Title:
PLASTID TRANSFORMATION
Document Type and Number:
WIPO Patent Application WO2004035734
Kind Code:
A3
Abstract:
The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and two phases of selection with first selection phase using a nonlethal compound and a second selection phase using a lethal compound. Alternatively, the dual selection method is conducted simultaneously using a lower concentration of the lethal compound.
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Inventors:
BOUDREAU ERIC (US)
GU WEINING (US)
DE FRAMOND ANIC (US)
HEIFETZ PETER (US)
GU WEINING (US)
DE FRAMOND ANIC (US)
HEIFETZ PETER (US)
Application Number:
PCT/US2003/031941
Publication Date:
February 17, 2005
Filing Date:
October 07, 2003
Export Citation:
Assignee:
SYNGENTA PARTICIPATIONS AG (CH)
BOUDREAU ERIC (US)
GU WEINING (US)
DE FRAMOND ANIC (US)
HEIFETZ PETER (US)
BOUDREAU ERIC (US)
GU WEINING (US)
DE FRAMOND ANIC (US)
HEIFETZ PETER (US)
International Classes:
C12N15/82; (IPC1-7): C12N15/82
Domestic Patent References:
| WO2001068826A2 | 2001-09-20 |
Foreign References:
| US20020042934A1 | 2002-04-11 |
Other References:
KINDLE ET AL.: "Engineering the chloroplast genome: techniques and capabilities for chloroplast trasnformation in Chlamydomonas reinhardtii", PROC. NATL. ACAD. SCI. USA, vol. 88, March 1991 (1991-03-01), pages 1721 - 1725, XP002982905
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