Title:
PREPARATION METHOD FOR AND APPLICATION OF IMMOBILIZED CELL FOR TAGATOSE PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2023/279565
Kind Code:
A1
Abstract:
Provided are a preparation method for an immobilized cell for tagatose production and a method for producing tagatose by using the immobilized cell. The preparation method for the immobilized cell comprises: mixing a fermentation broth of Escherichia coli or Bacillus subtilis that expresses α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, tagatose 6-phosphate epimerase, and tagatose 6-phosphate phosphatase to obtain a fermentation mixture, adding inorganic soil and then performing uniform stirring, then adding a flocculant to flocculate bacteria, subsequently adding a cross-linking agent to cross-link, performing vacuum filtration to obtain a filter cake, using a rotary granulator to extrude the filter cake to granulate into a long strip, then cutting by means of a spherical shot blasting machine into particles having equal lengths, and performing boiling drying to obtain the immobilized cell for tagatose production. According to the present invention, separation and purification steps of an enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is achieved.
Inventors:
MA YANHE (CN)
SHI TING (CN)
HAN PINGPING (CN)
SHI TING (CN)
HAN PINGPING (CN)
Application Number:
PCT/CN2021/123610
Publication Date:
January 12, 2023
Filing Date:
October 13, 2021
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N11/14; C12N11/082; C12N11/087; C12N11/089; C12N11/10; C12P19/02; C12R1/125; C12R1/19
Foreign References:
| CN113249371A | 2021-08-13 | |||
| CN112342179A | 2021-02-09 | |||
| CN104313009A | 2015-01-28 | |||
| CN101532006A | 2009-09-16 | |||
| CN112760317A | 2021-05-07 | |||
| CN112760316A | 2021-05-07 | |||
| CN106399427A | 2017-02-15 | |||
| CN107988286A | 2018-05-04 | |||
| CN112342179B | 2021-04-06 | |||
| CN107988286B | 2021-03-19 |
Other References:
OH D-K: "Tagatose: properties, applications, and biotechnological processes", APP. MICROBIOL. BIOTECHNOL, vol. 76, 2007, pages 1 - 8, XP019538798, DOI: 10.1007/s00253-007-0981-1
SAMBROOK ET AL.: "Molecular Cloning: Laboratory Handbook", 1989, COLD SPRING HARBOR LABORATORY PRESS
SAMBROOK ET AL.: "Molecular Cloning: Laboratory Handbook", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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