Title:
POLYPEPTIDE HAVING ENDONUCLEASE ACTIVITY AND PRODUCTION METHOD FOR SAME
Document Type and Number:
WIPO Patent Application WO/2017/170418
Kind Code:
A1
Abstract:
The present invention provides art for producing, from a polypeptide expression system having yeast as a host, a wild-type endonuclease variant polypeptide derived from Serratia marcescens. This polypeptide: (a) includes an amino acid sequence having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO:1 or an amino acid sequence that is the amino acid sequence represented by SEQ ID NO:1 having one or more amino acid residue substitutions, deletions and/or additions, (b) when produced by secretion using a polypeptide expression system having yeast as a host, is produced by secretion as a polypeptide not having an N-linked sugar chain, and (c) has endonuclease activity.
Inventors:
NISHIYAMA TOZO (JP)
Application Number:
PCT/JP2017/012419
Publication Date:
October 05, 2017
Filing Date:
March 27, 2017
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N9/16; C12N1/16; C12N15/09; C12P21/02
Domestic Patent References:
| WO2014131113A1 | 2014-09-04 |
Foreign References:
| JPS63500142A | 1988-01-21 | |||
| JPH06296482A | 1994-10-25 | |||
| JP2010512160A | 2010-04-22 | |||
| CN105985968A | 2016-10-05 |
Other References:
BIEDERMANN, K. ET AL.: "Purification and characterization of a Serratia marcescens nuclease produced by Escherichia coli", CARLSBERG RESEARCH COMMUNICATIONS, vol. 54, no. 1, 1989, pages 17 - 27, XP 001317025
BIEDERMANN, K. ET AL.: "Fermentation studies of the secretion of Serratia marcescens nuclease by Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 56, no. 6, 1990, pages 1833 - 1838, XP 002561838
DATABASE UniProtKB [o] 17 February 2016 (2016-02-17), XP055545547, retrieved from UniProt Database accession no. A0A0T9KWK0
YURUGI-KOBAYASHI, T. ET AL.: "Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 380, no. 2, 2009, pages 271 - 276, XP 026005747
LEE, S.-B. ET AL.: "Expression of the non- glycosylated kringle domain of tissue type plasminogen activator in Pichia and its anti- endothelial cell activity", PROTEIN EXPRESSION AND PURIFICATION, vol. 50, no. 1, 2006, pages 1 - 8, XP 024908852
HOSHIDA, H. ET AL.: "N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable a-amylase in Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 97, no. 12, 2013, pages 5473 - 5482, XP 035328902
BIEDERMANN, K. ET AL.: "Fermentation studies of the secretion of Serratia marcescens nuclease by Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 56, no. 6, 1990, pages 1833 - 1838, XP 002561838
DATABASE UniProtKB [o] 17 February 2016 (2016-02-17), XP055545547, retrieved from UniProt Database accession no. A0A0T9KWK0
YURUGI-KOBAYASHI, T. ET AL.: "Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 380, no. 2, 2009, pages 271 - 276, XP 026005747
LEE, S.-B. ET AL.: "Expression of the non- glycosylated kringle domain of tissue type plasminogen activator in Pichia and its anti- endothelial cell activity", PROTEIN EXPRESSION AND PURIFICATION, vol. 50, no. 1, 2006, pages 1 - 8, XP 024908852
HOSHIDA, H. ET AL.: "N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable a-amylase in Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 97, no. 12, 2013, pages 5473 - 5482, XP 035328902
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
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