FIELD OF THE INVENTION

The invention relates to novel polycyclic alkoloids which can bind or antagonize the ΝMDA (Ν-methyl- (D) -aspartic acid) receptor complex or otherwise protect neurons against excitatory amino acid receptor-induced degeneration. In another aspect, the invention relates to a method of inhibiting ΝMDA receptor activation in- a mammal using the novel polycyclic alkaloids of the invention.

5 BACKGROUND OF THE INVENTION

Excitatory amino acids such as L-glutamate (Glu) and L- aspartate (Asp) , are major neurotransmitters in the mammalian central nervous system. Multiple acidic amino 0 acid receptor subtypes exist for these acid amino acid neurotransmitters. For example, these include ion channel-linked receptors mediating neuronal depolarization, named after the protypical agonists N- methyl-D-aspartate (NMDA) , alpha-amino-5-methyl-4- 5 isoxazoleproprionic acid (AMPA) , kainate and a putative presynaptic stimulator, L-2-amino-4-phosphonobutyrate (L- AP4) . A fifth excitatory amino acid receptor is the metabotropic receptor, linked to phosphoinositide metabolism (Farooqui and Horrocks, Brain Res. Rev. 16,

30 171, 1991) .

NMDA receptors play a specialized role due to the unique properties of their linked ion channels and participate in various plastic neuronal events including initiation of long-term potentiation, which is a proposed substrate of

learning and memory and the establishing of synaptic contacts during neuronal development. NMDA receptors are also involved in other processes such as the transmission of sensory information (MacDermott and Dale, Trends Neurosci. 1C>, 280, 1987) .

Apart from their important physiological roles, excitatory acidic amino acids such as NMDA are also involved in pathophysiological events in the central nervous system.

la

Abnormally low levels of glutamic acid (Glu) can compromise normal levels of excitation and cause, for example, learning and memory deficits. Excessive levels of Glu can produce toxic effects. The term "excitotoxicity" was coined by Olney (in Hyhan W.L. [ed] : "Heritage Disorders of Amino Acids Metabolism" New York: Macmillan pp. 501-512, 1989) to describe the process by which excitatory amino acids can cause neuronal cell death.

Evidence indicates that NMDA receptors exist in the peripheral tissues and that activation of these receptors may be involved in a mechanism of lung and other organ injury (Said, S.I. et al. , Letters to Neuroscience, 65, 943-946, 1995) . This cytotoxic process is mainly mediated by an over-stimulation of NMDA receptors and may occur in cases of cerebral stroke, cerebral ischaemia, epilepsy, Alzheimer's disease, AIDS-related dementias, traumatic brain injury and other neurodegenerative disorders (Olney, Ann. Rev. Pharmacol. Toxicol. 3_2 _. ^: 47-71, 1990; Foster et al, in "Current and future Trends in Anticonvulsant, Anxiety and Stroke Therapy" Wiley-Liss, Inc. pp. 301-329, 1990; Rogawski and Porter, Pharmacol. Rev., 42: 223-286, 1990) .

The NMDA receptor comprises several binding domains that interact with each other for proper functioning and modulation of nerve cell activity. It is theorized that the NMDA receptor forms a complex acting as a receptor- linked ion channel. Essentially, the function of the receptor is to bind NMDA or the natural amino acids, Glu or Asp, and open an associated ion channel that allows the entry of sodium (Na ⁺ ) and calcium (Ca ²⁺ ) into the stimulated neuron as well as the exit of potassium (K ⁺ ) .

Whereas the ion channels of other excitatory amino acid receptors (AMPA, kainate and L-AP4) are only permeable to Na ⁺ and K ⁺ , the NMDA receptor channel is also permeable to Ca ²⁺ . This feature may be of importance for the proposed role of this receptor in both short and long-term plasticity such as learning, memory and neuropathology.

Intracellular Ca ⁺ is responsible for the regulation of a large variety of cellular activities (Faroogui and Horrocks, Brain Res. Rev. _16, 171; 1991) . An overstimulation of brain NMDA receptors, observed in cases of anoxia, ischaemia and hypoglycemia, results in a build¬ up of the concentration of Ca++ in stimulated neurons and a cascade of intracellular events (activation of phospholipases [PLA2, PLC], lipases, proteases and endonucieases) that lead to neuronal cell death (Farooqui and Horrocks, Brain Res. Rev. JL6, 171; 1991) .

There is therefore a need for compounds which can bind or antagonize the NMDA receptor complex or otherwise protect neurons against excitatory amino acid receptor-induced degeneration.

SUMMARY OF THE INVENTION

The present invention relates to novel polycyclic alkaloids that act as antagonists at the ionotropic NMDA (N-methyl- (D) -aspartic acid) receptor having the general structure represented by formula I:

formula I

wherein Ri is H, Cι-6 alkyl, or C ₆ -ι ₂ aryl optionally substituted with polar groups;

R ₂ and R ₃ are independently H, OH, Cι_ ₆ alkyl, -C(NH)-NH ₂ , a positively charged group, or C ₇ _ _i3 aralkyl optionally substituted with NH ₂ , OH, Cι_ ₆ alkyl, or halogen; or R ₂ and R ₃ together form a 5 to 6 member ring optionally incorporating a heteroatom;

R ₄ is H, Cι-6 alkyl, OR ₆ , SR ₆ or N(Rε)2/ wherein each R ₆ is independently H or Cι_ ₃ alkyl;

X is 0, S, SO, S0 ₂ , N-R ₅ , or C-(R ₅ ) ₂ , wherein each R is independently H, Cι_ ₆ alkyl, or C ₇ _ _i3 aralkyl optionally interrupted with one or more heteroatom; n is an integer from 0 to 2; m is an integer from 0 to 3 ; with the proviso that when X is CH ₂ then R _x is not CH ₃ , R ₂ and R ₃ are not both H, R ₄ is not OH, m is not 3 and n is not 0.

