ARTOCARPUS LAKOOCHA ROXB

Field of the invention:

The present invention relates to a process for the extraction and separation of oxyresveratrol from the heartwood of Artocarpus lakoocha Roxb. More particularly, the present invention relates to a process for separation of oxyresveratrol molecule from the extracted solution of Artocarpus lakoocha Roxb. through membrane application. Oxyresveratrol is a pharmacologically active compound and has its demand as an anti-aging, antioxidant, cardio-protective, anti-cancer and neuroprotective properties.

Background of the invention

The plant Artocarpus lakoocha Roxb. is a locally available plant in Assam and found plentifully in unreserved forest area. The heartwood of Artocarpus lakoocha Roxb. contains varieties of pharmacologically active chemical constituents such as artocarpin, norartocarpin, norcycloartocarpin, resorcinol and oxyresveratrol. Oxyresveratrol (2, 3', 4, 5'-tetrahydroxystilbene) content of it is selected for extraction and separation because of its ever-growing demand as an anti- aging, cardio-protective, anti-cancerous properties besides having its use for treatment of tape -worm infestation. Neuroprotective effects of oxyresveratrol against neurodegradation in Alzheimer's disease has also been referred in recent advances on nutrition and prevention of Alzheimer's disease. The selection of appropriate solvent for the extraction of oxyresveratrol from the heartwood of Artocarpus lakoocha Roxb. is a challenging one because some common used solvents such as water, methanol, ethanol etc. are defined as ecofriendly. Dilute aqueous mineral acid solution is also tested for the extraction of oxyresveratrol and it is found that use of dilute aqueous mineral acid has no significant effect on extraction. Extraction in pure water is found to be quite promising for more than 80% w/w extraction of oxyresveratrol from Artocarpus lakoocha Roxb. For separation of oxyresveratrol from aqueous solution, membrane technique using indigenously prepared nanofiltration (NF) membrane was used which were characterized by Pore diameter (PMI, Model CCFP-5A), Scanning Electron Microscope (SEM) (LEO 1400VP, UK), Transmission Electron Microscope (TEM) (JEOL, Japan, JEM 2100), IR (PERKIN Elmer System 2000), XRD (JDX-11P-3A, JEOL, Japan), TGA-DTA (Perkin Elmer PC series DSC 7) analysis. The technique has the special advantage because the same extractant which comes out from the membrane cell as permeate can be reused.

The oxyresveratrol molecule is recovered from the membrane cell by backwashing the membrane cell with ethanol under a pressure limit within the NF range. The following are the related prior art references so far available in the literature for extraction and separation of oxyresveratrol from Artocarpus lakoocha Roxb. /. Journal of Medicinal Plants Research, 2010, 4(10), 947-953

Title: Antioxidant and toxicity activities of Artocarpus lakoocha Roxb. heartwood extract

In this paper the antioxidant activity of Artocarpus lakoocha heartwood extract was investigated from ethanol extraction by 2,2 ^, -azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) decolorization, 2,2-diphenyl-l -pierylbydrazyl (DPPH) radical and H ₂ 0 ₂ scavenging assay, Polyphenolic; total phenolic, fiavonoids and tannins were measured. Anti-oxidative stress was studied in AAPH-oxidized blood and glutathione (GSH), and malondialdehyde (MDA) was evaluated. Results showed that antioxidant activities were 128.30 ± 0.13, 55.86 + 0.01, and 463.49 ± 0.01 μπκΛ Trolox/g extracted from ABTS, DPPH, and H ₂ 0 ₂ scavenging methods. One gram of extract contained total phenol (325.63 ± 2.99 mg GE), fiavonoids (521.98 + 0-01 mg QE) and tannins (124.03 + 0.46 g TE), including rutin and resocinol. In the blood system, a low concentration of extract inhibited MDA progression and improved GSH, which was in contrast to a high concentration with its toxicity effect.

2. Medical Principles and Practice, 2009, 18, 223-227

Title: Quantitative Analysis of Oxyresveratrol Content in Artocarpus lakoocha and 'Puag-Haad'

In this work a thin-layer chromatography (TLC) densitometric method for the determination of oxyresveratrol content in Artocarpus lakoocha heartwood was developed. The amounts of oxyresveratrol in 3 samples of A. lakoocha heartwood collected from its natural habitat were 49.0- 182.3 mg/g, whereas those in 11 commercial samples were in the range of 23.4-69.6 mg/g. The Oxyresveratrol contents in 2 samples of traditional drug Puag-Haad were 780.1 and 837.5 mg/g.

