM, AND PARTICLE ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2023/190534
Kind Code:
A1
Abstract:
A particle analysis device 100 which, in order to ensure sufficient observation time using a PTA method and without being affected by the fading of particles having fluorescent markers or autofluorescence properties, can operate in a fluorescence observation mode in which fluorescent markers added to particles or fluorescence emitted by the particles themselves are imaged by irradiating the particles with excitation light, and a scattered light observation mode in which scattered light produced by irradiating the particles with light is imaged, said particle analysis device comprising: a particle identification unit 41 that identifies particles to which fluorescent markers have been added or particles emitting fluorescence from fluorescence image data obtained in the fluorescence observation mode; and an analysis unit 42 that analyzes the properties of the particles by finding the diffusion speed due to Brownian motion of the particles identified by the particle identification unit 41 from scattered light image data obtained in the scattered light observation mode, which has a higher frame rate than the fluorescence observation mode.

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Inventors:
SUGASAWA HIROSUKE (JP)
TATEWAKI YASUHIRO (JP)
IGUSHI TATSUO (JP)
Application Number:
PCT/JP2023/012555
Publication Date:
October 05, 2023
Filing Date:
March 28, 2023
Export Citation:
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Assignee:
HORIBA LTD (JP)
International Classes:
G01N15/02; C12M1/00; C12M1/34; G01N21/64
Foreign References:
US20190277743A12019-09-12
JP2012198058A2012-10-18
JP2009014490A2009-01-22
US20170102329A12017-04-13
US6459093B12002-10-01
Other References:
AARON M. STREETS, SOURIGUES YANNICK, KOPITO RON, MELKI RONALD, QUAKE STEPHEN, GEORGAKOUDI IRENE: "Simultaneous Measurement of Amyloid Fibril Formation by Dynamic Light Scattering and Fluorescence Reveals Complex Aggregation Kinetics", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 8, no. 1, 17 January 2013 (2013-01-17), pages e54541, XP055212457, DOI: 10.1371/journal.pone.0054541
Attorney, Agent or Firm:
NISHIMURA, Ryuhei et al. (JP)
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