Title:
POST-SYNTHESIS LABELING OF NUCLEIC ACIDS AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2003052115
Kind Code:
A3
Abstract:
An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside a-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism.
Inventors:
BRUSH CHARLES K
GUPTA VINEET
GUPTA VINEET
Application Number:
PCT/SE2002/002331
Publication Date:
October 09, 2003
Filing Date:
December 13, 2002
Export Citation:
Assignee:
AMERSHAM BIOSCIENCES AB (SE)
International Classes:
G01N33/53; C12M1/00; C12N15/09; C12Q1/68; C12Q1/6816; C12Q1/6827; C12Q1/6837; G01N33/566; (IPC1-7): C12Q1/68; C07K101/02; C12N15/11
Domestic Patent References:
| WO1990008838A1 | 1990-08-09 | |||
| WO1995015395A2 | 1995-06-08 | |||
| WO1995002422A1 | 1995-01-26 | |||
| WO1995000638A2 | 1995-01-05 |
Foreign References:
| EP0123513A1 | 1984-10-31 |
Other References:
BERG CECILIA ET AL.: "Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides", CLIN. CHEM., vol. 41, no. 10, 1995, pages 1461 - 1466, XP002962558
DATABASE BIOSIS [online] KULKARNI R.P. ET AL.: "Synthesis of T. thermophila tRNA with 5'-terminal phosphorothioate group with subsequent maleimide chemical ligation", XP002962559, Database accession no. PREV200200178937
DATABASE WPI Week 198615, Derwent World Patents Index; Class B04, AN 1986-098370, XP002962562
AUER M. ET AL.: "Synthesis and biological applications of 2',3'-dideoxynucleoside-5'-O-(alpha-thio)triphosphates", NUCLEOSIDES & NUCLEOTIDES, vol. 8, 1989, pages 849 - 853, XP002962563
LATIMER LAURA J.P. ET AL.: "Synthetic repeating sequence DNAs containing phosphorothioates: nuclease sensitivity and triplex formation", NUCLEIC ACIDS RESEARCH, vol. 17, no. 4, 1989, pages 1549 - 1561, XP002962564
ECKSTEIN FRITZ ET AL.: "Polyribonucleotides containing a phosphorothioate backbone", EUR. J. BIOCHEM., vol. 13, 1970, pages 558 - 564, XP002962565
ZOELLER R. THOMAS ET AL.: "N-ethylmaleimide (NEM) can significantly improve in situ hybridization results using 35S-labeled oligodeoxynucleotide or complementary RNA probes", THE JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 45, no. 7, 1997, pages 1035 - 1041, XP002962566
DATABASE BIOSIS [online] ZHAO XIAODONG ET AL.: "Attachment chemistry for manufacturing DNA microarray chips", XP002962567, Database accession no. PREV200100516863
DATABASE BIOSIS [online] KULKARNI R.P. ET AL.: "Synthesis of T. thermophila tRNA with 5'-terminal phosphorothioate group with subsequent maleimide chemical ligation", XP002962559, Database accession no. PREV200200178937
DATABASE WPI Week 198615, Derwent World Patents Index; Class B04, AN 1986-098370, XP002962562
AUER M. ET AL.: "Synthesis and biological applications of 2',3'-dideoxynucleoside-5'-O-(alpha-thio)triphosphates", NUCLEOSIDES & NUCLEOTIDES, vol. 8, 1989, pages 849 - 853, XP002962563
LATIMER LAURA J.P. ET AL.: "Synthetic repeating sequence DNAs containing phosphorothioates: nuclease sensitivity and triplex formation", NUCLEIC ACIDS RESEARCH, vol. 17, no. 4, 1989, pages 1549 - 1561, XP002962564
ECKSTEIN FRITZ ET AL.: "Polyribonucleotides containing a phosphorothioate backbone", EUR. J. BIOCHEM., vol. 13, 1970, pages 558 - 564, XP002962565
ZOELLER R. THOMAS ET AL.: "N-ethylmaleimide (NEM) can significantly improve in situ hybridization results using 35S-labeled oligodeoxynucleotide or complementary RNA probes", THE JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 45, no. 7, 1997, pages 1035 - 1041, XP002962566
DATABASE BIOSIS [online] ZHAO XIAODONG ET AL.: "Attachment chemistry for manufacturing DNA microarray chips", XP002962567, Database accession no. PREV200100516863
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