It will be appreciated by those skilled in the art that the compounds of formula (I) , depending of the substituents, may contain one or more chiral centers and thus exist in the form of many different isomers, optical isomers (i.e. enantiomers) and mixtures thereof including racemic mixtures. All such isomers, enantiomers and mixtures thereof including racemic mixtures are included within the scope of the invention.

In another aspect of the present invention there is provided a method of inhibiting NMDA receptor activation in a mammal comprising administering to said mammal an NMDA receptor antagonizing amount of a compound according to claim 1.

In another aspect, there is provided a method of treating or preventing cell damage or cytotoxicity mediated by NMDA receptor activation in a mammal comprising administering to said mammal a pharmaceutically effective amount of a compound according to claim 1.

In a further aspect of the invention, there are provided methods of treating or preventing neuro degenerative disease, stroke, epileptic seizures and convulsions in a mammal comprising administering to said mammal a pharmaceutically effective amount of a compound according to claim 1.

BRIEF DESCRIPTION OF THE FIGURES

Figure 1 depicts a dose dependent neuroprotectant activity effect of dextrorphan following NMDA challenge in mice.

Figure 2 depicts a dose dependent neuroprotectant activity effect of compound #13 following NMDA challenge in mice.

Figure 3 depicts a dose dependent neuroprotectant activity effect of compound #9 following NMDA challenge in mice.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to novel polycyclic alkaloids according to formula I :

formula I

wherein Rι~R ₄ , X, m and n are as previously defined.

The following common abbreviations are used throughout the specification:

'EAA' refers to excitatory amino acid. ^λ NMDA' refers to N-methyl- (D) -aspartic acid. 'AMPA' refers to alpha-amino-5-methyl-4-isoxazole- proprionic acid.

The term CD ₅₀ as shown in Table 1 is defined as the dose of drug which induces a 50% reduction in the number of deaths caused by NMDA administration.

As used in this application, the term 'alkyl' represents a saturated or unsaturated, substituted (by one or more halogen, hydroxyl, amino, or C ₆ - ₂₀ aryl group) or unsubstituted; straight chain, branched chain, or cyclic hydrocarbon moiety wherein said straight chain, branched chain, or cyclic hydrocarbon moiety can be interrupted by one or more heteroatoms (such as oxygen, nitrogen or sulfur) .

The term 'aryl' represents a carbocyclic moiety which is optionally substituted (e.g. one or more Cι_ ₆ alkyl, halogen, hydroxyl, amino) , interrupted by one or more heteroatoms (e.g., N, 0 or S) and containing at least one benzenoid-type ring (e.g. phenyl and napthyl) .

The term 'aralkyl' represents an aryl group attached to the adjacent atom by an alkyl (e.g. benzyl) .

The compounds of the present invention are represented by formula (I) as defined above.

Preferably, Ri is cyclohexyl.

Preferably, Ri is phenyl optionally substituted with polar groups.

Preferred polar groups are COOH, NH ₂ or guanidino. More preferably, Ri is H. Most preferably, Ri is CH ₃ .

Preferably, R ₂ -C(NH)-NH ₂ .

Preferably, R ₂ is a positively charged amino group. More preferably, R ₂ is H.

Preferably R ₃ is Cι_ ₆ alkyl . More preferably, R ₃ is OCH ₃ . Most preferably, R ₃ is H. Most preferably, R ₃ is OH.

Preferably R ₄ is OCι_ ₆ alkyl Preferably R ₄ is halogen. More preferably, R ₄ is OH. Most preferably, R ₄ is OCH ₃ .

Preferably, X is SO.

Preferably, X is S0 ₂ .

Preferably, X is NR ₅ .

More preferably, X is 0. Most preferably, X is S.

Preferably s Cι- ₆ alkyl. More preferably, R ₅ is CH ₃ Most preferably, R ₅ is H.

Preferably n is 0. Preferably, m is 3.

A preferred compound of the invention includes: compound #8a: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- methoxy -5-methyl -10- thia -5, 11- methanobenzocyclodecen -13- β-hydroxylamine;

A preferred compound of the invention includes: compound #8b: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- methoxy -5- methyl -10- thia - 5, 11 methanobenocyclodecen -13- amine;

A preferred compound of the invention includes: compound #9: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy - 5- methyl -10- thia -5, li¬ methanobenzocyclodecen -13- amine (sulphazocine) ;

A preferred compound of the invention includes : compound #9a (-) -trans-5, 6, 7, 8, 9, 11, 12-heptahydro- 10-thia-3-hydroxy-5-methyl-5, li¬ methanobenzocyclodecen-13-amine;

A preferred compound of. the invention includes: compound #9b (+) -trans-5, 6, 7, 8, 9, 11, 12-heptahydro-10- thia-3-hydroxy-5-methyl-5, 11-methanobenzocyclodecen- 13 amine;

A preferred compound of the invention includes: compound #10: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy -5- methyl -10- thia -5, li¬ methanobenzocyclodecene -13- β-hydroxylamine;

A preferred compound of the invention includes: compound #11 trans-5, 6,7, 8, 9,11,12-heptahydro-10- thia-3-hydroxy-5-methyl-5,11-methanobenzocyclodecen- 13-guanidine. A preferred compound ^" of the invention includes: compound #12 trans-5, 6, 7, 8,9, 11, 12-heptahydro-10- sulphono-3-hydroxy-5-methyl-5,11-methanobenzocyclo- decen-13-amine; A preferred compound of the invention includes: compound #13: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy -5- methyl -5, 11- methanobenzocyclodecene - 13- amine.

A more preferred compound of the invention is: compound #11: trans-5,6,7,8,9,11,12-heptahydro-10- thia-3-hydroxy-5-methyl-5, 11-methanobenzocyclodecen- 13-guanidine.

