Title:
PREPARATION AND TRANSFORMATION METHODS FOR BACILLUS SUBTILIS COMPETENT CELL
Document Type and Number:
WIPO Patent Application WO/2021/062886
Kind Code:
A1
Abstract:
A cell wall weakening agent for preparing a bacillus subtilis competent cell, comprising the following components: glycine, serine, and dithiothreitol. The provided cell wall weakening agent can improve the transformation efficiency of bacillus subtilis. Also provided is a transformation method for a bacillus subtilis competent cell by electroporation.
Inventors:
ZHOU HUI (CN)
Application Number:
PCT/CN2019/111571
Publication Date:
April 08, 2021
Filing Date:
October 17, 2019
Export Citation:
Assignee:
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N1/20; C12N15/75; C12R1/125
Domestic Patent References:
| WO2017044476A1 | 2017-03-16 |
Foreign References:
| CN109266676A | 2019-01-25 | |||
| CN102212546A | 2011-10-12 | |||
| CN102649967A | 2012-08-29 |
Other References:
LI RUIFANG, XUE WENWEN, HUANG LIANG, XIONG QIANCHENG, WANG BIN: "Competent Preparation and Plasmid Transformation of Bacillus subtilis", BIOTECHNOLOGY BULLETIN, no. 5, 1 January 2011 (2011-01-01), pages 227 - 230, XP055796788, DOI: 10.13560/j.cnki.biotech.bull.1985.2011.05.035
KELLEY DANIELLE S., LENNON CHRISTOPHER W., BELFORT MARLENE, NOVIKOVA OLGA: "Mycobacteriophages as Incubators for Intein Dissemination and Evolution", MBIO, vol. 7, no. 5, 2 November 2016 (2016-11-02), XP055796776, DOI: 10.1128/mBio.01537-16
KELLEY DANIELLE S., LENNON CHRISTOPHER W., BELFORT MARLENE, NOVIKOVA OLGA: "Mycobacteriophages as Incubators for Intein Dissemination and Evolution", MBIO, vol. 7, no. 5, 2 November 2016 (2016-11-02), XP055796776, DOI: 10.1128/mBio.01537-16
Attorney, Agent or Firm:
SCIHEAD IP LAW FIRM (CN)
Download PDF: