Title:
PROCESS FOR DNA LABELLING
Document Type and Number:
WIPO Patent Application WO/1987/007611
Kind Code:
A1
Abstract:
A process for labelling DNA fragments (oligonucleotides) using fluorescent dyes. The labelling is carried out by converting the DNA fragment into its 5'-[HS-(Y)z]-derivative, and the latter is reacted with a derivative of a fluorescent dye, which together with the 5'-[HS-(Y)z]-derivative forms a DNA fragment of the formula (I) fluorophore-X-S-(Y)z-CH$(2,6)$ -oligonucleotide, wherein X and Y independently are a group comprising one or more carbon atoms and/or hetero atoms, z = 0 or 1, and fluorophore is the residue of a fluorescent dye. In a preferred embodiment the DNA fragment is converted into its 5'-(S-triphenylmethyl-3-mercaptopropyl phospho)-derivative and the 5'-(3-mercaptopropyl phospho)-derivative obtained after cleavage of the triphenylmethyl group is reacted with a (5- and/or 6)-iodoacetamino-rhodamine or -fluorescein.
Inventors:
SPROAT BRIAN (DE)
Application Number:
PCT/EP1987/000287
Publication Date:
December 17, 1987
Filing Date:
June 03, 1987
Export Citation:
Assignee:
EUROP LAB MOLEKULARBIOLOG (DE)
International Classes:
C07H21/00; (IPC1-7): C07H21/04; C12Q1/68
Foreign References:
| EP0070685A2 | 1983-01-26 | |||
| FR2556726A1 | 1985-06-21 |
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