3. Maejo International Journal of Science and Technology, 2010, 4(03), 454-461

Title: Antiglycation and antioxidant activities of Oxyresveratrol extracted from the heartwood of Artocarpus lakoocha Roxb.

In this work the heartwood of Artocarpus lakoocha, oxyresveratrol was isolated with a yield of 10%, The isolated oxyresveratrol showed strong antiglycation and antioxidant activities. The IC50 value for antiglycation was 2.0+0.03 g ml (five times higher than that of aminoguanidine), and the IC50 values for antioxidation were 0.1+0.01 mg/ml (DPPH method) and 0.43+0.03 mg/ml (TEARS method), which were nearly twice as strong as those of resveratrol. 4, J Health Res 2007, 21(4), 257-262

Title: Pharmacognostic Study of Artocarpus lakooch Heartwood In this paper pharmaeognostic study of Artocarpus lakoocha heartwood was performed on 13 samples collected from five different geographical areas of Thailand. Evaluation of the crude drug was conducted according to the World Health Organization (WHO) guidelines for herbal standardization. Microscopic examination of the powdered drug revealed the presence of parenchyma and fiber cells of the medullary ray, as well as bordered pored tracheids and vessels. The contents of foreign matter, acid-soluble ash, total ash, moisture and oxyresveratrol were determined to be 0.04, 2.06, 2.51, 9.57 and 1.44 %, respectively, whereas the ethanol-soluble extractive, water-soluble extractive and loss on drying values were found to be 7.93, 5.27 and 9.79 . In addition, a thin-layer chromatographic system for rapid detection of oxyresveratrol was described, and a method for quantitative analysis of oxyresveratrol content in the crude drug using capillary zone electrophoretic technique was developed.

5. Journal of Applied Polymer Science, 2012, 125, 3888-3898

Title: Preparation and Characterization of Polysulfone-Cyclodextrin Composite Nanofiltration Membrane: Solvent Effect

In this work -CycIodextrin membranes were prepared by the phase inversion method using four types of casting solvents such as N-methyl pyrrolidone (NMP), dimethyl sulfoxide (DMSO), dimethyl acetamide (DMAc), and dimethyl formamide (DMF) herein-after termed as ce-CD-NMP, a- CDDMSO,a-CD-DMAc, and a oc-CD-DMF, respectively. The membranes were characterized by IR, XRD, TGA-DTA, DSC, and SEM analysis and show that solvents like NMP, DMA, DMF give good uniform morphological membranes and are better than that of DMSO, Thermal decompositions of the pure polymer and composite memhranes indicate different range of thermal degradation of the memhrane. This study reveals that the casting solvents NMP, DMF, DMAC have nearly same significant effect on morphology and other properties of the membranes. This is explained in terms of demixing behavior of the polymer and the combined effect of solvent volatility and polymer-solvent interactions as estimated from Hansen solubility parameter. Solvent hydrophobicity also affects the performance of the membrane and can be determined in terms of water permeability. 6. Tropical Journal of Pharmaceutical Research, 2012, 11 (1), 69-74

Title: Anti-Aging Activity and Non-Toxic Dose of Phytooxyresveratrol from Artocarpus lakoocha Roxb.

In this work the anti-aging activity and toxicity doses of phytooxyresveratrol extracted from Artocarpus lakoocha Roxb. were studied. Artocarpus lakoocha 100 g was extracted with 2 ml of 95 % ethanol to obtain phytooxyresveratrol (POV). Total phenolic content, as well as free radical scavenging and anti-glycation activities of POV were characterized in order to assess its anti-aging properties. The models of DNA nicking and bacterial reverse mutation (Ames) were applied to the extract in order to determine its effective and toxic doses, respectively,

7. Journal of Science Technology Mahasarakham University, 2008, 27(2), 100-109

Title: Influence of Extractive Methods on Chemical Constituents and Antioxidative Capacity of Artocarpus lakoocha Heartwood

In this work, phytoalexin have been reported in a genus Artocarpus. Analytical methods for measuring resveratrol and resorcinol in Artocarpus lakaoeha heartwood extracts were adapted to isolate with several methods (distill extract, aqueous extract, methanolic extract and methanolic extract in a Soxhlet extractor), and analyzed by reversed phase HPLC using a C-18 column. Total phenolic content was determined spectrophotometrically with phenol Folin-Ciocalteauus reagent, and antioxidative capacity employing the l,l-diphenyI-2-picryIhydrazyI stable free radical (DPPH).

