Title:
PRIMER FOR DETECTING BOVINE DNA AND DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2024/017393
Kind Code:
A1
Abstract:
A primer pair for detecting bovine genomic DNA, a detection reagent or kit containing the primer pair, and a method for detecting bovine genomic DNA by using the primer pair, the primer pair specifically binding to the sequence represented by SEQ ID NO: 1. A PCR detection method using the primer pair has quick and convenient operation and high sensitivity, and can distinguish interfering DNA such as pig, CHO, Vero, human, NS0, MDCK, E.coli, Pichia pastoris and Sf9.
Inventors:
SHAO ANLIANG (CN)
WU WANXIN (CN)
CHEN LIANG (CN)
ZONG WEIYING (CN)
LI KANGKAI (CN)
WU XIAOSHUANG (CN)
WEI LINA (CN)
DUAN XIAOJIE (CN)
FAN XINGLIANG (CN)
CHEN LIYUAN (CN)
ZHANG XIAO (CN)
XU LIMING (CN)
LI JINGLI (CN)
WU WANXIN (CN)
CHEN LIANG (CN)
ZONG WEIYING (CN)
LI KANGKAI (CN)
WU XIAOSHUANG (CN)
WEI LINA (CN)
DUAN XIAOJIE (CN)
FAN XINGLIANG (CN)
CHEN LIYUAN (CN)
ZHANG XIAO (CN)
XU LIMING (CN)
LI JINGLI (CN)
Application Number:
PCT/CN2023/108963
Publication Date:
January 25, 2024
Filing Date:
July 24, 2023
Export Citation:
Assignee:
NAT INSTITUTES FOR FOOD AND DRAG CONTROL (CN)
HUZHOU SHENKE BIOTECHNOLOGY CO LTD (CN)
HUZHOU SHENKE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/6888; C12N15/11; C12Q1/686
Foreign References:
| CN116064826A | 2023-05-05 | |||
| CN103937881A | 2014-07-23 | |||
| CN101265500A | 2008-09-17 | |||
| CN101270357A | 2008-09-24 |
Other References:
MENDOZA-ROMERO LUIS, VERKAAR EDWARD L.C., SAVELKOUL PAUL H., CATSBURG ARNOLD, AARTS HENK J.M., BUNTJER JAAP B., LENSTRA JOHANNES A: "Real-Time PCR Detection of Ruminant DNA", JOURNAL OF FOOD PROTECTION, INTERNATIONAL ASSOCIATION FOR FOOD PROTECTION, US, vol. 67, no. 3, 1 March 2004 (2004-03-01), US , pages 550 - 554, XP093130572, ISSN: 0362-028X, DOI: 10.4315/0362-028X-67.3.550
CONCANNON, S.P. ET AL.: "A Quantitative PCR Method to Quantify Ruminant DNA in Porcine Crude Heparin", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 399, 7 November 2010 (2010-11-07), XP019869669, DOI: 10.1007/s00216-010-4362-8
WALKER JERILYN A, HUGHES DAVID A, ANDERS BRIDGET A, SHEWALE JAIPRAKASH, SINHA SUDHIR K, BATZER MARK A: "Quantitative intra-short interspersed element PCR for species-specific DNA identification", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 316, no. 2, 1 May 2003 (2003-05-01), Amsterdam, NL , pages 259 - 269, XP093130573, ISSN: 0003-2697, DOI: 10.1016/S0003-2697(03)00095-2
DATABASE Nucleotide 23 October 2008 (2008-10-23), ANONYMOUS : "B.taurus DNA for SINE sequence Bov-1D", XP093130577, Database accession no. X64126.1
CONCANNON, S.P. ET AL.: "A Quantitative PCR Method to Quantify Ruminant DNA in Porcine Crude Heparin", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 399, 7 November 2010 (2010-11-07), XP019869669, DOI: 10.1007/s00216-010-4362-8
WALKER JERILYN A, HUGHES DAVID A, ANDERS BRIDGET A, SHEWALE JAIPRAKASH, SINHA SUDHIR K, BATZER MARK A: "Quantitative intra-short interspersed element PCR for species-specific DNA identification", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 316, no. 2, 1 May 2003 (2003-05-01), Amsterdam, NL , pages 259 - 269, XP093130573, ISSN: 0003-2697, DOI: 10.1016/S0003-2697(03)00095-2
DATABASE Nucleotide 23 October 2008 (2008-10-23), ANONYMOUS : "B.taurus DNA for SINE sequence Bov-1D", XP093130577, Database accession no. X64126.1
Attorney, Agent or Firm:
XU & PARTNERS, LLC. (CN)
Download PDF: