Title:
POLYPEPTIDE ANTIGENS OF MYCOBACTERIUM BOVIS
Document Type and Number:
WIPO Patent Application WO1994002508
Kind Code:
A3
Abstract:
The invention provides a synthetic polypeptide having at least one antigenic property of the MPB-70 protein of at least one strain of (Mycobacterium bovis), said polypeptide of Formula (I) comprising an amino acid sequence selected from Formulae Seq. ID Nos 1-14: Seq. ID No 1: X-Glu-Tyr-Ala-Ala-Ala-Asn-Pro-Thr-Gly-Pro-Ala-Ser-Val-Gln-Gly-Y; Seq. ID No 2: X-Met-Ser-Gln-Asp-Pro-Val-Ala-Val-Ala-Ala-Ser-Asn-Asn-Pro-Y; Seq. ID No 3: X-Pro-Glu-Leu-Thr-Thr-Leu-Thr-Ala-Ala-Leu-Ser-Gly-Gln-Leu-Asn-Pro-Gln-Y; Seq. ID No 4: X-Val-Val-Ala-Gly-Gln-Thr-Ser-Pro-Ala-Asn-Val-Y; Seq. ID No 5: X-Pro-Gln-Val-Asn-Leu-Val-Asp-Thr-Leu-Asn-Ser-Gly-Gln-Tyr-Ser-Val-Y; Seq. ID No 6: X-Phe-Ala-Pro-Thr-Asn-Ala-Ala-Phe-Ser-Y; Seq. ID No 7: X-Pro-Ala-Ser-Thr-Leu-Asp-Glu-Leu-Thr-Asn-Y; Seq. ID No 8: X-Gly-Thr-Arg-Thr-Gln-Leu-Gln-Gly-Ala-Ser-Val-Thr-Y; Seq. ID No 9: X-Gly-Thr-Arg-Gln-Thr-Leu-Gln-Gly-Ala-Ser-Val-Thr-Y; Seq. ID No 10: X-Pro-Ala-Asn-Val-Val-Gly-Thr-Arg-Thr-Gln-Leu-Gln-Gly-Y; Seq. ID No 11: X-Pro-Ala-Asn-Val-Val-Gly-Thr-Arg-Gln-Thr-Leu-Gln-Gly-Y; Seq. ID No 12: X-Thr-Gly-Gln-Gly-Asn-Ser-Leu-Lys-Val-Gly-Asn-Ala-Asp-Y; Seq. ID No 13: X-Ala-Thr-Val-Tyr-Met-Ile-Asp-Ser-Val-Leu-Met-Pro-Pro-Ala-Y; and Seq. ID No 14: X-Pro-Gln-Val-Asn-Leu-Val-Asp-Thr-Leu-Asn-His-Gly-Gln-Tyr-Ser-Y; wherein X and Y may each independently be absent or independently be one or more amino acid residues, with the proviso that when present they do not provide or form part of an epitope of the MPB-70 protein of at least one strain of (Mycobacterium bovis) which is contiguous with the sequence to which X and Y are attached, X or Y optionally being terminated by a functionalised coupling moiety. The synthetic polypeptides according to the invention may be used to elicit the production of antibodies to the MPB-70 protein.
Inventors:
FISHLEIGH ROBERT VINCENT (GB)
ROBSON BARRY (GB)
GREANEY PAUL JOHN (GB)
ROBSON BARRY (GB)
GREANEY PAUL JOHN (GB)
Application Number:
PCT/GB1993/001527
Publication Date:
June 09, 1994
Filing Date:
July 21, 1993
Export Citation:
Assignee:
PROTEUS MOLECULAR DESIGN (GB)
International Classes:
C07K14/35; C12N15/31; A61K39/00; (IPC1-7): C07K7/06; C07K7/08; C12N15/31; C07K15/00; A61K39/04; G01N33/53
Domestic Patent References:
| WO1989006974A2 | 1989-08-10 | |||
| WO1990009395A1 | 1990-08-23 | |||
| WO1989009261A1 | 1989-10-05 |
Other References:
A.J.RADFORD ET AL: "Epitope mapping of the M.bovis secretory protein MPB70 using overlapping peptide analysis", J.GEN.MICROBIOL., vol. 136, 1990, pages 265 - 272, XP001206929
B.WEINSTEIN: "CHEMISTRY AND BIOCHEMISTRY OF AMINO ACIDS, PEPTIDES AND PROTEINS;VOL.7", 1983, MARCEL DEKKER INC., NEW YORK
BILLMAN-JACOBE ET AL: "Mapping of the T and B cell epitopes of the M.bovis protein MPB70", IMMUNOL.CELL.BIOL., vol. 68, 1990, pages 359 - 365
B.WEINSTEIN: "CHEMISTRY AND BIOCHEMISTRY OF AMINO ACIDS, PEPTIDES AND PROTEINS;VOL.7", 1983, MARCEL DEKKER INC., NEW YORK
BILLMAN-JACOBE ET AL: "Mapping of the T and B cell epitopes of the M.bovis protein MPB70", IMMUNOL.CELL.BIOL., vol. 68, 1990, pages 359 - 365
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