A most preferred compound of the invention is: compound #9: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- ^* ydroxy -5- methyl -10- thia - 5,11 methanobenzocyclodecen -13- amine (sulphazocine)

A most preferred compound of the invention is:

RECTIFIED SHEET (RULE 91) ISA/EP

compound #10: 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy -5- methyl -10- thia -5, 11- methanobenzocyclodecene -13- β-hydroxylamine;

A most preferred compound of the invention is: compound #11 trans-5, 6, 7, 8, 9, 11, 12-heptahydro-10- thia-3-hydroxy-5-methyl-5, 11-methanobenzocyclodecen- 13-guanidine. The compounds of the present invention can be synthesized using conventional preparative steps and recovery methods known to those skilled in the art of organic and bio¬ organic synthesis, while providing a new and unique combinations for the overall synthesis of each compound. Preferred synthetic routes for intermediates involved in the synthesis as well as the resulting NMDA receptor antagonist compounds of the present invention follow. Successful preparation of these compounds is possible by way of several synthetic routes one of which is outlined in Scheme 1.

10

SCHEME 1

XII

The steps illustrated in Scheme 1 can be briefly described as follows:

Step 1: Compound I, an alkyl-1-tetralone, is treated with an appropriate Grignard Reagent such as methyl magnesium

11

bromide in a dry non-polar solvent such as THF, to generate the tertiary alcohol, Compound II.

Step 2 : The alcohol, Compound II is dehydrated under acidic conditions, such as aqueous saturated NH ₄ C1, to yield Compound III.

Step 3 : The double bond at position 1 on the olefin is epoxidized using standard reagents and solvents, such monoperoxyphthalic acid magnesium salt in isopropanol, to produce the epoxide, Compound IV.

Step 4:

The epoxide is rearranged under acidic conditions, such as aqueous NaHC0 ₃ , using standard techniques to generate the ketone, Compound V.

Step 5;

Alkylation of the bis-alkyl-2-tetrone (Compound V) is accomplished under basic conditions in non-polar solvent using a dihaloalkyl reagent, such as dibromobutane, to yield Compound VI.

Step 6;

Nucleophilic displacement of bromide is accomplished with an appropriate reagent, such as potassium thiacetate to produce Compound VII .

Step 7;

The 3 position of the S-acylated tetralone (Compound VII) is halogenated in a non-polar solvent, such as a mixture of benzene and dry THF, using an appropriate reagent and

12

non-polar solvent such as Bromine in dry THF to generate Compound VIII .

Step 8;

Cyclization is affected under basic conditions using standard reagents and solvents such as lithium bromide and dry THF under Argon, with the addition of a base such as sodium methoxide, generating the polycyclic compound (Compound IX) .

Step 9t

The ketone group of compound IX is converted to an alkyloxime using standard procedures well known in the art affording compound X.

Steps 10 and 11;

Compound X is reduced using a Borane-THF complex. If conducted in THF, a 50:50 mixture of compounds XI and XII is obtained. If the reaction is conducted in diglyme (2- methoxyethyl ether), the amine, (Compound XII) is selectively produced.

Step 12:

Compound XI can be recycled and reduced to the amine using a Borane-THF complex conducted in diglyme to yield Compound XII.

Compounds of the present invention bind to and block activation of the ionotropic NMDA receptor and thereby prevent excessive Ca ⁺² entry into neurons in NMDA-receptor mediated events that are typical of hypoxic and/or ischemic conditions . Excessive entry of calcium into neuronal cells is a prelude to neuronal damage that

13

follows head injury, strokes, and epileptic seizures; is associated with degenerative diseases such as Alzheimer's disease, Huntington's disease, Parkinson's disease and amytrophic lateral sclerosis (ALS) ; and peripheral neurotoxicity involved in lung and other organ injury.

Accordingly the present invention further relates to the use of compounds according to formula (I) for inhibiting NMDA receptor activation as well as for treating and preventing cell damage or cytotoxicity mediated by NMDA receptor activation in a mammal.

The present invention provides compounds that block EAA- induced convulsions in an in vivo rodent model. It has been found that compounds of the present invention block mortality induced by NMDA, AMPA or bicuculline.

It is appreciated that the compounds of the present invention can be modified by one skilled in the art in such a manner as to attach labels such as radioactive labels enabling detection of the compound for use as a radiotracer. The compounds of the present invention may be used as NMDA antagonists in vi tro or ex vivo as in the case of radio-labeling agents, radiotracers for use with Positron Emission Tomography, paramagnetic agents for use in Magnetic Resonance Imaging, and NMDA receptor-linked calcium channel antagonists.

It is appreciated that the compounds of the present invention can be modified by one skilled in the art in such a manner as to prevent access into the central nervous system such that they can function as NMDA receptor antagonists in peripheral tissues to protect

14

against and/or minimize cytotoxicity (neurotoxicity) involved in peripheral NMDA receptor mediated events.

The present invention also provides pharmaceutical compositions which comprise a pharmaceutically effective amount of the compounds of this invention, or pharmaceutically acceptable salts thereof, and, optionally a pharmaceutically acceptable carrier or adjuvant. The term "pharmaceutically effective amount" refers to the required amount of compound to be administered to a mammal in order to reduce or inhibit NMDA receptor activation, cell damage, convulsions or symptoms associated with neuro degenerative diseases . The amount will depend on factors such as the particular indication being treated, mode of administration, size of individual being treated etc.

Therapeutic methods of this invention comprise the step of treating patients in a pharmaceutically acceptable manner with those compounds or compositions . Such compositions may be in the form of tablets, capsules, caplets, powders, granules, lozenges, suppositories, reconstitutable powders, or liquid preparations, such aε oral or sterile parenteral solutions or suspensions.

The therapeutic agents of the present invention may be administered alone or in combination with pharmaceutically acceptable carriers . The proportion of each carrier is determined by the solubility and chemical nature of the compound, the route of administration, and standard pharmaceutical practice.

In order to obtain consistency of administration, it is preferred that a composition of the invention is in the form of a unit dose. The unit dose presentation forms for

15

oral administration may be tablets and capsules and may contain conventional excipients. For example, binding agents, such as acacia, gelatin, sorbitol, or polyvinylpyrolidone; fillers, such as lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants such as magnesium stearate; disintegrants, such as starch, polyvinylpyrrolicone, sodium starch blycollate or microcrystalline cellulose; or pharmaceutically acceptable wetting agents such as sodium lauryl sulphate.

