Title:
PNA PROBE AND PRIMER FOR DETECTION OF SARS-COV-2 CAUSING COVID-19 BY RT-LAMP, AND METHOD FOR DETERMINING INFECTION BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/201601
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting SARS-CoV-2 causing COVID-19 by RT-LAMP and, more specifically, to a PNA probe and primer for the detection of SARS-CoV-2 by RT-LAMP and a method for determining infection by using same. According to the present invention, the method is more convenient than conventional PCR and RT-PCR methods used in COVID-19 diagnosis, and can rapidly amplify and determine an ORF1ab gene and an N gene of SARS-CoV-2, and thus, the method can rapidly diagnose infections with SARS-CoV-2.
Inventors:
LEE SI SEOK (KR)
KIM HAN GYEUL (KR)
LEE HAN WOO (KR)
PARK HEE KYUNG (KR)
KIM HAN GYEUL (KR)
LEE HAN WOO (KR)
PARK HEE KYUNG (KR)
Application Number:
PCT/KR2021/004009
Publication Date:
October 07, 2021
Filing Date:
March 31, 2021
Export Citation:
Assignee:
SEASUN BIOMATERIALS (KR)
International Classes:
C12Q1/70; C12Q1/6818
Foreign References:
| US20080044814A1 | 2008-02-21 |
Other References:
WEIHUA YANG, XIAOFEI DANG, QINGXI WANG, MINGJIE XU, QIANQIAN ZHAO, YUNYING ZHOU, HUAILONG ZHAO, LI WANG, YIHUI XU, JUN WANG, SHUYI: "Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method", MEDRXIV, 3 March 2020 (2020-03-03), pages 1 - 25, XP055733463, DOI: 10.1101/2020.03.02.20030130
RAO ARUNDHATI, WOLK DONNA M, GOLDSTEIN D Y, WOLF LESLIE A, SCOTT BAYLOR, HEALTH WHITE: "Development and Evaluation of Two SARS-CoV-2 RT-PCR Laboratory Developed Tests on the ARIES ® Automated, Sample-to-Answer, Real-Time PCR System", 1 March 2020 (2020-03-01), pages 1 - 9, XP055855620, Retrieved from the Internet [retrieved on 20211027]
DATABASE NUCLEOTIDE 11 February 2020 (2020-02-11), ANONYMOUS: "Severe acute respiratory syndrome coronavirus 2 isolate 2019-nCoV WHU01, complete genome", XP055855625, retrieved from GENBANK Database accession no. MN988668
KIM JIN HWA; KANG MINHEE; PARK EUNKYOUNG; CHUNG DOO RYEON; KIM JIYEON; HWANG EUNG SOO: "A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV", BIOCHIP JOURNAL, vol. 13, no. 4, 1 January 1900 (1900-01-01), Seoul , South Korea, pages 341 - 351, XP037382102, ISSN: 1976-0280, DOI: 10.1007/s13206-019-3404-3
YINHUA ZHANG, NELSON ODIWUOR, JIN XIONG, LUO SUN, RAPHAEL OHURU NYARUABA, HONGPING WEI, NATHAN A TANNER: "Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP", MEDRXIV, 29 February 2020 (2020-02-29), pages 1 - 14, XP055730127, DOI: 10.1101/2020.02.26.20028373
RAO ARUNDHATI, WOLK DONNA M, GOLDSTEIN D Y, WOLF LESLIE A, SCOTT BAYLOR, HEALTH WHITE: "Development and Evaluation of Two SARS-CoV-2 RT-PCR Laboratory Developed Tests on the ARIES ® Automated, Sample-to-Answer, Real-Time PCR System", 1 March 2020 (2020-03-01), pages 1 - 9, XP055855620, Retrieved from the Internet
DATABASE NUCLEOTIDE 11 February 2020 (2020-02-11), ANONYMOUS: "Severe acute respiratory syndrome coronavirus 2 isolate 2019-nCoV WHU01, complete genome", XP055855625, retrieved from GENBANK Database accession no. MN988668
KIM JIN HWA; KANG MINHEE; PARK EUNKYOUNG; CHUNG DOO RYEON; KIM JIYEON; HWANG EUNG SOO: "A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV", BIOCHIP JOURNAL, vol. 13, no. 4, 1 January 1900 (1900-01-01), Seoul , South Korea, pages 341 - 351, XP037382102, ISSN: 1976-0280, DOI: 10.1007/s13206-019-3404-3
YINHUA ZHANG, NELSON ODIWUOR, JIN XIONG, LUO SUN, RAPHAEL OHURU NYARUABA, HONGPING WEI, NATHAN A TANNER: "Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP", MEDRXIV, 29 February 2020 (2020-02-29), pages 1 - 14, XP055730127, DOI: 10.1101/2020.02.26.20028373
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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