Title:
PHOSPHOLIPID SCRAMBLASES AND METHODS OF USE THEREOF
Document Type and Number:
WIPO Patent Application WO2001074295
Kind Code:
A3
Abstract:
The present invention is based on a family of membrane proteins, Phospholipid Scramblases (PLSCR), that mediate accelerated trans-bilayer movement of plasma membrane phospholipids in response to elevated cytoplasmic calcium. At least one Phospholipid Scramblase gene is highly inducible by interferon. Interferon-induced expression of Phospholipid Scramblase 1 (and/or related genes) alters the physical and functional properties of the cell surface so as to (1) inhibit tumor cell proliferation and survival; (2) inhibit maturation and release of membrane-enveloped viruses; and/or (3) promote clearance of virus-infected cells and cancer cells through the reticuloendothelial system. The present invention provides Phospholipid Scramblase polypeptides, polynucleotide sequences that encode Phospholipid Scramblase polypeptides, and antibodies that are immunoreactive with the polypeptides. The finding that human Phospholipid Scramblase 1 polypeptides are induced by interferons, indicates a role for the Scramblase polypeptides in treating and preventing cancer and viral infection.
Inventors:
SIMS PETER J (US)
SILVERMAN ROBERT H (US)
WIEDMER THERESE (US)
SILVERMAN ROBERT H (US)
WIEDMER THERESE (US)
Application Number:
PCT/US2001/010388
Publication Date:
February 21, 2002
Filing Date:
March 30, 2001
Export Citation:
Assignee:
SCRIPPS RESEARCH INST (US)
CLEVELAND CLINIC FOUNDATION (US)
SIMS PETER J (US)
SILVERMAN ROBERT H (US)
WIEDMER THERESE (US)
CLEVELAND CLINIC FOUNDATION (US)
SIMS PETER J (US)
SILVERMAN ROBERT H (US)
WIEDMER THERESE (US)
International Classes:
A61P31/12; A61P43/00; C07K14/47; (IPC1-7): C12N9/00; C12N1/20; C12N15/00; C07H21/04; A61K38/00
Other References:
WIEDMER T. ET AL.: "Identification of three new members of the phospholipid sramblase gene family", BIOCHIM. BIOPHYS. ACTA, vol. 1467, July 2000 (2000-07-01), pages 244 - 253, XP002949661
BASSE F. ET AL.: "Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid", J. BIOL. CHEM., vol. 271, no. 29, 19 July 1996 (1996-07-19), pages 17205 - 17210, XP002949662
BASSE F. ET AL.: "Isolation of an erythrocyte membrane protein that mediates Ca2+-dependent transbilayer movement of phospholipid", J. BIOL. CHEM., vol. 271, no. 29, 19 July 1996 (1996-07-19), pages 17205 - 17210, XP002949662
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