PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS

Title:

PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS

Document Type and Number:

WIPO Patent Application WO/2019/009364

Kind Code:

A1

Abstract:

Provided are a method and an apparatus for stably producing sound and functional platelets and a method for determining operating conditions in the platelet production apparatus. The present invention relates to a production method including a step for culturing megakaryocyte cells in a platelet production medium C, wherein the culturing step includes a step for stirring the medium C in containers 1, 4 by using stirring mechanisms 2, 5. In one production method, the stirring step involves reciprocating agitating blades 21, 24 of the stirring mechanism 2 so as to generate a predetermined turbulent flow energy, a shear stress, or a Kolmogorov scale in the medium C. In another production method, the stirring step involves rotating a stirring blade 51 of the stirring mechanism 5 in a state in which a plurality of stationary members 53 is arranged therearound in a bottom region 4e of the container 4 so that a turbulent flow is generated in the medium C. The present invention also relates to a production apparatus for carrying out the production method. The present invention also relates to a method for determining operating conditions in the platelet production apparatus.

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Inventors:

ETO KOJI (JP)
NAKAMURA SOU (JP)
ITO YUKITAKA (JP)
SHIGEMORI TOMOHIRO (JP)
KATO YOSHIKAZU (JP)

Application Number:

PCT/JP2018/025537

Publication Date:

January 10, 2019

Filing Date:

July 05, 2018

Export Citation:

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Assignee:

UNIV KYOTO (JP)
MEGAKARYON CORP (JP)
SATAKE CHEM EQUIP MFG (JP)

International Classes:

C12N5/078; B01F7/16; B01F7/18; B01F11/00; C12M1/02; C12M3/00; C12N5/10

Domestic Patent References:

WO2017077964A1	2017-05-11
WO2013187359A1	2013-12-19
WO2012157586A1	2012-11-22
WO2017077964A1	2017-05-11
WO2011034073A1	2011-03-24
WO2014123242A1	2014-08-14

Foreign References:

JP2006043668A	2006-02-16
JP2006296423A	2006-11-02
JPS61149080A	1986-07-07
JP2016021908A	2016-02-08
US20120238023A1	2012-09-20
US20140127815A1	2014-05-08
US20160002599A1	2016-01-07

Other References:

SCIENCE, vol. 317, no. 5845, pages 1767 - 1770
BLOOD, vol. 127, no. 7, 2016, pages 921 - 926
OGURA ET AL., REGENERATIVE MEDICINE, vol. 6, no. 4, pages 26 - 32
JSEUS ET AL., NATURE REVIEWS MOLECULAR CELL BIOLOGY, vol. 7, 2006, pages 667 - 677
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NAKAMURA S ET AL., CELL STEM CELL, vol. 14, 2014, pages 535 - 548
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NAKAMURA ET AL., CELL STEM CELL, vol. 14, no. 4, 3 April 2014 (2014-04-03), pages 535 - 48
NAKAMURA ET AL., CELL CULTURE
TAKAYAMA ET AL., BLOOD, vol. 111, no. 11, 1 June 2008 (2008-06-01), pages 5298 - 306
HIRATA ET AL., STEM CELL TRANSLATIONAL MEDICINE
See also references of EP 3650535A4

Attorney, Agent or Firm:

OKUYAMA, Shoichi et al. (JP)

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