Title:
PREPARATION METHOD OF LIPID BODIES, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/107728
Kind Code:
A1
Abstract:
A preparation method of lipid bodies, and use thereof. Particularly, provided is a method of preparing lipid bodies consisting of neutral lipids and a phospholipid monolayer, comprising the following steps: a1) placing phospholipids and neutral lipids in a buffer solution, forming a vortex to react the same, then performing centrifugation, and collecting an upper layer having a liquid phase; a2) performing purification of the liquid phased upper layer for twice or more, wherein each purification is performed by mixing the liquid-phased upper layer with the buffer solution, then layering the mixture, and collecting an upper layer having a liquid phase; and a3) mixing the liquid-phased upper layer obtained in step a2) with the buffer solution, layering the mixture, and collecting a lower layer having a liquid phase and containing lipid bodies therein. For the lipid bodies prepared with the method, one or more intrinsic proteins and/or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins; both play an important role in preparing drugs and/or drug carriers.
Inventors:
LIU PINGSHENG (CN)
WANG YANG (CN)
ZHOU XIAOMING (CN)
MA XUEJING (CN)
ZHOU CHANG (CN)
WANG YANG (CN)
ZHOU XIAOMING (CN)
MA XUEJING (CN)
ZHOU CHANG (CN)
Application Number:
PCT/CN2016/106732
Publication Date:
June 29, 2017
Filing Date:
November 22, 2016
Export Citation:
Assignee:
INST OF BIOPHYSICS CHINESE ACAD OF SCIENCES (CN)
International Classes:
A61K9/127; A61K47/14; A61K47/24; A61K47/42; C07K14/47
Domestic Patent References:
| WO2011127456A2 | 2011-10-13 |
Foreign References:
| CN105483076A | 2016-04-13 | |||
| CN1717223A | 2006-01-04 | |||
| CN1870894A | 2006-11-29 |
Other References:
WANG Y. ET AL.: "Construction of Nanodroplet/Adiposome and Artificial Lipid Droplets", A CS NANO, 24 February 2016 (2016-02-24), pages 3312 - 3322, XP 055396748
LIU P.S. ET AL.: "Resconstitution of Adiposome and Artificial Lipid Droplets", THE FASEB JOURNAL, vol. 29, no. 1, 30 April 2015 (2015-04-30), XP009511500
KRAHMER N. ET AL.: "Phosphatidylcholine Synthesis for Lipid Droplet Expansion is Mediated by Localized Activation of CTP: Phosphocholine Cytidylyltransferase", CELL METABOLISM, 26 July 2011 (2011-07-26), pages 504 - 515, XP 028308485
WANG, XIAOMEI ET AL.: "Research Overviews Of Multivesicular Liposome", CHINESE JOURNAL OF NEW DRUGS, vol. 15, no. 15, 2006, pages 1243 - 1246, XP 009511371, [retrieved on 20061231]
See also references of EP 3395329A4
LIU P.S. ET AL.: "Resconstitution of Adiposome and Artificial Lipid Droplets", THE FASEB JOURNAL, vol. 29, no. 1, 30 April 2015 (2015-04-30), XP009511500
KRAHMER N. ET AL.: "Phosphatidylcholine Synthesis for Lipid Droplet Expansion is Mediated by Localized Activation of CTP: Phosphocholine Cytidylyltransferase", CELL METABOLISM, 26 July 2011 (2011-07-26), pages 504 - 515, XP 028308485
WANG, XIAOMEI ET AL.: "Research Overviews Of Multivesicular Liposome", CHINESE JOURNAL OF NEW DRUGS, vol. 15, no. 15, 2006, pages 1243 - 1246, XP 009511371, [retrieved on 20061231]
See also references of EP 3395329A4
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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