Title:
PREPARATION METHOD FOR MAGNETIC MICROSPHERES FOR NUCLEIC ACID EXTRACTION, PREPARED PRODUCT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/135420
Kind Code:
A1
Abstract:
Disclosed is a method for preparing silicon hydroxyl magnetic microspheres, the method comprising: providing magnetic particles; carrying out a hydrophilic layer modification on the magnetic particles; and carrying out a functionalization treatment on the magnetic particles modified by a hydrophilic layer, or, carrying out a silicon hydroxyl modification on the magnetic particles modified by the hydrophilic layer and carrying out a post-treatment with a post-treatment reagent after the silicon hydroxyl modification. The functionalization treatment comprises: dispersing the modified magnetic particles in an anhydrous lower alcohol; and adding a silicon hydroxyl-functionalized monomer for reaction, wherein water is not added and no aqueous reagent other than a pH-adjusting agent is used during the step of functionalization treatment. The post-treatment reagent is selected from at least one of an organic strong acid, an inorganic strong acid, or a mixture of an inorganic strong acid and a complexing agent. The present invention further relates to the prepared silicon hydroxyl magnetic microspheres and the use thereof in nucleic acid extraction. The present invention improves the extraction effect of nucleic acid, especially RNA, and the inter-batch stability of detection.
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Inventors:
SUN JUNZHI (CN)
LIU FENG (CN)
WANG YI (CN)
LI YING (CN)
YANG JIAO (CN)
HE TAO (CN)
LONG TENGXIANG (CN)
LIU FENG (CN)
WANG YI (CN)
LI YING (CN)
YANG JIAO (CN)
HE TAO (CN)
LONG TENGXIANG (CN)
Application Number:
PCT/CN2021/140204
Publication Date:
June 30, 2022
Filing Date:
December 21, 2021
Export Citation:
Assignee:
SICHUAN ANKERUI NEW MAT CO LTD (CN)
International Classes:
C12N15/10; H01F41/02; H01F1/00; H01F1/42
Foreign References:
| CN112563016A | 2021-03-26 | |||
| CN106237947A | 2016-12-21 | |||
| CN101085799A | 2007-12-12 | |||
| CN102115739A | 2011-07-06 |
Attorney, Agent or Firm:
YUHONG INTELLECTUAL PROPERTY LAW FIRM (CN)
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