The compounds may be administered parenterally; this being intramuscularly, intravenously, or subcutaneously. For parenteral administration, the compounds may be used in the form of sterile solutions containing other solutes, for example, sufficient saline or glucose to make the solution isotonic .

The compounds may be administered orally in the form of tablets, capsules, or granules containing suitable excipients such as starch, lactose, white sugar and the like. The compounds may be administered orally in the form of solutions which may contain coloring and/or flavoring agents. The compounds may also be administered sublingually in the form of tracheas or lozenges in which each active ingredient is mixed with sugar or corn syrups, flavoring agents and dyes, and then dehydrated sufficiently to make the mixture suitable for pressing into solid form.

The solid oral compositions may be prepared by conventional methods of blending, filling, tabletting, or the like. Repeated blending operations may be used to distribute the active agent throughout those compositions

16

employing large quantities of fillers . Such operations are, of course, conventional in the art. The tablets may be coated according to methods well known in normal pharmaceutical practice, in particular with an enteric coating.

Oral liquid preparations may be in the form of emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may or may not contain conventional additives . For example suspending agents, such as sorbitol, syrup, methyl cellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminum stearate gel, or hydrogenated edible fats; emulsifying agents, such as sorbitan monooleate or acaci; non-aqueous vehicles (which may include edible oils) , such as almond oil, fractionated coconut oil, oily esters selected from the group consisting of lycerine, propylene glycol, ethylene glycol, and ethyl alcohol; preservatives, for instance metyl para-hydroxybenzoate, ethyl para- hydroxybenzoate, n-propyl parahydroxybenzoate, or n-butyl parahydroxybenzoate of sorbic acid; and, if desired, conventional flavoring or coloring agents .

For parenteral administration, fluid unit dosage forms may be prepared by utilizing the compound and a sterile vehicle, and, depending on the concentration employed, may be either suspended or dissolved in the vehicle. Once in solution, the compound may be injected and filter sterilized before filling a suitable vial or ampoule and subsequently sealing the carrier or storage package. Adjuvants, such as a local anesthetic, a preservative or a buffering agent, may be dissolved in the vehicle prior to use. Stability of the pharmaceutical composition may be

17

enhanced by freezing the composition after filling the vial and removing the water under vacuum, (e.g., freeze drying the composition) . Parenteral suspensions may be prepared in substantially the same manner, except that the compound should be suspended in the vehicle rather than being dissolved, and, further, sterilization is not achievable by filtration. The compound may be sterilized, however, by exposing it to ethylene oxide before suspending it in the sterile vehicle. A surfactant or wetting solution may be advantageously included in the composition to facilitate uniform distribution of the compound.

The pharmaceutical compositions of this invention comprise a pharmaceutically effective amount of a compound of this invention and a pharmaceutically acceptable carrier. Typically, they contain from about 0.1% to about 99% by weight, preferably from about 10% to about 60% by weight, of a compound of this invention, depending on which method of administration is employed.

The present invention also provides a method for treatment of NMDA receptor mediated cytotoxicity and/or disease in patients, such as mammals, including humans, which comprises the step of administering to the patient a pharmaceutically effective amount of a compound, a pharmaceutically acceptable salt thereof, or a pharmaceutical composition as described above.

Physicians will determine the dosage of the present therapeutic agents which will be most suitable. Dosages may vary with the mode of administration and the particular compound chosen. In addition, the dosage may vary with the particular patient under treatment. The

18

dosage of the compound used in the treatment will vary, depending on the seriousness of the disorder, the weight of the patient, the relative efficacy of the compound and the judgment of the treating physician. Such therapy may extend for several weeks, in an intermittent or uninterrupted manner, until the patient's symptoms are eliminated.

To further assist in understanding the present invention, the following non-limiting examples are provided.

EXAMPLE 1 preparation of 1, 2-dihydro-7-methoxy-4- methylnapthalene

7-methoxy-l-tetralone (25g) was dried via azeotropic distillation of toluene and dissolved in dried THF (200 ml) . The solution was cooled to -70°C (under Ar and methyl magnesium chloride (1.4 m in toluene/THF, 187.5 ml) was added. The combined reaction mixture was allowed to stir at ambient temperature overnight. It was carefully treated with aqueous saturated NHC1 and extracted with ethylacetate. The latter solution was washed with brine, dried over MgS0 ₄ and evaporated. The residue was dissolved in benzene (150ml), p-TSOH (O.lg) was added and the

19

mixture was heated to reflux using a Dean-Stark condenser until the dehydration reaction was complete. This benzene solution was diluted with ethylacetate, washed with NaHC0 ₃ , dried over MgS0 ₄ and evaporated. The residue was extracted with hexases, passed through a silica gel column and eluted with a mixture of hexases and ethylacetate (1:0, 400:1, 200:1) . The yield of the product was 20.87g (84.42%) .

^X H NMR (300 MHz, CDC1 ₃ ) δ: 2.05 (s, 3H) ; 2.23 (m, 2H) ; 2.69 (t, 2H) ; 3.81 (ε, 3H) 5.88 (m, IH) ; 6.68 - 7.06 ( , 3H) .

EXAMPLE 2 preparation of 7- methoxy -1- methyl -2- tetralone

Dihydro -7- methoxy -4- methylnapthalene (20.87 g) was dissolved in isopropanol (100 ml) and cooled in an ice bath. Monoperoxyphthaiic acid magnesium εalt (mmpp) (17 g) was added, then water (50 ml) was added and the mixture was stirred at room temperature for 2 hours. When oxidation was complete, the product mixture was hydrolyzed with aqueous NaHC0 ₃ , partially evaporated and extracted with ethylacetate. The latter extract was washed with brine and evaporated. The residue was dissolved in a mixture of ethanol (156 ml), water (121 ml) and cone. H ₂ S0 ₄

20

(24.3 ml), and heated to reflux under N ₂ atmosphere for 3 hours, cooled and neutralized with NaHC0 ₃ . After partial evaporation, the residue was extracted with ethylacetate, washed with brine, dried over MgS0 ₄ and evaporated. The product was purified on a silica gel column using a mixture of hexases and ethylacetate (100:1, 50:1, 50:1.5) . The yield of the product was 16.2 g (71%) .

