Title:
PLANT WITH REDUCED PROTEIN CONTENT IN SEED, METHOD OF CONSTRUCTING THE SAME AND METHOD OF USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2004/056993
Kind Code:
A1
Abstract:
It is intended to provide a method of reducing the total amount of stored protein, to develop a technique required therefor, and to provide a plant and its seeds developed by the above method and a method of using such a plant and seeds. More specifically, a nucleic acid molecule containing a consecutive nucleic acid sequence of at least 15 in length which is complementary with a nucleic acid sequence encoding prolamine polypeptide or a nucleic acid sequence having a homology of at least about 70% to the complementary nucleic acid sequence having at least 15 nucleotide length. A method of reducing the expression dose of a protein in a seed of a plant which comprises: A) the step of providing the above-described nucleic acid molecule; B) the step o transferring the nucleic acid molecule into the cells of the plant; C) the step of re-differentiating the cells to construct a transgenic plant; and D) the step of obtaining seeds from the transgenic plant.
Inventors:
KURODA MASAHARU (JP)
Application Number:
PCT/JP2003/015753
Publication Date:
July 08, 2004
Filing Date:
December 09, 2003
Export Citation:
Assignee:
INC ADMIN AGENCY NARO (JP)
KURODA MASAHARU (JP)
KURODA MASAHARU (JP)
International Classes:
A23L7/10; C07K14/415; C12N15/82; (IPC1-7): C12N15/09; A01H5/00
Domestic Patent References:
| WO2002016604A1 | 2002-02-28 | |||
| WO1993018643A1 | 1993-09-30 | |||
| WO2002016604A1 | 2002-02-28 | |||
| WO1993018643A1 | 1993-09-30 |
Foreign References:
| EP1210869A1 | 2002-06-05 |
Other References:
GOOSSENS A, ET AL: "CO-INTRODUCTION OF AN ANTISENSE GENE FOR AN ENDOGENOUS SEED STORAGE PROTEIN CAN INCREASE EXPRESSION FOR A TRANSGENE IN ARABIDOPSIS THALIANA SEEDS", FEBS LETTERS, vol. 456, 1999, pages 160 - 164, XP004260058
KIM W T, ET AL: "NUCLEOTIDE AND PRIMARY SEQUENCE OF A MAJOR RICE PROLAMINE", FEBS LETTERS, vol. 231, 1988, pages 308 - 310, XP002974672
WESLEY S V, ET AL: "CONSTRUCT DESIGN FOR EFFICIENT, EFFECTIVE AND HIGH-THROUGHPUT GENE SILENCING IN PLANTS", PLANT J., vol. 2, no. 6, 2001, pages 581 - 590, XP002187670
MARUTA Y, ET AL: "TRANSGENIC RICE WITH REDUCED GLUTELIN CONTENT BY TRANSFORMATION WITH GLUTELIN A ANTISENSE GENE", MOLECULAR BREEDING, vol. 8, 2001, pages 273 - 284, XP002974673
IIDA S, ET AL: "MUTANTS LACKING GLUTELIN SUBUNITS IN RICE: MAPPING AND COMBINATION OF MUTATED GLUTELIN GENES", THEOR. APPL. GENET., vol. 94, 1997, pages 177 - 183, XP002974674
HUANG SHIHSHIEH, PLANT BIOLOGY (ROCKVILLE, vol. 2002, pages 106
GOOSSENS, A. ET AL., FEBS LETTERS, vol. 456, 1999, pages 160 - 164
WESLEY, S. V. ET AL., PLANT J., vol. 2, no. 6, 2001, pages 581 - 590
KIM W T, ET AL: "NUCLEOTIDE AND PRIMARY SEQUENCE OF A MAJOR RICE PROLAMINE", FEBS LETTERS, vol. 231, 1988, pages 308 - 310, XP002974672
WESLEY S V, ET AL: "CONSTRUCT DESIGN FOR EFFICIENT, EFFECTIVE AND HIGH-THROUGHPUT GENE SILENCING IN PLANTS", PLANT J., vol. 2, no. 6, 2001, pages 581 - 590, XP002187670
MARUTA Y, ET AL: "TRANSGENIC RICE WITH REDUCED GLUTELIN CONTENT BY TRANSFORMATION WITH GLUTELIN A ANTISENSE GENE", MOLECULAR BREEDING, vol. 8, 2001, pages 273 - 284, XP002974673
IIDA S, ET AL: "MUTANTS LACKING GLUTELIN SUBUNITS IN RICE: MAPPING AND COMBINATION OF MUTATED GLUTELIN GENES", THEOR. APPL. GENET., vol. 94, 1997, pages 177 - 183, XP002974674
HUANG SHIHSHIEH, PLANT BIOLOGY (ROCKVILLE, vol. 2002, pages 106
GOOSSENS, A. ET AL., FEBS LETTERS, vol. 456, 1999, pages 160 - 164
WESLEY, S. V. ET AL., PLANT J., vol. 2, no. 6, 2001, pages 581 - 590
Attorney, Agent or Firm:
Yamamoto, Shusaku (Crystal Tower 2-27, Shiromi 1-chome, Chuo-k, Osaka-shi Osaka, JP)
Download PDF: