Title:
PREPARATION METHOD FOR NUCLEIC ACID SAMPLE DERIVED FROM SKIN STRATUM CORNEUM
Document Type and Number:
WIPO Patent Application WO/2022/172991
Kind Code:
A1
Abstract:
This preparation method for a nucleic acid sample derived from the skin stratum corneum comprises the following: extracting nucleic acids from the stratum corneum sampled from the surface of the skin and preparing a water-soluble alcohol aqueous solution containing the nucleic acids; bringing the nucleic acids in the water-soluble alcohol aqueous solution into contact with a solid-phase material and isolating the nucleic acids; and amplifying, or reverse transcribing and amplifying, the isolated nucleic acids in the presence of a single-stranded nucleic acid binding protein to prepare a nucleic acid sample.
Inventors:
INOUE TAKAYOSHI (JP)
YANO MICHIKO (JP)
YANO MICHIKO (JP)
Application Number:
PCT/JP2022/005355
Publication Date:
August 18, 2022
Filing Date:
February 10, 2022
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/10; C12Q1/6806; C12Q1/6869
Domestic Patent References:
| WO2018008319A1 | 2018-01-11 | |||
| WO2016052619A1 | 2016-04-07 |
Foreign References:
| JP2016019531A | 2016-02-04 | |||
| JP2007531529A | 2007-11-08 | |||
| JP2007117084A | 2007-05-17 |
Other References:
NICHOLAS R BENSON, PAPENFUSS JASON, WONG RITA, MOTAAL AMRO, TRAN VYNGA, PANKO JACQUELINE, KRUEGER GERALD G: "An Analysis of Select Pathogenic Messages in Lesional and Non-Lesional Psoriatic Skin Using Non-Invasive Tape Harvesting", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 126, no. 10, 1 October 2006 (2006-10-01), pages 2234 - 2241, XP055034081, ISSN: 0022202X, DOI: 10.1038/sj.jid.5700412
CHANDLER DARRELL P; WAGNON CHRISTINA A; BOLTON HARVEY JR: "Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 64, no. 2, 1 February 1998 (1998-02-01), US , pages 669 - 677, XP009141630, ISSN: 0099-2240, DOI: 10.1128/AEM.64.2.669-677.1998
J. DERMATOL. SCI, vol. 101, no. 1, 2021, pages 14 - 21
APPL. ENVIRON. MICROBIOL., vol. 62, no. 3, 1996, pages 1102 - 1106
APPL. ENVIRON. MICROBIOL., vol. 64, no. 2, 1998, pages 669 - 677
BIOTECHNIQUES, vol. 31, no. 1, 2001, pages 81 - 86
PLOS ONE, vol. 5, no. 6, 2010, pages e11024
SCIENCE, vol. 227, 1985, pages 1435 - 41
CELL, vol. 155, 2013, pages 285 - 295
CANCER MANAG. RES., vol. 10, 2018, pages 1143 - 1154
CAROLINE MEYER OLESEN ET AL., SCIENTIFIC REPORTS, vol. 9, 2019, pages 12217
CHANDLER DARRELL P; WAGNON CHRISTINA A; BOLTON HARVEY JR: "Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 64, no. 2, 1 February 1998 (1998-02-01), US , pages 669 - 677, XP009141630, ISSN: 0099-2240, DOI: 10.1128/AEM.64.2.669-677.1998
J. DERMATOL. SCI, vol. 101, no. 1, 2021, pages 14 - 21
APPL. ENVIRON. MICROBIOL., vol. 62, no. 3, 1996, pages 1102 - 1106
APPL. ENVIRON. MICROBIOL., vol. 64, no. 2, 1998, pages 669 - 677
BIOTECHNIQUES, vol. 31, no. 1, 2001, pages 81 - 86
PLOS ONE, vol. 5, no. 6, 2010, pages e11024
SCIENCE, vol. 227, 1985, pages 1435 - 41
CELL, vol. 155, 2013, pages 285 - 295
CANCER MANAG. RES., vol. 10, 2018, pages 1143 - 1154
CAROLINE MEYER OLESEN ET AL., SCIENTIFIC REPORTS, vol. 9, 2019, pages 12217
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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