Title:
PHASE MODULATION FLUORESCENCE DIFFERENTIAL MICROSCOPIC IMAGING METHOD AND APPARATUS BASED ON ELECTRO-OPTICAL MODULATION TECHNOLOGY
Document Type and Number:
WIPO Patent Application WO/2022/089578
Kind Code:
A1
Abstract:
Disclosed is a phase modulation fluorescence differential microscopic imaging apparatus based on an electro-optical modulation technology. The phase modulation fluorescence differential microscopic imaging apparatus comprises an illumination system for generating an excitation light beam, a detection system for collecting a fluorescence signal emitted by a sample, and a computer for control and signal processing. The illumination system comprises, arranged in sequence, a laser device, used for emitting a laser beam; an electro-optical modulation module, used for controlling rapid conversion of the laser beam between right-handed circularly polarized light and left-handed circularly polarized light; and a phase modulation device, used for performing phase modulation on the circularly polarized light to form a solid light spot and a hollow light spot which illuminate the sample and excite fluorescence. Also disclosed is a phase modulation fluorescence differential microscopic imaging method based on the electro-optical modulation technology, rapid conversion of solid and hollow light spots is achieved, and an imaging speed of a fluorescence differential microscopic imaging system is increased to a great extent.
Inventors:
KUANG CUIFANG (CN)
LIU XU (CN)
DONG WANJIE (CN)
LIU SHAOCONG (CN)
LIU XU (CN)
DONG WANJIE (CN)
LIU SHAOCONG (CN)
Application Number:
PCT/CN2021/127388
Publication Date:
May 05, 2022
Filing Date:
October 29, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
G02B21/06; G01N21/64; G02B21/00
Foreign References:
| CN112711130A | 2021-04-27 | |||
| US20200310095A1 | 2020-10-01 | |||
| CN109283674A | 2019-01-29 | |||
| CN111024659A | 2020-04-17 | |||
| CN109387496A | 2019-02-26 |
Other References:
YOU SHANGTING, KUANG CUIFANG, RONG ZIHAO, LIU XU: "Eliminating deformations in fluorescence emission difference microscopy", OPTICS EXPRESS, SPRINGERSPRINGERSPIE, vol. 22, no. 21, 20 October 2014 (2014-10-20), pages 26375 - 64, XP055927209, DOI: 10.1364/OE.22.026375
Attorney, Agent or Firm:
HANGZHOU TIANQIN INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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