Title:
PATIENT-MATCHED ORGANOID SYSTEMS FOR STUDYING CANCER
Document Type and Number:
WIPO Patent Application WO/2021/081491
Kind Code:
A3
Abstract:
In certain example embodiments, the invention provides a method of generating an ex vivo cell-based system comprising dissociating an original tissue sample obtained from a subject into a single cell population; determining an in vivo phenotype of the tissue sample by conducting single-cell RNA analysis on a first portion of the single cells; establishing an ex vivo cell-based system from a second portion of the single cells; and culturing the ex vivo cell-based system in a medium or conditions selected to maintain the in vivo phenotype. In some embodiments, the original tissue sample is a tumor tissue sample, such as a pancreatic ductal adenocarcinoma (PDAC) tumor sample.
Inventors:
SHALEK ALEXANDER (US)
WINTER PETER (US)
NAVIA ANDREW (US)
RAGHAVAN SRIVATSAN (US)
HAHN WILLIAM (US)
AGUIRRE ANDREW (US)
WOLPIN BRIAN (US)
GALVEZ-REYES JENNYFER (US)
WINTER PETER (US)
NAVIA ANDREW (US)
RAGHAVAN SRIVATSAN (US)
HAHN WILLIAM (US)
AGUIRRE ANDREW (US)
WOLPIN BRIAN (US)
GALVEZ-REYES JENNYFER (US)
Application Number:
PCT/US2020/057342
Publication Date:
June 03, 2021
Filing Date:
October 26, 2020
Export Citation:
Assignee:
DANA FARBER CANCER INST INC (US)
MASSACHUSETTS INST TECHNOLOGY (US)
MASSACHUSETTS INST TECHNOLOGY (US)
International Classes:
A61K35/17; C12N5/071; C12Q1/6869
Foreign References:
| US20190204299A1 | 2019-07-04 | |||
| EP3460042A1 | 2019-03-27 |
Attorney, Agent or Firm:
SMITH, DeAnn, F. et al. (US)
Download PDF:
Previous Patent: CHARITY DONATION AND PAYMENT SYSTEMS
Next Patent: SYSTEMS AND METHODS FOR ANALYZING CASING BONDING IN A WELL USING RADIAL SENSING
Next Patent: SYSTEMS AND METHODS FOR ANALYZING CASING BONDING IN A WELL USING RADIAL SENSING