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Patent Searching and Data


Title:
PHOSPHOLIPASE C AND ENCODING GENE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/114645
Kind Code:
A1
Abstract:
According to the present invention, on the basis of all existing mutations, the tenth glycine of a BC-PC-PLC is mutated into aspartic acid, a specific enzyme activity thereof is 83% higher than that of a sequence before the mutation, and protein expression and degumming activity of unit enzyme activity do not change, so as to further reduce manufacturing costs.

Inventors:
WU WEI (CN)
DAI XIAOJUN (CN)
CAO HAISHENG (CN)
NIU QIWEN (CN)
Application Number:
PCT/CN2018/120026
Publication Date:
June 20, 2019
Filing Date:
December 10, 2018
Export Citation:
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Assignee:
WILMAR SHANGHAI BIOTECHNOLOGY RES & DEV CT CO LTD (CN)
International Classes:
C12N9/20; C12N9/16; C12N15/55; C11B3/00; C12N15/81
Foreign References:
CN106884009A2017-06-23
CN104630174A2015-05-20
CN101558154A2009-10-14
CN104630174A2015-05-20
CN201610030U2010-10-20
Other References:
JOHANSEN, T.HOLM,T.GUDDAL, P.H.SLETTEN, K.HAUGLI, F.B.LITTLE, C.: "Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus", GENE, vol. 65, no. 2, 1988, pages 293 - 304, XP025705183, DOI: 10.1016/0378-1119(88)90466-0
HOUGH., E.HANSEN, L.K.BIRKNES, B.JYNGE, K.HANSEN, S.HORDVIK, A.LITTLE, C.DODSON, E.DEREWENDA, Z.: "High-resolution (1.5A) crystal structure of phospholipase C from Bacillus cereus", NATURE, vol. 338, 1989, pages 357 - 60
"Computational Molecular Biology", 1988, OXFORD UNIVERSITY PRESS
"Biocomputing: Informatics and Genome Projects", 1993, ACADEMIC PRESS
HIGGINS ET AL., NUCLEIC ACIDS RES., vol. 22, 1994, pages 4673 - 4680
"Sequence Analysis in Molecular Biology", 1987, ACADEMIC PRESS
"Sequence Analysis Primer", 1991, STOCKTON PRESS
EMBL-EBI; RICE, TRENDS IN GENETICS, vol. 16, no. 6, 2000, pages 276 - 277
HIGGINSSHARP, CABIOS, vol. 5, 1989, pages 151 - 153
CHENNA ET AL., NUCLEIC ACIDS RES, vol. 31, no. 13, 2003, pages 3497 - 500
THOMAS D.: "Brock in Biotechnology: A Textbook of Industrial Microbiology", 1989, SINAUER ASSOCIATES, INC.
DESHPANDE, MUKUND V., APPL. BIOCHEM. BIOTECHNOL., vol. 36, 1992, pages 227 - 234
See also references of EP 3725878A4
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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