^■■ Η NMR (300 MHz, CDC1 ₃ ) δ: 1.47 (d, 3H) ; 2.55 (m, 2H) ; 3.02 ( , 2H) ; 3.5 (m, IH) ; 3.81 (s, 3H) ; 6.75 - 6.77 (m, 3H) ppm.

IR (film) 1714 cm ^"1

EXAMPLE 3 preparation of 1- (4'- bromobutyl) -1- methyl -7- methoxy -2- tetralone

+ Br (CH ₂ ) ₄ Br + NaHMDS

7-Methoxy -1- methyl -2- tetralone (4 g) was dried via azeotropic diεtillation of toluene, dissolved in dry THF (150 ml), cooled in an ice bath under Ar atmosphere and sodium bis (trimethylsilyl) amide solution (NaHMDS) (1 m in THF, 23.13 ml) was added and stirred for V2 hour. 1,4-

Dibromobutane (9.78 ml) was added and the reaction mixture was allowed to warm up to room temperature overnight, after which it was hydrolyzed with brine, extracted with ethylacetate, dried over MgS0 ₄ and evaporated. The product mixture was purified on a silica gel column using a mixture of hexanes and ethylacetate (200:1, 150:1,100:1,

21

75:1 and 50:11 The yield of the product was 5.19 g (76%) .

¹ H NMR: (300 MHz, DCDl ₃ ) δ: 1.09 (m, 2H) ; 1.37 (s, 3H) ; 1.71 (m, 3H) ; 2.1 (m, 3H) ; 2.68 (m, 2H) ; 2.97 (m, 2H) ; 3.26 (t, 2H) ; 3.8 (s, 3H) , 6.72 -7.09 (m, 3H) ppm.

EXAMPLE 4 preparation of 1- (4'- acetothiobutyl) -1- methyl -7- methoxy -2- tetralone

1- (4' -Bromobutyl) -1- methyl -7- methoxy -2- tetralone

(4.48 g) waε dried via azeotropic distillation of toluene and dissolved in dry DMF (25 ml) . Potassium thiacetate

(5.86 g) was added and the mixture was allowed to stir under Ar atmosphere overnight, after which it was extracted with ethylacetate, washed with brine, dried over MgS0 ₄ and evaporated. The residue was purified on a silica gel column using a mixture of hexanes and ethylacetate

(75:1, 50:1, 20:1) . The yield of the product was 3.9 g

(88.3%) .

^** Η NMR (300 MHz, CDC1 ₃ ) δ: 0.99 (m, 2H) ; 1.5 (m, 6H) ; 2.07 (m, IH) ; 2.25 (s, 3H) ; 2.65 (m, 4H) ; 2.95 (m, 2H) ; 3.79 (s, 3H) ; 6.71 - 7.08 (m, 3H) ppm.

22

EXAMPLE 5 preparation of an epimeric mixture of 3 - bromo -1- (4'- acetothiobutyl) -1- methyl 7- methoxy -2- tetralone

SCOCH, SCOCH,

1- (4'- Acetothiobutyl) -1- methyl -7- methoxy -2- tetralone (4 g) was dried via azeotropic distillation of toluene. It was disεolved in a mixture of benzene (244 ml) and dry THF (64 ml) and stirred at room temperature under Ar atmosphere. Bromine (0.8 ml) was disεolved in dry THF (26 ml) and gradually added to the reaction mixture under Ar flow. After 1 hour of εtirring, the product mixture was hydrolyzed with aqueous NaHC0 ₃ , extracted with ethylacetate, waεhed with brine, dried over MgS0 ₄ and evaporated. The reεidue was dried via azeotropic distillation of toluene and then dried further under high vacuu .

EXAMPLE 6 preparation of 5, 6, 7, 8, 9, 11, 12 - heptahydro -3- methoxy -5- methyl -10- thia -5, 11-ethano benzocyclodecen -13- one

SCOCH,

An epimeric mixture of 3- bromo -1- (4' - acetothiobutyl) -1- methyl -7- methoxy -2- tetralone (approximately 6.25 mmol) was dried via azeotropic distillation of toluene and

23

disεolved in dry THF (200 ml), Lithium Bromide (dry, 0.54 g) waε added, the εolution waε degassed with Ar at room temperature for one hour, and was cooled in an ice bath, well stirred, with a gentle flow of Ar passing through it. Sodium methoxide (0.5 m in Methanol, 13.75 ml) was dissolved in dry THF (75 ml), degasεed with Ar at room temperature for one hour, after which it was added to the latter solution through a εyringe pump over 4 hourε. The combined reaction mixture was stirred for an additional V hour, diluted with ethylacetate (100 ml) , washed with brine, dried over MgS0 ₄ and evaporated. The residue was purified on a silica gel column using a mixture of hexanes and ethylacetate (75:1, 50:1) . The yield of product was approximately 50-55%. It solidified on standing.

^■ " ^■ H NMR (300 MHz, CDC1 ₃ ) δ: 1.4 - 1.95 (m, 4H) ; 2.85 ( ,

2H) ; 2.7 - 3 (m, 2H) ; 3.4 (m, IH) ; 3.82 (m, 4H) ; 6.7 - 7.1

( , 3H) ppm.

