Title:
POLYNUCLEOTIDE AND EXPRESSION CASSETTE COMPRISING CODING SEQUENCE OF TOLUENE DIOXYGENASE, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/148351
Kind Code:
A1
Abstract:
The present invention relates to the field of bioengineering and medical chemical production. In particular, provided is a polynucleotide comprising a coding sequence of toluene dioxygenase. Also provided are an expression cassette, a vector and a host cell which comprise the polynucleotide, and the use thereof in the preparation of cis-cyclohexadiene-o-diol compounds.
Inventors:
YANG YONG (CN)
HUANG PING (CN)
JIANG BIAO (CN)
ZHU WENFENG (CN)
HUANG PING (CN)
JIANG BIAO (CN)
ZHU WENFENG (CN)
Application Number:
PCT/CN2022/070188
Publication Date:
July 14, 2022
Filing Date:
January 05, 2022
Export Citation:
Assignee:
SHANGHAI SHENGPING MEDICAL EQUIPMENT CO LTD (CN)
SHANGHAI VASTPRO TECH DEVELOPMENT CO LTD (CN)
SHANGHAI VASTPRO TECH DEVELOPMENT CO LTD (CN)
International Classes:
C12N15/53; C12N1/21; C12N9/02; C12N15/72; C12P7/02; C12P7/62; C12P17/18; C12R1/19
Domestic Patent References:
| WO2016053648A1 | 2016-04-07 |
Foreign References:
| US20040033574A1 | 2004-02-19 | |||
| CN202110015598A | 2021-01-06 |
Other References:
DATABASE NUCLEOTIDE 26 April 1993 (1993-04-26), ANONYMOUS : "P.putida toluene dioxygenase (iron-sulfur protein, ferredoxin, reductase), cis-toluene dihydrodiol dehydrogenase, and 3-methylcatechol 2,3-dioxygenase genes, complete cds", XP055949689, retrieved from NCBI Database accession no. J04996
ZYLSTRA, G.J. ET AL.: "Toluene Degradation by Pseudomonas putida F1 Nucleotide Sequence of the TodC1C2BADE Genes and Their Expression in Escherichia coli", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 25, 5 September 1989 (1989-09-05), XP002470166
GIBSON, D.T. ET AL.: "Oxidative Degradation of Aromatic Hydrocarbons by Microorganisms. I. Enzymatic Formation of Catechol from Benzene", BIOCHEMISTRY, vol. 7, no. 7, 31 July 1968 (1968-07-31), XP001320444
ENDOMA MARY ANN, BUI VU P., HANSEN JEFF, HUDLICKY TOMAS: "Medium-Scale Preparation of Useful Metabolites of Aromatic Compounds via Whole-Cell Fermentation with Recombinant Organisms", ORGANIC PROCESS RESEARCH & DEVELOPMENT, AMERICAN CHEMICAL SOCIETY, US, vol. 6, no. 4, 1 July 2002 (2002-07-01), US , pages 525 - 532, XP055949692, ISSN: 1083-6160, DOI: 10.1021/op020013s
BAIDILOV, D. ET AL.: "Chemoenzymatic Synthesis of Advanced Intermediates for Formal Total Syntheses of Tetrodotoxin", ANGEW. CHEM., vol. 130, 30 May 2018 (2018-05-30), XP071375561, DOI: 10.1002/ange.201804602
GIBSON, D. T. ET AL., BIOCHEMISTRY, vol. 7, 1968, pages 2653
BAIDILOV, D ET AL., ANGEW.CHEM. INT. ED, vol. 57, 2018, pages 10994 - 10998
CAS, no. 131043-51-1
"GenBank", Database accession no. 620-4210
ENDOMA, M. A. ET AL., ORGANIC PROCESS RESEARCH & DEVELOPMENT, vol. 6, no. 4, 2002, pages 5250 - 532
SAMBROOK, J ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
BAIDILOV, D. ET AL., ANGEW.CHEM. INT. ED., vol. 57, 2018, pages 10994 - 10998
ZYLSTRA, G.J. ET AL.: "Toluene Degradation by Pseudomonas putida F1 Nucleotide Sequence of the TodC1C2BADE Genes and Their Expression in Escherichia coli", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 25, 5 September 1989 (1989-09-05), XP002470166
GIBSON, D.T. ET AL.: "Oxidative Degradation of Aromatic Hydrocarbons by Microorganisms. I. Enzymatic Formation of Catechol from Benzene", BIOCHEMISTRY, vol. 7, no. 7, 31 July 1968 (1968-07-31), XP001320444
ENDOMA MARY ANN, BUI VU P., HANSEN JEFF, HUDLICKY TOMAS: "Medium-Scale Preparation of Useful Metabolites of Aromatic Compounds via Whole-Cell Fermentation with Recombinant Organisms", ORGANIC PROCESS RESEARCH & DEVELOPMENT, AMERICAN CHEMICAL SOCIETY, US, vol. 6, no. 4, 1 July 2002 (2002-07-01), US , pages 525 - 532, XP055949692, ISSN: 1083-6160, DOI: 10.1021/op020013s
BAIDILOV, D. ET AL.: "Chemoenzymatic Synthesis of Advanced Intermediates for Formal Total Syntheses of Tetrodotoxin", ANGEW. CHEM., vol. 130, 30 May 2018 (2018-05-30), XP071375561, DOI: 10.1002/ange.201804602
GIBSON, D. T. ET AL., BIOCHEMISTRY, vol. 7, 1968, pages 2653
BAIDILOV, D ET AL., ANGEW.CHEM. INT. ED, vol. 57, 2018, pages 10994 - 10998
CAS, no. 131043-51-1
"GenBank", Database accession no. 620-4210
ENDOMA, M. A. ET AL., ORGANIC PROCESS RESEARCH & DEVELOPMENT, vol. 6, no. 4, 2002, pages 5250 - 532
SAMBROOK, J ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
BAIDILOV, D. ET AL., ANGEW.CHEM. INT. ED., vol. 57, 2018, pages 10994 - 10998
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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