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Title:
PREPARATION METHOD FOR AND APPLICATION OF IMMOBILIZED CELLS FOR MANNOSE PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2023/279687
Kind Code:
A1
Abstract:
A method for preparing immobilized cells for producing mannose, and a method using same for producing mannose, comprising: fermenting to separately obtain fermentation broths of Escherichia coli or Bacillus subtilis expressing α-glucan phosphorylase, phosphoglucomutase, glucose phosphoisomerase, mannose-6-phosphate isomerase and mannose-6-phosphate phosphatase, and mixing the fermentation broths to obtain a mixed fermentation broth.

Inventors:
MA YANHE (CN)
SHI TING (CN)
HAN PINGPING (CN)
Application Number:
PCT/CN2022/070091
Publication Date:
January 12, 2023
Filing Date:
January 04, 2022
Export Citation:
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Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N11/14; C12N11/082; C12N11/087; C12N11/089; C12N11/10; C12P19/02; C12R1/125; C12R1/19
Foreign References:
CN113249372A2021-08-13
CN112342179A2021-02-09
CN109750011A2019-05-14
CN101532006A2009-09-16
CN112778358A2021-05-11
US20180346897A12018-12-06
CN109750011A2019-05-14
CN112342179B2021-04-06
Other References:
FAN, S.-P. ET AL.: "High yield production of sugars from deproteinated palm kernel cake under microwave irradiation via dilute sulfuric acid hydrolysis", BIORESOURCE TECHNOLOGY, vol. 153, no. 0, 2014, pages 69 - 78, XP028669145, DOI: 10.1016/j.biortech.2013.11.055
SAARI, PM. HURME: "Process Synthesis Principles in the Chromatographic Separation of Sugars from Biomass Hydrolysates", CHEMICAL ENGINEERING & TECHNOLOGY, vol. 34, no. 2, 2011, pages 282 - 288, XP071791573, DOI: 10.1002/ceat.201000201
KOCKRITZ, A. ET AL.: "earrangement of glucose to mannose catalyzed by polymer-supported Mo catalysts in the liquid phase", APPLIED CATALYSIS A: GENERAL, vol. 334, 2008, pages 112 - 118
PARK, C.S. ET AL.: "Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartii", BIOTECHNOL LETT, vol. 32, no. 9, 2010, pages 1305 - 1309, XP019813482
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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