Title:
PREPARATION AND SELECTION OF DONOR CELLS FOR NUCLEAR TRANSPLANTATION
Document Type and Number:
WIPO Patent Application WO2001026454
Kind Code:
A9
Abstract:
The invention relates to a method of synchronizing a population of somatic cells in G1 for purposes of preparing the cells for nuclear transfer or nuclear transplantation by using mechanical shake-off and selection of mitotic doublet cells. This method may further comprise cooling of the cells or other means of synchronizing the cells in G1 phase for longer periods of time. The invention also relates to the use of a synchronized population of rapidly, dividing somatic cells obtained by these methods as a source of donor nuclei or chromatin for use in nuclear transfer or nuclear transplantation.
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Inventors:
ROBL JAMES M (US)
POOTHAPPILLAI KASINATHAN (US)
KNOTT JASON G (US)
JERRY JOSEPH D (US)
POOTHAPPILLAI KASINATHAN (US)
KNOTT JASON G (US)
JERRY JOSEPH D (US)
Application Number:
PCT/US2000/028287
Publication Date:
August 01, 2002
Filing Date:
October 13, 2000
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
ROBL JAMES M (US)
POOTHAPPILLAI KASINATHAN (US)
KNOTT JASON G (US)
JERRY JOSEPH D (US)
ROBL JAMES M (US)
POOTHAPPILLAI KASINATHAN (US)
KNOTT JASON G (US)
JERRY JOSEPH D (US)
International Classes:
A01K67/00; C12N15/85; C12N15/873; A01K67/027; (IPC1-7): A01K67/00; C12N5/00; C12N5/02; C12N15/00
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