Title:
POLYPEPTIDE COMPOSITION ANALYSIS METHOD BASED ON COPPER ION MODIFIED MSPA NANOPORES
Document Type and Number:
WIPO Patent Application WO/2023/030146
Kind Code:
A1
Abstract:
Provided is a polypeptide composition analysis method based on M2MspA-N91H nanopores, comprising the following steps: S1: uniformly mixing and reacting exopeptidase with a polypeptide to be tested, and obtaining a hydrolysate; S2: adding the hydrolysate into a nanopore system, where the nanopore system comprises: M2MspA-N91H nanopores, an insulating film, a first medium, and a second medium, wherein the M2MspA-N91H nanopores are embedded in the insulating film, the M2MspA-N91H nanopores provide a channel communicating the first medium and the second medium, and the hydrolysate is added into the first medium; S3: applying a driving force between the first medium and the second medium, free amino acids in the hydrolysate passing through the M2MspA-N91H nanopores and generating electrical signals; S4: performing data analysis on the electrical signals to obtain amino acid type information for the free amino acids.
Inventors:
GENG JIA (CN)
ZHANG MING (CN)
WANG ZICHUN (CN)
WEI YUQUAN (CN)
CHEN SHANCHUAN (CN)
ZHANG MING (CN)
WANG ZICHUN (CN)
WEI YUQUAN (CN)
CHEN SHANCHUAN (CN)
Application Number:
PCT/CN2022/114670
Publication Date:
March 09, 2023
Filing Date:
August 25, 2022
Export Citation:
Assignee:
UNIV SICHUAN (CN)
International Classes:
G01N27/26; G01N33/487; G01N33/68
Domestic Patent References:
| WO2015051378A1 | 2015-04-09 | |||
| WO2014071250A1 | 2014-05-08 |
Foreign References:
| US20170199149A1 | 2017-07-13 | |||
| CN110954445A | 2020-04-03 | |||
| US20120055792A1 | 2012-03-08 | |||
| CN106133513A | 2016-11-16 | |||
| CN105992634A | 2016-10-05 | |||
| CN105801676A | 2016-07-27 |
Other References:
ZHANG XIALIN, DOU LINQIN, ZHANG MING, WANG YU, JIANG XIN, LI XINQIONG, WEI LONG, CHEN YUEJIA, ZHOU CUISONG, GENG JIA: "Real-time sensing of neurotransmitters by functionalized nanopores embedded in a single live cell", MOLECULAR BIOMEDICINE, vol. 2, no. 1, 1 December 2021 (2021-12-01), XP093041675, DOI: 10.1186/s43556-021-00026-3
Attorney, Agent or Firm:
CHONGQING ENZHOU INTELLECTUAL PROPERTY AGENCY (SPECIAL GENERAL PARTNERSHIP) (CN)
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