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Title:
PROBE MELTING CURVE ANALYSIS -BASED MULTIPLEX NUCLEIC ACID DETECTION METHOD AND KIT
Document Type and Number:
WIPO Patent Application WO/2022/179419
Kind Code:
A1
Abstract:
The present invention relates to a probe melting curve analysis-based multiplex nucleic acid detection method. The method comprises the following steps: step A: designing an asymmetric PCR system; step B: PCR amplification; and step C: melting curve analysis. The present invention solves the significant problem that traditional Taqman technology cannot perform melting curve analysis after amplification so as to further judge whether a probe region sequence is completely consistent with a target sequence. The present invention can perform melting curve analysis after PCR amplification, can improve the detection multiplicity of fluorescence PCR, and can also perform genotyping and quantification at the same time.

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Inventors:
LEI XIANGDONG (CN)
Application Number:
PCT/CN2022/076520
Publication Date:
September 01, 2022
Filing Date:
February 16, 2022
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Assignee:
SHANGHAI ACEBIOX BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/68
Foreign References:
CN107849603A2018-03-27
JP2003334078A2003-11-25
CN103228796A2013-07-31
US20030059807A12003-03-27
JP2004248678A2004-09-09
Attorney, Agent or Firm:
BEIJING TROHENG INTELLECTUAL PROPERTY LAW FIRM (CN)
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