Title:
PREPARATION METHOD AND USE OF DEFECTIVE FILOVIRUS
Document Type and Number:
WIPO Patent Application WO/2024/001845
Kind Code:
A1
Abstract:
The present invention provides a preparation method and use of a defective filovirus. Specifically, the present invention provides a defective recombinant filovirus, a coding sequence of one or more key viral proteins in a genome of the defective recombinant filovirus being replaced with a recombinase coding sequence, and a use of the defective recombinant filovirus in the aspects of antiviral drug research and development, virology research, vaccine development, and the like. The defective recombinant filovirus of the present invention has a good biosafety feature, is easy to produce, can completely simulate related immune response of host cells caused by virus infection, and is a tool virus having extremely high practicability.
Inventors:
ZUO JIANPING (CN)
SHI ZHENGLI (CN)
TONG XIANKUN (CN)
YANG LI (CN)
YANG XINGLOU (CN)
ZHOU YU (CN)
YANG XIAOQIAN (CN)
ZHU FENGHUA (CN)
XIE SHA (CN)
LI HENG (CN)
GONG YING (CN)
SHI ZHENGLI (CN)
TONG XIANKUN (CN)
YANG LI (CN)
YANG XINGLOU (CN)
ZHOU YU (CN)
YANG XIAOQIAN (CN)
ZHU FENGHUA (CN)
XIE SHA (CN)
LI HENG (CN)
GONG YING (CN)
Application Number:
PCT/CN2023/101148
Publication Date:
January 04, 2024
Filing Date:
June 19, 2023
Export Citation:
Assignee:
SHANGHAI INST MATERIA MEDICA CAS (CN)
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCIENCE (CN)
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCIENCE (CN)
International Classes:
C12N7/01; A61K39/12; C12N5/10; C12N15/11; C12R1/93
Foreign References:
| CN112592900A | 2021-04-02 | |||
| CN101918565A | 2010-12-15 | |||
| CN102051379A | 2011-05-11 |
Other References:
TONG XIAN-KUN, LI HENG, YANG LI, XIE SHI-ZHE, XIE SHA, GONG YING, PENG CHENG, GAO XIAO-XIAO, SHI ZHENG-LI, YANG XING-LOU, ZUO JIAN: "Multiplication of defective Ebola virus in a complementary permissive cell line", ANTIVIRAL RESEARCH, ELSEVIER BV, NL, vol. 209, 1 January 2023 (2023-01-01), NL , pages 105491, XP093121961, ISSN: 0166-3542, DOI: 10.1016/j.antiviral.2022.105491
ANDRAS NAGY: "Cre recombinase: The universal reagent for genome tailoring", GENESIS, WILEY-LISS, HOBOKEN, USA, vol. 26, no. 2, 23 February 2000 (2000-02-23), Hoboken, USA, pages 99 - 109, XP072302741, ISSN: 1526-954X, DOI: 10.1002/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B
ANDRAS NAGY: "Cre recombinase: The universal reagent for genome tailoring", GENESIS, WILEY-LISS, HOBOKEN, USA, vol. 26, no. 2, 23 February 2000 (2000-02-23), Hoboken, USA, pages 99 - 109, XP072302741, ISSN: 1526-954X, DOI: 10.1002/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
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