Title:
PRIMER GROUP AND METHOD FOR DETECTING SINGLE-BASE MUTATIONS
Document Type and Number:
WIPO Patent Application WO/2022/222937
Kind Code:
A1
Abstract:
A primer group and method for detecting single-base mutations. The primer group comprises the following primers: an identification primer, which is composed of, from the 5' end to the 3' end, (a) a nucleotide sequence which complements a segment of continuous nucleotides in a nucleic acid sequence to be detected, wherein the 5' end of the continuous nucleotides starts at a first nucleotide downstream of an expected mutation site; and (b) a nucleotide which complements a non-mutated nucleotide or an expected mutated nucleotide at the expected single-base mutation site of the nucleic acid sequence. The primer group also comprises an amplification primer. The amplification primer is capable of using the identification primer to amplify an amplification product which is obtained by amplifying the nucleic acid sequence. The identification primer is 1 to 19 nucleotides less than the amplification primer.
Inventors:
HU FEICHI (CN)
WANG QI (CN)
WU ZHENGXIAN (CN)
SARAF NILESHI (CN)
WANG QI (CN)
WU ZHENGXIAN (CN)
SARAF NILESHI (CN)
Application Number:
PCT/CN2022/087791
Publication Date:
October 27, 2022
Filing Date:
April 20, 2022
Export Citation:
Assignee:
NANJING GENSCRIPT BIOTECH CO LTD (CN)
International Classes:
C12Q1/68; C12N15/11; C12Q1/6858; C12Q1/6886
Domestic Patent References:
| WO2018095401A1 | 2018-05-31 | |||
| WO2019162487A1 | 2019-08-29 |
Foreign References:
| CN109136345A | 2019-01-04 | |||
| US20030124544A1 | 2003-07-03 | |||
| US20100317019A1 | 2010-12-16 | |||
| CN102399872A | 2012-04-04 |
Other References:
"Experimental Techniques in Biochemistry and Molecular Biology", 30 June 2000, ZHEJIANG UNIVERSITY PRESS, CN, ISBN: 7-308-02280-3, article LI, CHAOLONG: "Analysis of single base mutations in genes by allele-specific amplification", pages: 222 - 224, XP009541787
Attorney, Agent or Firm:
ZHONG LUN LAW FIRM (CN)
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