Title:
PREPARATION AND USE OF THERMOPHORIC Β-GLUCOSIDASE
Document Type and Number:
WIPO Patent Application WO/2021/217902
Kind Code:
A1
Abstract:
Preparation and use of a thermophoric β-glucosidase, belonging to the technical field of genetic engineering and fermentation engineering. By constructing a recombinant fungus, β-glucosidase TpBgl3A derived from thermophilic fungus Talaromyces piceae is heterologously expressed, and the recombinant fungus can produce the enzyme up to 2324 U/mL in a 3.6L fermenter. The beta-glucosidase TpBgl3A produced can prepare an gentiooligsaccharide with a high conversion rate using glucose as a substrate at a relatively low enzyme addition amount, thereby significantly reducing production costs; and in a reaction system using glucose and cellobiose as substrates, has a conversion rate of the gentiooligsaccharide up to 26.2%, having the potential of industrial production of the gentiooligsaccharide.
Inventors:
WU JING (CN)
XIA WEI (CN)
XU XINGHAO (CN)
HUANG YAN (CN)
XIA WEI (CN)
XU XINGHAO (CN)
HUANG YAN (CN)
Application Number:
PCT/CN2020/102671
Publication Date:
November 04, 2021
Filing Date:
July 17, 2020
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/42; C12N1/19; C12N15/56; C12N15/81; C12P19/00; C12P19/12; C12P19/14
Foreign References:
| CN101492661A | 2009-07-29 | |||
| CN107099565A | 2017-08-29 | |||
| CN105238827A | 2016-01-13 |
Other References:
DATABASE PROTEIN 2017-11-07, ANONYMOUS : "beta-glucosidase [Talaromyces piceae]", XP055862850, retrieved from NCBI Database accession no. ATQ35964
LIU LINGLING, ZHU SONG ZHU TING ZHANG MIN WU JING CHEN JIAN: "Production of Gentiooligosaccharide by Recombinant β-Glucosidase", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 49, no. 5, 4 May 2009 (2009-05-04), CN , pages 598 - 603, XP055863055, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.2009.05.006
HE RONGLIN, BAI XUE, CAI PENGLIN, SUN CHENG, ZHANG DONGYUAN, CHEN SHULIN: "Genome sequence of Talaromyces piceus 9-3 provides insights into lignocellulose degradation", 3 BIOTECH, SPRINGEROPEN, DE, vol. 7, no. 6, 1 December 2017 (2017-12-01), DE , XP055862851, ISSN: 2190-572X, DOI: 10.1007/s13205-017-1001-5
HE RONGLIN; CAI PENGLI; WU GAIHONG; ZHANG CAN; ZHANG DONGYUAN; CHEN SHULIN: "Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis ofTalaromyces piceus", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER NETHERLANDS, DORDRECHT, vol. 31, no. 11, 2 September 2015 (2015-09-02), Dordrecht, pages 1811 - 1819, XP035538369, ISSN: 0959-3993, DOI: 10.1007/s11274-015-1934-y
LIU LINGLING, ZHU SONG ZHU TING ZHANG MIN WU JING CHEN JIAN: "Production of Gentiooligosaccharide by Recombinant β-Glucosidase", ACTA MIEROBIOLOGICA SINICA, KEXUE CHUBANSHE, BEIJING, CN, vol. 49, no. 5, 4 May 2009 (2009-05-04), CN , pages 598 - 603, XP055863055, ISSN: 0001-6209, DOI: 10.13343/j.cnki.wsxb.2009.05.006
HE RONGLIN, BAI XUE, CAI PENGLIN, SUN CHENG, ZHANG DONGYUAN, CHEN SHULIN: "Genome sequence of Talaromyces piceus 9-3 provides insights into lignocellulose degradation", 3 BIOTECH, SPRINGEROPEN, DE, vol. 7, no. 6, 1 December 2017 (2017-12-01), DE , XP055862851, ISSN: 2190-572X, DOI: 10.1007/s13205-017-1001-5
HE RONGLIN; CAI PENGLI; WU GAIHONG; ZHANG CAN; ZHANG DONGYUAN; CHEN SHULIN: "Mutagenesis and evaluation of cellulase properties and cellulose hydrolysis ofTalaromyces piceus", WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER NETHERLANDS, DORDRECHT, vol. 31, no. 11, 2 September 2015 (2015-09-02), Dordrecht, pages 1811 - 1819, XP035538369, ISSN: 0959-3993, DOI: 10.1007/s11274-015-1934-y
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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