Title:
PEPTIDE LIGATION USING ENZYME
Document Type and Number:
WIPO Patent Application WO/2023/048262
Kind Code:
A1
Abstract:
Provided are: a method for producing a ligated peptide, characterized by forming an amide bond between the C-terminal of a donor peptide substrate (donor substrate) and the N-terminal of an acceptor peptide substrate (acceptor substrate) using a penicillin-binding-protein-type thioesterase (PBP-type TE) as a catalyst; and a method for producing a modified peptide, characterized by forming an amide bond between the C-terminal of a donor substrate containing a modification group and the N-terminal of an acceptor substrate using a PBP-type TE as a catalyst.
Inventors:
WAKIMOTO TOSHIYUKI (JP)
MATSUDA KENICHI (JP)
KOBAYASHI MASAKAZU (JP)
MATSUDA KENICHI (JP)
KOBAYASHI MASAKAZU (JP)
Application Number:
PCT/JP2022/035498
Publication Date:
March 30, 2023
Filing Date:
September 22, 2022
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C07K1/02; C12N9/16; C12N9/52
Domestic Patent References:
| WO2019216248A1 | 2019-11-14 |
Other References:
MATSUDA KENICHI, FUJITA KEI, WAKIMOTO TOSHIYUKI: "PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 48, no. 3-4, 4 June 2021 (2021-06-04), GB , pages 23, XP093055066, ISSN: 1367-5435, DOI: 10.1093/jimb/kuab023
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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