Title:
PREPARATION METHOD FOR BIONIC MULTI-COMPONENT FIBER CARRYING SEED CELLS, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/055893
Kind Code:
A1
Abstract:
A preparation method for a bionic multi-component fiber carrying seed cells, and an application thereof. The preparation method comprises the following steps: S1, 1) drawing and assembling tubes I with pointed ends; 2) drawing a tube II having a pointed end; and 3) drawing a tube III having an inner diameter greater than those of the tube I and the tube II; and inserting the tube I and the tube II into the tube III through two openings of the tube III, respectively, inserting the pointed ends of the tubes I into a non-pointed end of the tube II, adjusting the axes of the tubes I, the tube II and the tube III to make same coincide, and fixing the tubes I, the tube II and the tube III in place; S2, drawing a tube IV with a pointed end, coaxially nesting the pointed ends of the tube IV and tubes I, and installing needles outside the tubes I, the tube III and the tube IV; and S3, 1) defining each solution channel and preparing solutions; introducing deionized water into an external solution channel, then sequentially and correspondingly introducing a PVA solution, a sodium alginate solution and a calcium chloride aqueous solution, and gradually reducing the flow rate of the deionized water until the deionized water is completely cut off, so as to obtain a calcium alginate fiber; and using a calcium chloride solution to collect the calcium alginate fibers.
Inventors:
ZHAO YUANJIN (CN)
WANG JINGLIN (CN)
REN HAOZHEN (CN)
CHEN GUOPU (CN)
SHANG YIXUAN (CN)
WANG JINGLIN (CN)
REN HAOZHEN (CN)
CHEN GUOPU (CN)
SHANG YIXUAN (CN)
Application Number:
PCT/CN2023/117476
Publication Date:
March 21, 2024
Filing Date:
September 07, 2023
Export Citation:
Assignee:
NANJING DRUM TOWER HOSPITAL (CN)
International Classes:
D01F9/04; C12N5/077; D01F1/10
Foreign References:
| CN115262028A | 2022-11-01 | |||
| CN114790441A | 2022-07-26 | |||
| CN112921436A | 2021-06-08 | |||
| KR100740169B1 | 2007-07-16 | |||
| CN103820880A | 2014-05-28 | |||
| CN106215987A | 2016-12-14 | |||
| CN108360088A | 2018-08-03 | |||
| CN114457442A | 2022-05-10 | |||
| CN114854677A | 2022-08-05 | |||
| JP2013009598A | 2013-01-17 | |||
| JP2014236698A | 2014-12-18 | |||
| JP2016077229A | 2016-05-16 | |||
| US20210340481A1 | 2021-11-04 |
Attorney, Agent or Firm:
NANJING ZHONGSHAN PATENT AGENT CO., LTD. (CN)
Download PDF: