Title:
PROCESS FOR EXTRACTING PULLULAN POLYSACCHARIDE FROM HIGH-VISCOSITY FERMENTATION BROTH
Document Type and Number:
WIPO Patent Application WO/2023/061065
Kind Code:
A1
Abstract:
The present invention provides a process for extracting pullulan polysaccharide from high-viscosity fermentation broth. The process comprises the following steps: (1) removing thalli from the fermentation broth; (2) removing protein; (3) performing macroporous adsorption resin discoloration; (4) removing ions by means of ultrafiltration; and (5) drying, crushing and packaging. The extraction process in the present invention uses chitosan as a natural polymeric biogenic flocculant, does not need the high-viscosity fermentation broth to be diluted, does not require the addition of filter aids and organic solvents for alcohol precipitation, and reduces the pressure of subsequent decolorization. Moreover, bacterial protains can be properly recycled, thereby avoiding the potential hazard of the organic solvents. The method can obtain high-purity pullulan polysaccharide, improve the product yield and quality, reduce solid waste, reduce the production cost, and achieve a safe, efficient, continuous and automated production process.
Inventors:
QIAO CHANGSHENG (CN)
CHENG GANGGANG (CN)
LU LIANG (CN)
ZHAO TINGBIN (CN)
LI ZHENHAI (CN)
CHENG GANGGANG (CN)
LU LIANG (CN)
ZHAO TINGBIN (CN)
LI ZHENHAI (CN)
Application Number:
PCT/CN2022/114968
Publication Date:
April 20, 2023
Filing Date:
August 26, 2022
Export Citation:
Assignee:
TIANJIN UNIV OF SCIENCE AND TECHNOLOGY (CN)
International Classes:
C08B37/02
Foreign References:
| CN113735991A | 2021-12-03 | |||
| CN103172757A | 2013-06-26 | |||
| CN107557399A | 2018-01-09 | |||
| US20030225263A1 | 2003-12-04 |
Attorney, Agent or Firm:
TIANJIN SHANGWEN INTELLECTUAL PROPERTY AGENCY CO. LTD (CN)
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