8. Shizhen Guoyi Guoyao, 2009, 20(1), 203-204

Title: Membrane separation technology used in purification of resveratrol

The membrane sepn. technol. used in the purifn. of resveratrol and reducing pollution were reported in this work. After liq. state fermn., resveratrol was extd. by 60% ethanol at room temp., solid/liq. ratio 1 :8, the filtrate was obtained by filtering, and dealt it with two membrane equipments, the purity of resveratrol was detected by HPLC, The purity of resveratrol reached 30.5% after the microfiltration membrane, and after the ultrafiltration memhrane the purity of resveratrol could reach 55.8%. This method can reduce the cost of prodn. without toxic and harmful solvents and it can realize clean production.

9. CN102219652-B

Title: Method for preparing water-soluble resveratrol from giant knotweed rhizome

This invention provides a method for preparing water-soluble resveratrol from giant knotweed rhizome. Specifically, giant knotweed rhizome as the raw material is subjected to a healing treatment, pulverization and fermentation, and then extracted by methylal. The extract generated then undergoes condensation, membrane separation, macroporous resin adsorption and elution. And the eluate obtained iscondensed, recrystallized and dried in vacuum, thus obtaining resveratrol. Next, by the preparation method of chitosan nanoparticles, water-soluble resveratrol can be obtained. A product prepared with the method of the invention is of high purity which is up to more than 98%, low production cost that is 20-30% lower than traditional methods, and high yield about 8-10% higher than traditional methods.; The method is also effective in increasing the solubility and oral bioavailability of resveratrol. According to the method of the invention, clean production and pollution-free purifying processes are realized, and the solvent therein is recyclable. 10. CN103156869-A

Title: Sanggenone C and sanggenone D extracted from morus plants and new medicine application of composition

This invention discloses sanggenone C and sanggenone D extracted from white mulberry root-barks, mulberry twigs and folium leaves and a new medicine application of a composition comprising the sanggenone C and the sanggenone D which are main ingredients, particularly the application of the single ingredient sanggenone C or sanggenone D and the composition composed of the sanggenone C, the sanggenone D, mulberrin, mulberroside, oxidized resveratrol, resveratrol and deoxynojirimycin in preparation of alpha-glucosidase inhibitor medicines. The composition is prepared by respectively extracting medicinal materials containing the chemical components ahove and blending according to certain proportions, or using several extraction methods for extracting the medicinal materials simultaneously containing the chemical components above. The white mulberry root-barks, the mulberry twigs and the folium leaves are accidentally found to contain the sanggenone C and the sanggenone D apart from containing alkaloids such as deoxynojirimycin; and the sanggenone C and the sanggenone D have stronger pharmacological activity than the alkaloids such as deoxynojirimycin.

11. CN102942455-A

Title: Method for extracting oxyresveratrol from mulberry branches

The invention relates to a method for extracting oxyresveratrol from mulberry branches. The mulberry branches are used as raw materials. The method includes: (1) the mulberry branches are extracted, concentrated and dried hy using an alcohol-water solution with 65%-75% alcohol volume content, and a crude extract is obtained; (2) degreasing treatment is performed on the crude extract by using petroleum ether, ethyl acetate is used for performing multiple extraction, ethyl acetate extract liquid is combined, a solvent is recovered through vacuum decompression, a fluid substance is obtained, the fluid substance is dried in vacuum at the temperature of 40 DEG C to obtain a semifinished product; and (3) the semi-finished product obtained in the step (2) is dissolved totally through an ethanol-water solution and mixed with macroporous resin D101, absorption is performed fully, a column is arranged, the ethanol-water solution is used as eluant to perform gradient elution or isocratic segmentation elution, the eluant is collected, concentrated and dried, and recrystailization is performed through a mixed solvent of acetone and petroleum ether to obtain oxyresveratrol crystals. The method can extract oxyresveratrol from the mulberry branches effectively, is simple to operate, low in cost and suitable for large-scale production. 12. US2004175788-A1

Title: Method for the separation of byproducts

This invention reported a method for the separation of at least one low molecular weight bioproduct from a cell culture mixture comprising uni-cellular organisms, broth and said at least one bioproduct by passing said cell culture mixture through a bed of an adsorb-ent material to adsorb said at least one bioproduct on said adsorbent material whereas said unicellular organisms and said broth are passing through said bed, whereafter the ad-sorbed bioproduct or bioproducts is/are eluted from said bed of adsorbent material, wherein said adsorbent material on its surface is provided with a material capable of preventing non-specific adsorption of said unicellular organisms to said adsorbent material,

13. US2012094361-A1

Title: Method of Separation of Algal Biomass from Aqueous or Marine Culture

This work reported a cross-flow membrane filtration methods for the removal or separation of algal cells from an aqueous environment. The methods of the invention may be used for the simultaneous algal harvesting/dewatering and water/wastewater purification and recycling.

14. Journal of the Medical Association of Thailand, 1989, 72, 71-73

Title: The optimum dose of Puag-Haad in the treatment of taeniasis

In this work forty-two per cent of 24 patients with Taeniasis saginata were cured by two-gram dose of a crude aqueous extract of the wood Artocarpus lakoocha, Puag-Haad, while eighty per cent of 25 patients were cured by three-gram dose which is comparable to the results of five-gram dose but had less side-effect. Thus, the three-gram dose of Puag-Haad is recommended in the treatment of taeniasis.

15. Southeast Asian J Trop Med Public Health, 1981, 12(4), 568-70

Title: Treatment of taeniasis with Puag-Haad: a crude extract of Artocarpus lakoocha wood

In this work, thirty-nine patients with tapeworm infection were treated with five grams of crude aqueous extract of Artocarpus lakoocha wood, "Puag-Haad". Seven of them vomited the drug immediately. Of the 32 patients, segments with scolices of Taenia saginata and of Taenia solium were recovered from 24 and 2 patients respectively. The side effects were vomiting and nausea,

16. J. Sci. Soc. Thailand, 1976, 2, 202-205

Title: A preliminary study on the antifungal activity of 2,4,3',5'-Tetrahydroxystilbene on Dermatophytes In this work, 2,4,3',5'-Tetrahydroxystilbene has been shown to inhibit the growth of Trichophyton nibum, Trichophyton mentagrophytes, Trichophyton tonsurans, Microsporum canis,. Microsporum gypseum and Epidermophyton floccosum. The minimal inhibitory concentration was 2.0 mM in all eases. It is inactive against Candida albicans.

Other related references:

1. J. of Planar Chromatography 24(2011)2,125-129

2. Chem. Pharm. Bull. 58 (11) 1492 (2010)

3. Brajilian journal of Pharmacology, 123, 1691 (1998)

4. Brain Research, 1017, 98 (2004)

5. Journal of Biological Chemistry, 277(18), 16340 (2002)

6. US Patent No. 20040175788A1 (2004)

7. US 20120094361A1 (2012)

8. J. Applied Polymer Science, 125(5), 3388-3898, (2012)

9. CN10159168Q-B, Method for extracting oxidized resveratrol

10. CN101591680-A ; CN1Q1591680-B

11. Chemistry & Biodiversity, 2006, 3, 1138-1143

12. Phytochemistry, 2012, 81, 42-49

13. Pak. J. Pharm. Sci., 2010, 23(4), 403-408

14. Journal of Natural Products, 2001, 64(11), 1457-1459

15. Medicinal Principles and Practice, 2009: 18: 223-227

Objectives of the invention

The main objective of the present invention is to provide a novel process for extraction and separation of oxyresveratrol from the heartwood extract of Artocarpus lakoocha Roxb.

Another objective of the process is to provide a greener approach for extraction of oxyresveratrol using water as an extractant. Also, for extraction of oxyresveratrol different common solvents such as alcohols, aqueous mineral acids and water are used for selecting the best solvent.

Yet another objective of the process is to provide a novel separation technique wherein membrane technology is used which is a less energy intensive, cost effective and eco-friendly process of separation. Summary of the invention

The present invention discloses a process for preparation of oxyresveratrol from heartwood of ARTOCARPUS LAKOOCHA ROXB., said process comprises of the steps of:

(i) Mixing of Artocarpus lakoocha Roxb. powder with water having concentration in the range of 0.64 mmolL ¹ to 0.76 mmolL ¹ by applying a temperature in the range of 30°C -70°C for 4 to 10 hours;

(ii) Subjecting the extract obtained from step (i) in a two compartment membrane cell containing nanofiltration membrane by stirring the aqueous phase of solution of the extract obtained in step (i) and circulating through the nanofiltration membrane by using a peristaltic pump at the flow rate range of 42.50 ml/min to 107.14 ml/min; and

(iii) Collecting the desired product at an interval of one hour from the permeate side of membrane.

Accordingly the present invention provides a process for extraction and separation of oxyresveratrol by using water as extracting solvent. In an embodiment of the invention, water is selected as the best extracting agent in comparison to the other solvents used. The other solvents used are aqueous mixture of mineral acids such as Hydrochloric acid and Sulphuric acid. In another embodiment of the invention methanol and ethanol are used as extracting solvent. In an embodiment of the invention, the effect of temperature on the extraction of oxyresveratrol is studied in the range of 30°C to 70°C and optimum temperature is found to be 50°C for extraction of oxyresveratrol using water as solvent. In another embodiment of the invention, the effect of time on the extraction of oxyresveratrol is studied in the range of 1 hr to 10 hrs and the optimum extraction time for extraction of oxyresveratrol using water as solvent is found to be 4 hours. The present invention relates to a process for separation of oxyresveratrol from the extractant using membrane technology. The membranes used for the separation process are alpha, beta and gamma cyclodextrin composite with polysulfone. In another embodiment of the invention, the water used for extraction is deionized water produced in Milli-Q system. In one embodiment of the invention the separation experiment is carried out in a disproportionate two-compartment membrane cell whose compartment volumes on the feed and permeate side are 150 and 120 ml respectively. The polymeric membrane is placed between the compartments with silicone -rubber packing and the cell is connected with a reservoir of 500 ml capacity. The solutions of the extracted compound in aqueous phase is stirred continuously and circulated through the membrane by using a peristaltic pump at the flow rate range of 42.50 ml/min to 107.14 ml/min. The area of the membrane is 19.6 cm ² and the trans membrane pressure of the experiment is in the range of 11.3 psi to 18.3 psi. The sample solutions are collected at an interval of one hour for seven hours from the permeate side and analyzed by UV and HPLC.

The present invention also relates to process for the extraction and separation of oxyresveratrol from heartwood of Artocarpus lakoocha Roxb. using water as extracting solvent and membrane technology as separation technique. The said process comprises the mixing of Artocarpus lakoocha Roxb. powder with warm water at 50°C for 4 hours, filtration of the powder through filter paper and filtrate is subjected to membrane treatment in a two compartment membrane cell containing beta-cyclodextrine-polysulfone composite nanofiltration membrane for separation of the product. The membrane can be reused for several cycles with consistent activity by backwashing the membrane with ethanol.The isolated oxyresveratrol shows strong antiglycation and antioxidant activities. From oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene) several derivatives including trans-2-methoxy- 4,3',5'-trihydroxystilbene, trans-2,3'-dimethoxy-4,5'-dihydroxystilbene, trans-4,3'-dimethoxy-2,5'- dihydroxystilbene, trans-2,4,3',5'-tetramethoxystilbene, cis-2,4,3',5'-tetramethoxystilbene, 2,4,3 ',5'- tetrahydroxybibenzyl and 2,4,3',5'-tetramethoxybibenzyl can be synthesized. Oxyresveratrol present in the Artocarus lakoocha Roxb. stem can be used as vermifuge for treatment of tape-worm infestation and the product has evergrowing interest of anti-aging, cardioprotective and anticancerous properties. The product has strong antioxidant activity because of the presence of flavonoids and phenolic compounds. The product has marked anthelmintic effect than standard drugs. The product has also neuroprotective effects against neurodegeneration in Alzheimer's disease.

Brief description of the drawings:

Figure 1: represents the Flow diagram of permeation experiment the whereas the parts are as following

1. Membrane cell

Magnetic stirrer

Magnetic capsule

4. Membrane

5. Feed tank

Peristaltic pump

N ₂ gas

8 Gas valve

9. Water vessel

10. Sample collecting valve

11. Pressure gauze

Figure 2: represents the Experimental set up of separation experiment

Detailed description of the invention:

The biological material i.e Artocarpus lakoocha roxb used for the process of the present invention is procured from Area: Kakojan P.O. Kakojan Pin code: 785107, Assam. The present invention relates to process for the extraction and separation of oxyresveratrol from heartwood of Artocarpus lakoocha Roxb. using water as extracting solvent and membrane technology as separation technique. In the process, water is selected as the best extracting agent in comparison to the other solvents used. The said process comprises the mixing of Artocarpus lakoocha Roxb. powder with warm water at 50°C for 4 hours, filtration of the powder through filter paper. For separation of oxyresveratrol from the extractant the filtrate is subjected to membrane treatment in a two compartment membrane cell containing beta-cyclodextrine-polysulfone composite nanofiltration membrane for separation of the product. The membrane is reusable for several cycles with consistent activity by backwashing with ethanol.

The membranes used for the separation process is beta cyclodextrin composite with polysulfone. The polysolfone-beta cyclodextrin (PS-CD) composite membranes are prepared indigenously using phase inversion technique. The separation experiment is carried out in a disproportionate two-compartment membrane cell whose compartment volumes on the feed side and permeate side are 150 and 120 ml respectively. The polymeric membrane is placed between the compartments with silicone -rubber packing and the cell is connected with a reservoir of 500 ml capacity. The solutions of the extracted compound of concentration in the range 0.64 mmolL ¹ to 0.76 mmolL ¹ in aqueous phase is stirred continuously and circulated through the membrane by using a peristaltic pump at the flow rate range of 42.50 ml/min to 107.14 ml/min. The area of the membrane is 19.6 cm ² and the trans membrane pressure of the experiment is in the range of 7.2 psi to 21.75 psi. The sample solutions are collected at an interval of one hour for seven hours from the permeate side and analyzed by UV and HPLC.

Examples

A.l. Extraction of oxyresveratrol (General Method)

10 gm of heart wood of Artocarpus lakoocha Roxb. is stirred continuously with different solvent such as water, methanol, ethanol, aqueous H ₂ S0 ₄ and aqueous HC1 at 30°C to 70°C for 10 hours. The samples are collected at a regular interval of time and filtered. The filtrate is analyzed for the presence of oxyresveratrol by UV visible spectrophotometer. The absorbance peak of oxyresveratrol is obtained at 329 nm. The concentration of oxyresveratrol is calculated using the calibration curve.

A.2. Optimization of the procedure:

From the extraction method given in A.1 following results have been obtained Table 1: Extraction of oxyresveratrol at different time in water

Ratio of wood and solvent : 1: 15

Temperature : 50°C

Table 2: Extraction of oxyresveratrol in different solvents and temperatures

Ratio of wood and solvent : 1: 15

Time : 4 hours

Table 3: Extraction of oxyresveratrol at different ratio of wood and solvent

Solvent: Water, Temperature: 50°C, Time: 4 hours

Table 4: Separation of oxyresveratrol by membrane

Concentration of feed = 0.76 mmoiL \ Pressure = 14.50 psi

Table 5: Separation of oxyresveratrol by membrane

Concentration of feed = 0.76 mmoiL \ Flow rate = 107.14 ml/min Membrane Pressure (psi) Flux % of recovery

(mmol m 'h ^_1 )

Alpha cyclodextrin- 7.20 5.81 63.18 polysulfone composite

14.50 8.81 88.86 NF membrane

21.75 9.02 90.69

Beta cyclodextrin- 7.20 5.81 64.43 polysulfone composite 14.50 9.88 98.07

NF membrane 21.75 7.65 55.78

Gama cyclodextrin- 7.20 3.24 35.30 polysulfone composite 14.50 5.01 50.10

NF membrane 21.75 4.23 42.25

Table 6: Separation of oxyresveratrol by membrane

Pressure = 14.50 psi, Flow rate = 107.14 ml/min

polysulfone composite 0.69 3.21 43.65

NF membrane 0.64 3.11 42.11

The Main Advantages of the Present Invention are :

1. The method is very simple and environmentally benign.

2. The product can be obtained in excellent yield upto 81% in case of extraction using water as solvent.

3. The product can be separated from the extracted mixture upto 98% using indigenously developed nanofiltration membrane.

4. Only the desired trans isomer is obtained and no cis isomerisation takes place during the extraction process.

5. The oxyresveratrol molecule is easily recoverable from the membrane cell by backwashing with ethanol under a pressure limit within the NF range.

6. The process is continuous and recyclable.

7. The membrane can be reused for consecutively more than ten times for separation of oxyresveratrol without any loss in activity of the membrane. Also, there is no generation of any toxic chemical waste to the environment making the process simple, green, environmentally benign, economically viable and less energy intensive.