Title:
POLYNUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO2000066605
Kind Code:
A3
Abstract:
Provided is a polynucleotide comprising mRNA, rRNA or viral RNA, greater than 25 % of the ribose rings of which are covalently modified at the 2' - OH position. Further provided is a method for producing a double-stranded oligo- or polynucleotide from a template, which comprises contacting the template with a plurality of mononucleotides comprising UTP, dTTP and/or dUTP, ATP and/or dATP, GTP and/or dGTP, and CTP and/or dCTP, in the presence of a nucleic acid polymerase and optionally a template primer under conditions to polymerise the mononucleotides to form a nucleic acid strand complementary to the template, wherein the template comprises an oligo- or polyribonucleotide, a proportion of the ribose rings of which are covalently modified at the 2' - OH position to bear a substituent which enables replication of the template by the nucleic acid polymerase. Also provided is use of a polynucleotide comprising mRNA, rRNA or viral RNA, a proportion of the ribose rings of which are covalently modified at the 2' - OH position, in a hybridisation reaction.
Inventors:
GOLDSBOROUGH ANDREW SIMON (FR)
Application Number:
PCT/GB2000/001687
Publication Date:
April 26, 2001
Filing Date:
May 02, 2000
Export Citation:
Assignee:
CYCLOPS GENOME SCIENCES LTD (GB)
GOLDSBOROUGH ANDREW SIMON (FR)
GOLDSBOROUGH ANDREW SIMON (FR)
International Classes:
C07H21/00; C12P19/34; C12Q1/68; (IPC1-7): C07H21/00; C12Q1/68; C12P19/34
Domestic Patent References:
| WO1992003568A1 | 1992-03-05 | |||
| WO1998013526A1 | 1998-04-02 | |||
| WO1999055857A2 | 1999-11-04 |
Foreign References:
| US5660985A | 1997-08-26 | |||
| US5859221A | 1999-01-12 | |||
| US5872232A | 1999-02-16 | |||
| DE19801153A1 | 1999-07-15 |
Other References:
W XIN ET AL: "Treatment of Duck Hepatitis B Virus by Antisense Poly-2'-O-(2,4-Dinittrophenyl)-Oligoribonucleotides", ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT,MARY ANN LIEBERT, INC., NEW YORK,US, vol. 8, 1998, pages 459 - 468, XP002134080, ISSN: 1087-2906
S YU OVODOV ET AL: "mRNA Acetylated at the 2'-OH Groups of Ribose Residues is Functionally Active in the cell-free Translation System from Wheat Embryos", FEBS LETTERS,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 270, no. 1/2, September 1990 (1990-09-01), pages 111 - 114, XP002134079, ISSN: 0014-5793
SPROAT B S: "Chemistry and applications of oligonucleotide analogues", JOURNAL OF BIOTECHNOLOGY,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 41, no. 2, 31 July 1995 (1995-07-31), pages 221 - 238, XP004036938, ISSN: 0168-1656
N C PAGRATIS ET AL: "Potent 2'-Amino- and 2'-Fluoro-2'-deoxyribonucleotide RNA Inhibitors of Keratinocyte Growth Factor", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 15, no. 1, 1997, pages 68 - 73, XP002134081, ISSN: 1087-0156
HEIDENREICH O ET AL: "CHEMICALLY MODIFIED RNA: APPROACHES AND APPLICATIONS", FASEB JOURNAL,US,FED. OF AMERICAN SOC. FOR EXPERIMENTAL BIOLOGY, BETHESDA, MD, vol. 7, no. 1, 1993, pages 90 - 96, XP002043382, ISSN: 0892-6638
HEIDENREICH ET AL: "High activity and stability of hammerhead ribozymes containing 2'-modified pyrimidine nucleosides and phosphorothioates", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 269, no. 3, 21 January 1994 (1994-01-21), pages 2131 - 2138, XP002096819, ISSN: 0021-9258
AURUP ET AL: "Translation of 2'-modified mRNA in vitro and in vivo", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 23, 1994, pages 4963 - 4968, XP002096818, ISSN: 0305-1048
S YU OVODOV ET AL: "mRNA Acetylated at the 2'-OH Groups of Ribose Residues is Functionally Active in the cell-free Translation System from Wheat Embryos", FEBS LETTERS,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 270, no. 1/2, September 1990 (1990-09-01), pages 111 - 114, XP002134079, ISSN: 0014-5793
SPROAT B S: "Chemistry and applications of oligonucleotide analogues", JOURNAL OF BIOTECHNOLOGY,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 41, no. 2, 31 July 1995 (1995-07-31), pages 221 - 238, XP004036938, ISSN: 0168-1656
N C PAGRATIS ET AL: "Potent 2'-Amino- and 2'-Fluoro-2'-deoxyribonucleotide RNA Inhibitors of Keratinocyte Growth Factor", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 15, no. 1, 1997, pages 68 - 73, XP002134081, ISSN: 1087-0156
HEIDENREICH O ET AL: "CHEMICALLY MODIFIED RNA: APPROACHES AND APPLICATIONS", FASEB JOURNAL,US,FED. OF AMERICAN SOC. FOR EXPERIMENTAL BIOLOGY, BETHESDA, MD, vol. 7, no. 1, 1993, pages 90 - 96, XP002043382, ISSN: 0892-6638
HEIDENREICH ET AL: "High activity and stability of hammerhead ribozymes containing 2'-modified pyrimidine nucleosides and phosphorothioates", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 269, no. 3, 21 January 1994 (1994-01-21), pages 2131 - 2138, XP002096819, ISSN: 0021-9258
AURUP ET AL: "Translation of 2'-modified mRNA in vitro and in vivo", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 23, 1994, pages 4963 - 4968, XP002096818, ISSN: 0305-1048
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