Title:
PREPARATION OF AND METHOD FOR IMMUNOMODULATORY PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2021/082294
Kind Code:
A1
Abstract:
Provided is a method for isolating and purifying an immunomodulatory peptide by using Pseudostellaria heterophylla. The method uses Pseudostellaria heterophylla as a raw material to obtain a specific immunity polypeptide after using protein extraction, the two-step enzymolysis of pepsin and trypsin, isolation and purification, and freeze-drying. The polypeptide has a molecular weight of 522 Da, and the full amino acid sequence thereof is: Arg-Gly-Pro-Pro-Pro. The present invention eliminates the defects present in immunomodulatory drugs, and eliminates public worries about artificial immunomodulators; in addition, the present invention lays the theoretical foundation for the development of immunity peptides based on food sources as well as for the exploration of the wide applications thereof in food and medicine.
More Like This:
| JP2010143936 | ZINC FINGER BINDING DOMAIN FOR GNN |
| JP6707576 | Solid phase peptide synthesis via side chain binding |
| WO/2001/096369 | GLUTHIONE ANALOGUES AND THEIR USE AS ANTIOXIDANTS |
Inventors:
WANG SHAOYUN (CN)
YANG QIAN (CN)
CAI QIANQIAN (CN)
CHEN XU (CN)
TIAN YONGQI (CN)
YANG QIAN (CN)
CAI QIANQIAN (CN)
CHEN XU (CN)
TIAN YONGQI (CN)
Application Number:
PCT/CN2020/073062
Publication Date:
May 06, 2021
Filing Date:
January 19, 2020
Export Citation:
Assignee:
UNIV FU ZHOU (CN)
International Classes:
C07K7/06; C07K1/20; C12P21/06
Foreign References:
| CN110655556A | 2020-01-07 |
Other References:
YANG QIAN, CAI XIXI, HUANG MUCHEN, JIA LEE, WANG SHAOYUN: "Immunomodulatory effects of Pseudostellaria heterophylla peptide on spleen lymphocytes via a Ca 2+ /CaN/NFATc1/IFN-γ pathway", FOOD & FUNCTION, R S C PUBLICATIONS, GB, vol. 10, no. 6, 19 June 2019 (2019-06-19), GB, pages 3466 - 3476, XP055809259, ISSN: 2042-6496, DOI: 10.1039/C9FO00577C
YANG QIAN; HUANG MUCHEN; CAI XIXI; JIA LEE; WANG SHAOYUN: "Investigation on activation in RAW264.7 macrophage cells and protection in cyclophosphamide-treated mice of Pseudostellaria heterophylla protein hydrolysate", FOOD AND CHEMICAL TOXICOLOGY, PERGAMON, GB, vol. 134, 10 September 2019 (2019-09-10), GB, XP085885961, ISSN: 0278-6915, DOI: 10.1016/j.fct.2019.110816
YANG QIAN; CAI XIXI; HUANG MUCHEN; WANG SHAOYUN: "A specific peptide with immunomodulatory activity from Pseudostellaria heterophylla and the action mechanism", JOURNAL OF FUNCTIONAL FOODS, ELSEVIER BV, NL, vol. 68, 15 March 2020 (2020-03-15), NL, XP086145334, ISSN: 1756-4646, DOI: 10.1016/j.jff.2020.103887
YANG QIAN; HUANG MUCHEN; CAI XIXI; JIA LEE; WANG SHAOYUN: "Investigation on activation in RAW264.7 macrophage cells and protection in cyclophosphamide-treated mice of Pseudostellaria heterophylla protein hydrolysate", FOOD AND CHEMICAL TOXICOLOGY, PERGAMON, GB, vol. 134, 10 September 2019 (2019-09-10), GB, XP085885961, ISSN: 0278-6915, DOI: 10.1016/j.fct.2019.110816
YANG QIAN; CAI XIXI; HUANG MUCHEN; WANG SHAOYUN: "A specific peptide with immunomodulatory activity from Pseudostellaria heterophylla and the action mechanism", JOURNAL OF FUNCTIONAL FOODS, ELSEVIER BV, NL, vol. 68, 15 March 2020 (2020-03-15), NL, XP086145334, ISSN: 1756-4646, DOI: 10.1016/j.jff.2020.103887
Attorney, Agent or Firm:
FUZHOU YUANCHUANG PATENT AND TRADEMARK AGENT LTD. (CN)
Download PDF: