Title:
PHOSPHOLIPASE D MUTANT, USE THEREOF AND METHOD FOR PREPARING PHOSPHATIDYLSERINE BY MEANS OF SAME
Document Type and Number:
WIPO Patent Application WO/2020/133314
Kind Code:
A1
Abstract:
Disclosed are a phospholipase D mutant, a use thereof and a method for preparing phosphatidylserine by means of same, which relate to the technical field of bio-enzyme catalysis. Provided is a phospholipase D mutant with an enzyme activity inclined more towards a phosphatidyl transfer activity. Compared with the amino acid sequence of wild-type phospholipase D, the phospholipase D mutant has a mutation at at least one position selected from positions 93, 105, 215, 216, 228 and 486.
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Inventors:
FU RONGZHAO (CN)
LI ZHENWEI (CN)
LIU WENSHAN (CN)
TANG XIAOXIN (CN)
ZHU ZHIQIANG (CN)
LI ZHENWEI (CN)
LIU WENSHAN (CN)
TANG XIAOXIN (CN)
ZHU ZHIQIANG (CN)
Application Number:
PCT/CN2018/125222
Publication Date:
July 02, 2020
Filing Date:
December 29, 2018
Export Citation:
Assignee:
BONTAC BIO ENG SHENZHEN CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
JIANGXI BONTAC GREEN BIOCATALYSIS ECOINDUSTRIAL PARK CO LTD (CN)
International Classes:
C12N15/00; C12N9/16; C12N15/55; C12R1/465
Foreign References:
| CN106085984A | 2016-11-09 | |||
| CN106957850A | 2017-07-18 | |||
| CN102286440A | 2011-12-21 | |||
| CN101379187A | 2009-03-04 | |||
| CN108220267A | 2018-06-29 | |||
| CN107090416A | 2017-08-25 |
Other References:
DATABASE Nucleotide 26 July 2016 (2016-07-26), OGINO, C. ET AL.: "Streptoverticillium cinnamoneum gene for Phospholipase D, complete cds", XP055722824, retrieved from ncbi Database accession no. AB007132.1
LU , XINRUI ET AL.: "PSS (Designatted Mutation of Phosphatidylserrine Synthetase (PSS) and Its Enzymatic Activity", JOURNAL OF MICROBIOLOGY, vol. 34, no. 6, 15 December 2014 (2014-12-15), pages 94 - 97
LU , XINRUI ET AL.: "PSS (Designatted Mutation of Phosphatidylserrine Synthetase (PSS) and Its Enzymatic Activity", JOURNAL OF MICROBIOLOGY, vol. 34, no. 6, 15 December 2014 (2014-12-15), pages 94 - 97
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