Title:
POPTAG PEPTIDE AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/113598
Kind Code:
A3
Abstract:
Proteins and fusion proteins for forming menbraneless droplets in cells are provided. Described herein, is the development of a protein, named PopTag, that drives phase separation when it is part of a chimeric fusion protein. PopTag is engineered from the PopZ protein, found in a-proteobacteria (including Caulobacter crescentus). Despite PopZ being exclusively found in this clade of bacteria, the PopTag can drive protein phase separation in other prokaryotes (e.g., E. coli) and eukaryotes (e.g., human cells).
Inventors:
LASKER KEREN (US)
BOEYNAEMS STEVEN (US)
GITLER AARON DAVID (US)
SHAPIRO LUCY (US)
BOEYNAEMS STEVEN (US)
GITLER AARON DAVID (US)
SHAPIRO LUCY (US)
Application Number:
PCT/US2020/063245
Publication Date:
July 15, 2021
Filing Date:
December 04, 2020
Export Citation:
Assignee:
CHAN ZUCKERBERG BIOHUB INC (US)
UNIV LELAND STANFORD JUNIOR (US)
UNIV LELAND STANFORD JUNIOR (US)
International Classes:
C12N15/62; C07K14/195
Foreign References:
| US20190322980A1 | 2019-10-24 | |||
| US20130017210A1 | 2013-01-17 | |||
| US20190144872A1 | 2019-05-16 |
Other References:
LIM HOONG CHUIN, BERNHARDT THOMAS G.: "A PopZ‐linked apical recruitment assay for studying protein–protein interactions in the bacterial cell envelope", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 112, no. 6, 1 December 2019 (2019-12-01), GB, pages 1757 - 1768, XP055843278, ISSN: 0950-382X, DOI: 10.1111/mmi.14391
MARKS MELISSA E., CASTRO-ROJAS CYD MARIE, TEILING CLOTILDE, DU LEI, KAPATRAL VINAYAK, WALUNAS THERESA L., CROSSON SEAN: "The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus", JOURNAL OF BACTERIOLOGY (PRINT), AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 192, no. 14, 15 July 2010 (2010-07-15), US, pages 3678 - 3688, XP055843283, ISSN: 0021-9193, DOI: 10.1128/JB.00255-10
MARKS MELISSA E., CASTRO-ROJAS CYD MARIE, TEILING CLOTILDE, DU LEI, KAPATRAL VINAYAK, WALUNAS THERESA L., CROSSON SEAN: "The Genetic Basis of Laboratory Adaptation in Caulobacter crescentus", JOURNAL OF BACTERIOLOGY (PRINT), AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 192, no. 14, 15 July 2010 (2010-07-15), US, pages 3678 - 3688, XP055843283, ISSN: 0021-9193, DOI: 10.1128/JB.00255-10
Attorney, Agent or Firm:
HINSCH, Matthew E. et al. (US)
Download PDF:
Previous Patent: METHODS OF TREATMENT USING G-CSF PROTEIN COMPLEX
Next Patent: FLANGED BEARING, ASSEMBLY, AND METHOD OF MAKING AND USING THE SAME
Next Patent: FLANGED BEARING, ASSEMBLY, AND METHOD OF MAKING AND USING THE SAME