IR (film) 1693, 1609 cm ^"

EXAMPLE 7 preparation of epimeric 5, 6, 7, 8, 9, 11, 12 hepta hydro -3- methoxy -5- methyl -10- thia -5, 11 - methanobenzocyclodecen -13- oxime

7, 8, 9, 11, 12 - Heptahydro -3- methoxy -5- methyl -10- thia -5,11- methanobenzocyclodecen -13- one (1.32 g) was dried via azeotropic distillation of toluene, mixed with

24

hydroxyl amine hydrochloride (2.64 g) and dry pyridine (5.2 ml) was added. Combined mixture was heated to 80°C for 2 dayε . It waε cooled, diluted with CH ₂ C1 ₂ and washed with brine. After drying over MgS0 ₄ , the solvent was evaporated off and the residue was purified on a silica gel column using a mixture of hexanes and ethylacetate (50:1, 2501, 10:1, 5:1 8 2:1) . The yield of the product was 1.22g. (92%) .

^*** H NMR (300 MHz, CDCl ₃ ) δ; 1.2 - 1.9 (m, 9H) ; 2.4 (m, 2H) ; 2.85 (m, 2H) ; 3.2 (dd, IH) ; 3.8 (s, 3H) ; 5.11 (t, IH) ; 6.6 - 7.1 (m, 3H) ppm.

IR (film) 1609, 2200, 3250 cm ^"1 Mass Spectrophotometry: m/z 292.

25

EXAMPLE 8 preparation of 5, 6, 7,8, 9, 11, 12 - heptahydro -3- methoxy -5- methyl -10- thia -5,11- methano benzocyclodecen -13- hydroxylamine compound #8a and 5, 6, 7, 8, 9, 11, 12- heptahydro -3- methoxy -5- methyl -10- thia -5,11- methanobenzocyclodecen -13- amine compound #8b

compound #8a compound #8b

7, 8, 11, 12- Heptahydro -3- methoxy -5- methyl -10- thia -5, 11- methanobenzocyclodecen -13- oxime (iεomeric mixture, 0.3 g) was dried with toluene and diεεolved in dry THF (30 ml) . It waε cooled in an ice bath under Ar atmosphere. Borane - THF complex (IM εolution in THF, 7.87 ml) was added and the combined mixture was heated to reflux for 30 hourε. It waε cooled in an ice bath. Water (0.4 ml) and concentrated HCl (0.6 ml) were added carefully in respective order. The mixture was heated to reflux for 15 minuteε, cooled and evaporated. The reεidue was basified with concentrated NH ₄ 0H to pH 12, extracted with CH ₂ C1 ₂ , washed with brine, dried over MgS0 ₄ and evaporated. The residue was purified on a silica gel column using a mixture of hexanes and ethylacetate (50:1, 20:1, 10:1, 5:1, 5:1.5, 2:1, 1:1 and 1:2) . The yield of

26

5, 6, 7, 8, 9, 11, 12 - heptahydro-3- methoxy -5- methyl - 10- thia -5.11- methanobenzocyclodecen -13- hydroxylamine was 0.073 g (23.1%) . It was crystallized from a mixture of ethylacetate and hexanes .

^X H NMR (300 MHz, CDC1 ₃ ) δ: 1.1 - 1.91 (m, 9H) ; 2.3 (m, 2H) ; 3.30 (d, IH) ; 3.37 (m, 2H) ; 3.7 (m, IH) , 3.78 (s, 3H) , 6.6. - 7.1 (m, 3H) ppm.

IR (film) : 1612, 3300 cm ^"1

Mass Spectrometry: 293.8, 275.8, 260.8.

The structure of #8a waε confirmed by singlecrystal X-ray crystallography.

The yield of 5, 6, 7, 8, 9, 11, 12- heptahydro -3- methoxy -5- methyl -10- thia - 5, 11- methanobenzocyclodecen -13- amine was 0.0968g. (32%) . The free base was soluble in hexanes.

^■ NMR (300 MHz, CDC1 ₃ ) δ: 0.8-2.5 (m, 11H) , 3.18 (m, 3H) ,

3.6 (q, IH) , 3.8 (s, 3H) 6.6 - 7.1 (m, 3H) ppm.

This product was disεolved in ether (40 ml) and acidified with Methanol-HCl. The suspenεion was allowed to settle and filtered. The precipitate waε washed with ether and dried, yielding 0.090 g of product.

^X K NMR (300 MHz, CDC13) δ: 0.8 - 1.7 (m, 6H) ; 1.8 (m, 2H) ; 2.0 - 2.5 (m, 3H) ; 3.45 (m, 2H) ; 3.5 (m, 2H) ; 3.8 (S, 3H)

6.7 - 7.1 (m, 3H) ppm. Mass Spectrometry: m/z 278.

27

EXAMPLE 9 preparation of 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy -5- methyl -10- thia - 5, 11 methanobenzocyclodecen -13- amine (sulphazocine) compound #9

compound #9

7, 8, 9, 11, 12- Heptahydro -3- methoxy -5- methyl -10- thia - 5,11- methanobenzocyclodecen -13- amine (0.260 g) was dried via azeotropic diεtillation of toluene and dissolved in dry CH ₂ C1 ₂ (40 ml) . It was cooled to -70° under Ar atmosphere. Boron tribromide solution (IM solution in CH ₂ C1 ₂ , 187 ml) was added and the combined mixture was allowed to stir at ambient temperature overnight. The reaction mixture was hydrolyzed with NaHC0 ₃ , the pH lowered with NH ₄ OH to 12, and extracted with CH ₂ C1 ₂ . The latter solution was dried over MgS0 ₄ and evaporated. The residue was purified on a silica gel column using a mixture of toluene and ethylacetate. (10:1, 5:1, 2:1, 1:1, 1:2) .

The yield of 5, 6, 7, 8, 9, 11, 12- heptahydro -3- hydroxy- 5- methyl-10- thia -5, 11- methane benzocyclodecen -13- amine was 0.162g (65%) .

^** Η NMR (350 Mhz, DMS0-D ₆ ) δ: 1.02 (m, IH) ; 1.25 (m, 5H) ; 1.55 (m 2H) ; 2.01 ( , 2H) ; 2.55 (m, IH) ; 2.97 (d, IH) 3.08 (m, IH) , 3.14 (m, 2H) ; 6.4 - 6.9 (m, 3H) ppm.

