Title:
PCR DETECTION KIT FOR RAPID DETECTION OF SALMONELLA AND IDENTIFICATION OF SALMONELLA PULLORUM AND SALMONELLA GALLINARUM AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/012037
Kind Code:
A1
Abstract:
The present invention provides a PCR detection kit for detection and identification of Salmonella pullorum/Salmonella gallinarum and a use thereof. By means of designing a primer for a cigR gene, the present invention utilizes PCR detection technology to conduct a cigR genetic test. According to amplification and detection conditions, analysis is conducted to determine whether the test subject is a Salmonella or if the test subject is Salmonella pullorum/Salmonella gallinarum. In specific implementation, the detection kit comprises at least the forward primer shown in SEQ ID NO: 1 and the reverse primer shown in SEQ ID NO: 2.
Inventors:
JIAO XIN'AN (CN)
PAN ZHIMING (CN)
ZHOU YINGYING (CN)
KANG XILONG (CN)
MENG CHUANG (CN)
GU DAN (CN)
YIN JUNLEI (CN)
LI QIUCHUN (CN)
PAN ZHIMING (CN)
ZHOU YINGYING (CN)
KANG XILONG (CN)
MENG CHUANG (CN)
GU DAN (CN)
YIN JUNLEI (CN)
LI QIUCHUN (CN)
Application Number:
PCT/CN2021/075498
Publication Date:
January 20, 2022
Filing Date:
February 05, 2021
Export Citation:
Assignee:
UNIV YANGZHOU (CN)
International Classes:
C12Q1/689; C12Q1/686; C12R1/42
Foreign References:
| CN111733266A | 2020-10-02 |
Other References:
XIA JIE: "The Characteristics of SCV Formation in Salmonella Pullorum-Infected Cells", MASTER THESIS, TIANJIN POLYTECHNIC UNIVERSITY, CN, no. 1, 15 January 2018 (2018-01-15), CN , XP055887518, ISSN: 1674-0246
YIN JUNLEI, XIA JIE, TAO MINGXIN, XU LIJUAN, LI QIUCHUN, GENG SHIZHONG, JIAO XINAN: "Construction and characterization of a cigR deletion mutant of Salmonella enterica serovar Pullorum", AVIAN PATHOLOGY, HUNTINGDON, CAMBS, GB, vol. 45, no. 5, 2 September 2016 (2016-09-02), GB , pages 569 - 575, XP055887520, ISSN: 0307-9457, DOI: 10.1080/03079457.2016.1187708
ZHOU YING-YING, KANG XI-LONG, MENG CHUANG, XIONG DAN, XU YING, GENG SHI-ZHONG, PAN ZHI-MING, JIAO XIN-AN: "Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification", POULTRY SCIENCE, OXFORD UNIVERSITY PRESS, OXFORD, vol. 99, no. 11, 1 November 2020 (2020-11-01), Oxford , pages 5991 - 5998, XP055887521, ISSN: 0032-5791, DOI: 10.1016/j.psj.2020.07.026
YIN JUNLEI, XIA JIE, TAO MINGXIN, XU LIJUAN, LI QIUCHUN, GENG SHIZHONG, JIAO XINAN: "Construction and characterization of a cigR deletion mutant of Salmonella enterica serovar Pullorum", AVIAN PATHOLOGY, HUNTINGDON, CAMBS, GB, vol. 45, no. 5, 2 September 2016 (2016-09-02), GB , pages 569 - 575, XP055887520, ISSN: 0307-9457, DOI: 10.1080/03079457.2016.1187708
ZHOU YING-YING, KANG XI-LONG, MENG CHUANG, XIONG DAN, XU YING, GENG SHI-ZHONG, PAN ZHI-MING, JIAO XIN-AN: "Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification", POULTRY SCIENCE, OXFORD UNIVERSITY PRESS, OXFORD, vol. 99, no. 11, 1 November 2020 (2020-11-01), Oxford , pages 5991 - 5998, XP055887521, ISSN: 0032-5791, DOI: 10.1016/j.psj.2020.07.026
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP) (CN)
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