PLASMID VECTORS FOR IN VIVO SELECTION-FREE USE WITH THE PROBIOTIC E. COLI NISSLE

Title:

PLASMID VECTORS FOR IN VIVO SELECTION-FREE USE WITH THE PROBIOTIC E. COLI NISSLE

Document Type and Number:

WIPO Patent Application WO/2022/060848

Kind Code:

A8

Abstract:

Disclosed are methods for producing genetically modified bacteria, comprising introducing into said bacteria at least one engineered cryptic plasmid comprising a heterologous nucleic acid, wherein the heterologous nucleic acid comprises a nucleic acid sequence encoding a recombinant protein and a polypeptide secretion system for directing the recombinant protein to the outer membrane for secretion, wherein the bacteria do not comprise any native cryptic plasmids.

Inventors:

JOSHI NEEL (US)
GELFAT ILIA (US)
KAN ANTON (US)

Application Number:

PCT/US2021/050479

Publication Date:

February 16, 2023

Filing Date:

September 15, 2021

Export Citation:

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Assignee:

UNIV NORTHEASTERN (US)
HARVARD COLLEGE (US)

International Classes:

C12N15/74

Attorney, Agent or Firm:

GORDON, Dana, M. et al. (US)

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