Title:
POLYMERASE INHIBITOR AND METHOD OF USING SAME
Document Type and Number:
WIPO Patent Application WO2004090153
Kind Code:
A3
Abstract:
The present invention provides nucleic acid based polymerase inhibitors and methods for reducing non-specific polymerase extension and amplification in nucleic acid amplification reactions. The polymerase inhibitors provide a double stranded nucleic acid portion that is recognized by a polymerase enzyme as a template for extension but is incapable of being extended by the polymerase enzyme. The polymerase binds to the polymerase inhibitor which sequesters the enzyme until the temperature achieves a level that denatures the double stranded portion of the inhibitor after which the polymerase is released and can then catalyze nucleic acid extension.
Inventors:
MOSER MICHAEL J (US)
VAN HOUT CRISTOPHER V (US)
LARSEN CHRISTINE A (US)
MARSHALL DAVID J (US)
PRUDENT JAMES R (US)
VAN HOUT CRISTOPHER V (US)
LARSEN CHRISTINE A (US)
MARSHALL DAVID J (US)
PRUDENT JAMES R (US)
Application Number:
PCT/US2004/010029
Publication Date:
June 23, 2005
Filing Date:
April 01, 2004
Export Citation:
Assignee:
ERAGEN BIOSCIENCES INC (US)
MOSER MICHAEL J (US)
VAN HOUT CRISTOPHER V (US)
LARSEN CHRISTINE A (US)
MARSHALL DAVID J (US)
PRUDENT JAMES R (US)
MOSER MICHAEL J (US)
VAN HOUT CRISTOPHER V (US)
LARSEN CHRISTINE A (US)
MARSHALL DAVID J (US)
PRUDENT JAMES R (US)
International Classes:
C07H21/04; C12N15/11; C12Q1/48; C12Q; (IPC1-7): C07H21/04; C12N15/11
Domestic Patent References:
| WO2003046149A2 | 2003-06-05 |
Other References:
See also references of EP 1615942A4
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