28

The above product was converted to its hydrochloride salt and purified via HPLC.

EXAMPLE 10 preparation of 5, 6, 7, 8, 9, 11, 12 - hepta hydro -3- hydroxy -5- methyl -10- thia -5, 11- methanobenzocyclodecen -13- hydroxylamine compound #10

compound #8a compound #10

8, 9 11, 12 - heptahydro -3- methoxy -5- methy -10- thia - 5, 11- methanobenzoxyclodecen -13- hydroxyl amine (0.166g) was dissolved in a mixture of acetic acid (4.5ml) and 48% HBr (4.5ml) . It was cooled, neutralized carefully with NaHC0 ₃ and extracted with CH ₂ C1 ₂ . Latter solution was washed with brine, dried over MgS0 ₄ and evaporated. Residue was purified on a silica gel column using a mixture of hexanes and ethylacetate (10:1, 10:1.5, 5:1) . The yield of 5, 6, 7, 8, 9, 11, 12 -heptahydro -3- hydroxy -5- methyl -10- thia - 5, 11 - methanobenzocyclodecen -13- hydroxylamine was 0.035g (22%) .

^X H NMR (300 MHz, CDC1 ₃ ) δ: 1.1-1.91 (m, 9H) ; 2.30 ( , 2H) ; 3.28 (m, IH) , 3.34 (m, 2H) ; 3.7 (m, IH) , 6.6 - 7.0 (m, 3H) ppm.

Above product was converted to its hydrochloride salt and purified by HPLC.

29

EXAMPLE 11 5, 6, 7, 8, 9, 11, 12 -heptahydro -5- methyl -5, 11- methanobenzoyclodecene -13- amine -•- HCl- Compound #13

Compound #13 was obtained from Astra, Sweden(CAS 53648-55- 8) (J.W. Downing, Brit J. Anaesth . , 1981, 53, 59; see also Freed, M.E. , et al. , J. Med. Chem. , 16:595-599, 1973) .

EXAMPLE 12 preparation of compound #9a (-)-trans- 5,6,7,8,9,11,12-heptahydro-10-thia-3- hydroxy-5-methyl-5, li¬ methanobenzocyclodecen-13-amine

Compound #9 (2.96g) was mixed with D-tartaric acid (2.01g) disεolved in boiling ethanol (95%, 50mL) and filtered. The insoluble masε was washed with hot ethanol (25mL) . Combined filtrateε were evaporated to dryneεs and the residue was rediεεolved in hot ethanol (20mL) . A fluffy εolid maεε was collected and redisεolved in hot ethanol (15mL) . Cryεtallization was allowed to proceed undisturbed at room temperature for 2 days. Semicrystalline mass was further subjected to similar fractional crystallization process two more times. A εample at thiε stage was found to have a diasteromeric purity of 98% via chiral derivatization with Marfey's reagent. (0.267g)

The tartrate εalt of the title compound (O.lg) waε dissolved in hot methanol (20mL) and transferred to a column packed with Amberlite IRA-400 (Cl ^" form) ion exchange resin (5g, washed succeεsively with methanol, water, 0.IM HCl, water and methanol) . The column was waεhed with methanol (lOOmL) and water (lOOmL) in

30

succession. Combined eluents were evaporated off and lyophilized. Residue (0.076g)

EXAMPLE 13 Preparation of compound #9b (+)-trans- 5,6,7,8,9,11, 12-heptahydro-10-thia-3- hydroxy-5-methyl-5, li¬ methanobenzocyclodecen-13amine

Compound #9-D-tartrate salt (highly enriched in dextrorotatory diastereomer 1.5g) waε mixed with NH0H (lOmL) saturated with sodium chloride and extracted with methylene chloride. The latter was washed with brine, dried over MgS0 ₄ and evaporated. Residue (1.27g) was mixed with L-tartaric acid (0.87g) and boiled with ethanol (95%, lOOmL) filtered, the filtrate then being allowed to crystallize at room temperature for 2 dayε. The precipitated maεε waε allowed to crystallize slowly from hot isopropanol. A sample was found to have a diastereomeric purity of 97% via chiral derivatization with Marfey ^{^} s reagent to give 0.1515g yield.

The tartrate salt of the title compound (0.076g) was disεolved in hot methanol (25mL) and tranεferred to a column packed with activated Amberlite IRA-400 (Cl ^" form,5g) which waε then waεhed with methanol (lOOmL) and water (lOOmL) successively. Combined filtrates were evaporated and lyophilized. reεidue (0.058g)

31

EXAMPLE 14 Preparation of compound #11 trans- 5,6,7,8,9, 11, 12-heptahydro-10-thia-3- hydroxy-5-methyl-5, li¬ methanobenzocyclodecen-13-guanidine

compound #9 compound #11

Compound #9 (0.35g) was dried via azeotropic distillation with toluene and disεolved in dry pyridine (5mL) . 1H- pyrazole-1-carboxamidine hydrochloride (1.29g) and diiεopropyl ethylamine (1.74 mL) waε added. Combined mixture waε heated to 80°C under nitrogen atmosphere for 4 days. Solvent was evaporated off and the residue was purified on a silica gel column using a mixture of methylene chloride and methanol. The product (0.41g) was dissolved in methanol saturated with hydrogen chloride

(5mL) and the solvent evaporated off. The residue was purified by HPLC yielding 0.045g final product.

32

EXAMPLE 15 preparation of compound #12 trans- 5, 6, 7, 8, 9, 11, 12-heptahydro-10-sulphono-3- hydroxy-5-methyl-5, 11-methanobenzocyclo- decen-13-amine

EtOH aq MMPP

compound #12

Compound 9 (O.lg) was dried via azeotropic distillation of toluene, dissolved in dry methylene chloride (20mL) and cooled in an ice bath under Ar atmosphere. Trifluoroacetic anhydride (0.54mL) and pyridine (0.5mL) were added. After εtirring at room temperature overnight the reaction mixture waε hydrolyzed with agueous solution of sodium bicarbonate, extracted with methylene chloride, washed with brine, dried over MgS0 ₄ and evaporated. Reεidue was purified on a εilica gel column using a mixture of hexanes and methylene chloride to yield 0.068g of trans-5, 6,7,8,9, 11, 12-heptahydro-10-thia-3-hydroxy-5- methyl-5, ll-methanobenzocyclodecen-13-trifluoroacetamide.

