Title:
PREGABALIN ARTIFICIAL HAPTEN AND PREGABALIN ARTIFICIAL ANTIGEN, PREPARATION METHODS THEREFOR, AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/188302
Kind Code:
A1
Abstract:
Disclosed are a pregabalin artificial hapten and a pregabalin artificial antigen, preparation methods therefor, and applications thereof. The molecular structural formula of the pregabalin artificial hapten is as shown in formula (I), and the molecular structural formula of the pregabalin artificial antigen is as shown in formula (II). The application is an application of the pregabalin artificial antigen in the preparation of an anti-pregabalin antibody. The pregabalin artificial hapten retains the feature structure of pregabalin to the greatest extent, has an active group that can be conjugated to a carrier protein, and thus can be used as an antigenic determinant. The further prepared pregabalin artificial antigen can be immunize to obtain an anti-pregabalin antibody having high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing New Zealand white rabbits is as high as 1:90000, and the anti-pregabalin antibody can be used for rapid and accurate immunoassay and immunoassay of pregabalin.
Inventors:
SHAO YUESHUI (CN)
WANG ZHEN (CN)
WANG ZHEN (CN)
Application Number:
PCT/CN2021/102822
Publication Date:
September 15, 2022
Filing Date:
June 28, 2021
Export Citation:
Assignee:
HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07C233/31; C07C231/02; C07K14/765; C07K16/44; G01N33/53
Domestic Patent References:
| WO2016140799A1 | 2016-09-09 |
Foreign References:
| CN105315363A | 2016-02-10 | |||
| CN106084059A | 2016-11-09 | |||
| CN104447383A | 2015-03-25 | |||
| CN105481859A | 2016-04-13 |
Attorney, Agent or Firm:
KG INTELLECTUAL PROPERTY LAW FIRM (CN)
Download PDF:
Previous Patent: VISIBLE LIGHT SPARSE ARRAY WAVEGUIDE OPTICAL PHASED ARRAY
Next Patent: EARMUFF AND HEADPHONE
Next Patent: EARMUFF AND HEADPHONE