Title:
PRIMING OF PLURIPOTENT STEM CELLS FOR NEURAL DIFFERENTIATION
Document Type and Number:
WIPO Patent Application WO/2012/135621
Kind Code:
A3
Abstract:
Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGFβ. As an advantage, the neural cells may be provided with improved consistency and purity.
Inventors:
MEYER NATHAN (US)
STANKEWICZ CASEY (US)
RAJESH DEEPIKA (US)
GEROGE MATTHEW (US)
STANKEWICZ CASEY (US)
RAJESH DEEPIKA (US)
GEROGE MATTHEW (US)
Application Number:
PCT/US2012/031457
Publication Date:
November 22, 2012
Filing Date:
March 30, 2012
Export Citation:
Assignee:
CELLULAR DYNAMICS INT INC (US)
MEYER NATHAN (US)
STANKEWICZ CASEY (US)
RAJESH DEEPIKA (US)
GEROGE MATTHEW (US)
MEYER NATHAN (US)
STANKEWICZ CASEY (US)
RAJESH DEEPIKA (US)
GEROGE MATTHEW (US)
International Classes:
C12N5/079; A61K35/12; A61K35/30; C12N5/02
Domestic Patent References:
| WO2010063848A1 | 2010-06-10 |
Other References:
SMUKLER ET AL.: "Embryonic stem cells assume a primitive neural stem cell fate in the absence of extrinsic influences", THE JOURNAL OF CELL BIOLOGY, vol. 172, no. 1, 3 January 2006 (2006-01-03), pages 79 - 90, XP008147476
STAVRIDIS ET AL.: "A discrete period of FGF-induced Erkl/2 signalling is required for vertebrate neural specification", DEVELOPMENT, vol. 134, 2007, pages 2889 - 2894, XP002530550
TROPEPE ET AL.: "Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism", NEURON, vol. 30, April 2001 (2001-04-01), pages 65 - 78, XP002202327
COHEN ET AL.: "The role of FGF-signaling in early neural specification of human embryonic stem cells", DEVELOPMENTAL BIOLOGY, vol. 340, 10 February 2010 (2010-02-10), pages 450 - 458, XP055098558
GREBER ET AL.: "FGF signalling inhibits neural induction in human embryonic stem cells", THE EMBO JOURNAL, vol. 30, 15 November 2011 (2011-11-15), pages 4874 - 4884, XP055098560
LI ET AL.: "Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors", PNAS, vol. 108, no. 20, 17 May 2011 (2011-05-17), pages 8299 - 8304, XP055098564
STAVRIDIS ET AL.: "A discrete period of FGF-induced Erkl/2 signalling is required for vertebrate neural specification", DEVELOPMENT, vol. 134, 2007, pages 2889 - 2894, XP002530550
TROPEPE ET AL.: "Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism", NEURON, vol. 30, April 2001 (2001-04-01), pages 65 - 78, XP002202327
COHEN ET AL.: "The role of FGF-signaling in early neural specification of human embryonic stem cells", DEVELOPMENTAL BIOLOGY, vol. 340, 10 February 2010 (2010-02-10), pages 450 - 458, XP055098558
GREBER ET AL.: "FGF signalling inhibits neural induction in human embryonic stem cells", THE EMBO JOURNAL, vol. 30, 15 November 2011 (2011-11-15), pages 4874 - 4884, XP055098560
LI ET AL.: "Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors", PNAS, vol. 108, no. 20, 17 May 2011 (2011-05-17), pages 8299 - 8304, XP055098564
Attorney, Agent or Firm:
PARKER, David, L. (98 San Jacinto Boulevard Suite 110, Austin TX, US)
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