Trans-5, 6, 7, 8, 9, 11, 12-heptahydro-10-thia-3-hydroxy-5- methyl-5, ll-methanobenzocyclodecen-13-trifluoroacetamide was dissolved in a mixture of ethanol (2mL) and water

33

(ImL) and then cooled in an ice bath. Monoperoxyphthalic acid, magnesium salt hexahydrate (0.21g) was added. After 1 hour aqueous saturated sodium bicarbonate (5mL) was added. Combined mixture was stirred at room temperature overnight and evaporated off and the residue extracted with methylene chloride. The latter solution was washed with brine and evaporated yielding 0.123g trans- 5, 6, 7, 8, , 11, 12-heptahydro-10-sulphono-3-hydroxy-5-methyl- 5, ll-methanobenzocyclodecen-13-trifluoroacetamide.

Trans-5, 6, 7, 8, , 11,12-heptahydro-10-sulphono-3-hydroxy-5- methyl-5, ll-methanobenzocyclodecen-13-trifluoroacetamide (0.123g) was dried via azeotropic distillation with toluene and anhydrous hydrazine (2mL) was added. The mixture was stirred at room temperature for 2 days then evaporated dried under vacuum. The product mixture was dissolved in methanol (2mL) and allowed to stand at room temperature. Crystalline material (0.037g) was filtered out and dissolved in a saturated solution of hydrogen chloride in methanol (2mL) and then evaporated. The residue was lyophilized yielding 0.031g of the title compound.

EXAMPLE 16 time course of the neuroprotective activity of the compounds of the invention

For purposes of comparison, the compounds of the present invention and reference standards were administered to mice and the time course of neuroprotectant activity was determined subsequent to challenge by NMDA. Compounds were administered i.e.v. or i.p. at different times and concentrations prior to injection of NMDA (1 nmol/mouse, I.C.V.) . The mortality rate (CD ₅₀ ) TABLE I and the

34

response curves.

The results are presented in Figures 1, 2 and 3. Figure 1 depicts a dose dependent neuroprotectant activity effect of dextrorphan following NMDA challenge in mice. The results demonstrate the time-dependent decreases in the protecting effects of the drug at various doses. In Figure 1, dextrorphan was i.e.v. preadministered at a concentration of 1.0 (closed circle); 5.0 (open square); and 10 (closed square) nmole/mouse. The open circle represents preadministration of saline at a concentration of lOμl/mouse.

A time course profile of the neuroprotectant activity of compound #13 in mice is presented in Figure 2. The figure illustrates time-dependent decreases in the protecting effects of i.e.v. preadministration of compound #13 at varying doses against NMDA. A does dependent time course profile of the neuroprotectant activity against NMDA induced convulsions of compound #9 in mice is illustrated in Figure 3. The graph illustrates time-dependent decreases in the protective effects of Compound #9 at various doses. In both Figures 2 and 3, the respective compound was preadministered at 25 (open circle) ; 50 (closed circle) ; and 100 (closed square) nmo1e/raouse.

35

RECTIFIED SHEET (RULE 91)

TABLE I

Comparison of anticonvulsant activity of compounds of the present invention and dextrorphan against NMDA-induced convulsions in mice (at 1 nmole/mouse)

CDso

Compound nmol/mouse, icv

(95% confidence interval)

Dextrorphan 0.55 (0.39 - 0.78)

Compound #8b 7.64 (4.8 - 12.0)

Compound #9 0.95 (0.62 - 1.45)

Compound #9a 2.90 (1.75 - 4.85)

Compound #9b 1.58 (0.95 - 2.62)

Compound #10 1.79 (0.99 - 3.25)

Compound #11 0.16 (0.11 - 0.25)

Compound #13 28.7 (15.3 - 53.8)

Compounds of the invention demonstrated anticonvulsive activity againεt NMDA-induced convulsions in mice. Compound #11 demonstrated the greatest activity, posεessing approximately 3 fold greater potency than Dextrorphan.

EXAMPLE 17 MOTOR IMPAIRMENT

A. Locomotion and Falling Behaviour

Mice were place individually in obεervation cages for a 60 minute habituation period. They were injected I.C.V. with test compounds and observed for 15 to 30 minutes. Locomotion and falling behavior were assessed according to

36

the procedure of Koek and Colpaert (J. Pharm. Exp. Therm. 252 349-357, 1990) . For each animal, the presence of locomotor activity and falling behaviour was recorded. Statistical significance of drug-induced changes in the occurrence of a particular behaviour was tested by means of the method of Fray et al. (Psycopharmacol 69_ 253-259, 1980) . MK-801, known to have intrinsic neurotoxicity, induced falling behaviour at doses as low as 1.5 nmol/mouse.

B. Assessment of Motor Coordination by Rotarod Motor effects illicited by various antagonists were assessed using a rotarod treadmill (model 7600, UGO Basile, Italy) . The method used was similar to the procedure described by Dunhamand Miya (J. Am. Pharmac. Assoc. 46 208-209- 1957). The apparatus consisted of a rod of 2.5 cm diameter which was suspended horizontally 50cm above a working area. The rod was turned at a speed of 8 rpm. Circular perplex separators were placed at intervals along the rod so that five animals could be tested simultaneously. All animals were conditioned to the rod for two consecutive days prior to experimentation.

The results of a study in which comparative motor iπpairment caused by Cora-pound #13 was compared to dextrorphan, as assessed by rotarod. The percentage of animals showing motor effects is higher for animals treated with destrorphan than for animals treated with Compound #13.

37

RECTIFIED SHEET (RULE 91)