Title:
PRIMER FOR SPECIFIC DETECTION OF HUMAN SOURCE GENOMIC DNA AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/243978
Kind Code:
A1
Abstract:
The present invention discloses a method for detecting human source DNA by specifically detecting human SRGAP2 gene, primer for specific detection of human SRGAP2 gene and fluorescent probe, and a TaqMan qPCR method that can be used to detect human source DNA in animal organs, tissues, cells, body fluids and blood, this method is suitable for detecting human DNA in any animal source sample, including but not limited to the biodistribution, metabolism and residence of cell and gene therapy products in the treated animal.
Inventors:
GAO GE (CN)
ZHOU ANYU (CN)
ZHOU ANYU (CN)
Application Number:
PCT/CN2019/090571
Publication Date:
December 10, 2020
Filing Date:
June 10, 2019
Export Citation:
Assignee:
IXCELL BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/11; C12Q1/6851
Domestic Patent References:
| WO2012061510A2 | 2012-05-10 |
Foreign References:
| CN110218770A | 2019-09-10 |
Other References:
HO NGUYEN THI; KIM POK-SON; KUTZNER ARNE; HEESE KLAUS: "Cognitive Functions: Human vs. Animal - 4:1 Advantage |-FAM72-SRGAP2-|", JOURNAL OF MOLECULAR NEUROSCIENCE, vol. 61, no. 4, 2 March 2017 (2017-03-02), pages 603` - 606, XP036192923, ISSN: 0895-8696, DOI: 10.1007/s12031-017-0901-5
YUH-CHIN T HUANG , MICHAEL SCHMITT , ZHONGHUI YANG , LORETTA G QUE , JUDITH C STEWART , MARK W FRAMPTON , ROBERT B DELVIN: "Gene Expression Profile in Circulating Mononuclear Cells after Exposure to Ultrafine Carbon Particles.", INHALATION TOXICOLOGY, vol. 22, no. 10, 31 August 2010 (2010-08-31), pages 835 - 846, DOI: 10.3109/08958378.2010.486419
HUANG YUH-CHIN T; SCHMITT MICHAEL; YANG ZHONGHUI; QUE LORETTA G; STEWART JUDITH C; FRAMPTON MARK W; DEVLIN ROBERT B: "Gene Expression Profile in Circulating Mononuclear Cells after Exposure to Ultrafine Carbon cParticles.", INHALATION TOXICOLOGY, vol. 22, no. 10, 31 August 2010 (2010-08-31), pages 835 - 846, XP009524888, ISSN: 0895-8378, DOI: :10.3109/08958378.2010.486419
DATABASE GenBank 17 June 2018 (2018-06-17), ROCKS, O ET AL.: "Mus musculus SRGAP2 gene for SRGAP2", XP055764647, retrieved from NCBI
YUH-CHIN T HUANG , MICHAEL SCHMITT , ZHONGHUI YANG , LORETTA G QUE , JUDITH C STEWART , MARK W FRAMPTON , ROBERT B DELVIN: "Gene Expression Profile in Circulating Mononuclear Cells after Exposure to Ultrafine Carbon Particles.", INHALATION TOXICOLOGY, vol. 22, no. 10, 31 August 2010 (2010-08-31), pages 835 - 846, DOI: 10.3109/08958378.2010.486419
HUANG YUH-CHIN T; SCHMITT MICHAEL; YANG ZHONGHUI; QUE LORETTA G; STEWART JUDITH C; FRAMPTON MARK W; DEVLIN ROBERT B: "Gene Expression Profile in Circulating Mononuclear Cells after Exposure to Ultrafine Carbon cParticles.", INHALATION TOXICOLOGY, vol. 22, no. 10, 31 August 2010 (2010-08-31), pages 835 - 846, XP009524888, ISSN: 0895-8378, DOI: :10.3109/08958378.2010.486419
DATABASE GenBank 17 June 2018 (2018-06-17), ROCKS, O ET AL.: "Mus musculus SRGAP2 gene for SRGAP2", XP055764647, retrieved from NCBI
Attorney, Agent or Firm:
HANRAY LAW FIRM (CN)
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