Title:
PCR PRIMER FOR SEX IDENTIFICATION OF YOUNG PIGEONS, USE THEREOF, AND METHOD FOR SEX IDENTIFICATION OF YOUNG PIGEONS
Document Type and Number:
WIPO Patent Application WO/2024/037252
Kind Code:
A1
Abstract:
Provided are a PCR primer for sex identification of young pigeons, use thereof, and a method for sex identification of young pigeons. The primer is designed for a specific EE0.6 sequence fragment on the W chromosome of female pigeons. During detection, Biotin and FAM are used for modifying the primer. Firstly, an efficient nucleic acid lysis solution is used for rapidly extracting DNA in feather pulp, thereby simplifying the DNA-obtaining process; then specific PCR amplification is carried out by using the modified primer, and an amplified PCR product is used for rapidly identifying female individuals by means of the presence or absence of a band on a colloidal gold test strip. The method has the advantages of high specificity, high accuracy rate, simple and convenient operation, rapid and accurate identification result, small damage to animals, and the like, thereby reducing the time and economic cost of pigeon sex identification, and having wide market application prospects. The detection result of the method is consistent with the result of the colloidal gold test strip, and the accuracy rate is 100%.
Inventors:
YAN LEYAN (CN)
CHEN ZHE (CN)
SUN XUEFENG (CN)
ZHOU HUI (CN)
GUO BINBIN (CN)
DAI ZICHUN (CN)
CHEN ZHE (CN)
SUN XUEFENG (CN)
ZHOU HUI (CN)
GUO BINBIN (CN)
DAI ZICHUN (CN)
Application Number:
PCT/CN2023/106570
Publication Date:
February 22, 2024
Filing Date:
July 10, 2023
Export Citation:
Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
C12Q1/6879; C12N15/11; C12Q1/686; C12Q1/6888
Foreign References:
| CN115287342A | 2022-11-04 | |||
| CN111979308A | 2020-11-24 | |||
| CN111254189A | 2020-06-09 | |||
| CN103255217A | 2013-08-21 | |||
| CN114517236A | 2022-05-20 | |||
| US20110244463A1 | 2011-10-06 |
Other References:
DATABASE Nucleotide 6 February 1999 (1999-02-06), ANONYMOUS : "Columba livia non-repetitive DNA sequence region", XP093140105, retrieved from NCBI Database accession no. D85616.1
AKIRA OGAWA ; IRINA SOLOVEI ; NANCY HUTCHISON ; YASUSHI SAITOH ; JOH-E IKEDA ; HERBERT MACGREGOR ; SHIGEKI MIZUNO: "Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds", CHROMOSOME RESEARCH, KLUWER ACADEMIC PUBLISHERS, DO, vol. 5, no. 2, 1 February 1997 (1997-02-01), Do , pages 93 - 101, XP019234972, ISSN: 1573-6849, DOI: 10.1023/A:1018461906913
WANG ZHIYING, YU WENJIE, XIE RUIBIN, QIAO LU, CHEN AILIANG: "Rapid detection of African swine fever virus based on lateral flow nucleic acid assay", TRANSACTIONS OF THE CHINESE SOCIETY OF AGRICULTURAL ENGINEERING, CHINESE SOCIETY OF AGRICULTURAL ENGINEERING, ZHONGGUO NONGYE GONGCHENG XUEHUI, CN, vol. 36, no. 4, 15 February 2020 (2020-02-15), CN , pages 294 - 299, XP093140113, ISSN: 1002-6819, DOI: 10.11975/j.issn.1002-6819.2020.04.035
AKIRA OGAWA ; IRINA SOLOVEI ; NANCY HUTCHISON ; YASUSHI SAITOH ; JOH-E IKEDA ; HERBERT MACGREGOR ; SHIGEKI MIZUNO: "Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds", CHROMOSOME RESEARCH, KLUWER ACADEMIC PUBLISHERS, DO, vol. 5, no. 2, 1 February 1997 (1997-02-01), Do , pages 93 - 101, XP019234972, ISSN: 1573-6849, DOI: 10.1023/A:1018461906913
WANG ZHIYING, YU WENJIE, XIE RUIBIN, QIAO LU, CHEN AILIANG: "Rapid detection of African swine fever virus based on lateral flow nucleic acid assay", TRANSACTIONS OF THE CHINESE SOCIETY OF AGRICULTURAL ENGINEERING, CHINESE SOCIETY OF AGRICULTURAL ENGINEERING, ZHONGGUO NONGYE GONGCHENG XUEHUI, CN, vol. 36, no. 4, 15 February 2020 (2020-02-15), CN , pages 294 - 299, XP093140113, ISSN: 1002-6819, DOI: 10.11975/j.issn.1002-6819.2020.04.035
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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