Title:
PROCESS FOR HIGH EFFICIENCY REFOLDING OF RECOMBINANT PROTEINS
Document Type and Number:
WIPO Patent Application WO/2014/128726
Kind Code:
A3
Abstract:
A process of refolding a recombinant protein from inclusion bodies (IBs) formed inside a host cell, is provided. The process includes homogenising a wet cells slurry to obtain a cell lysate; incubating the cell lysate with a reducing agent to obtain a reduced cell lysate; isolating reduced IBs from the reduced cell lysate to obtain isolated IBs; solubilising the isolated IBs with a denaturing agent to obtain solubilised IBs; and subjecting the solubilised IBs to a unit process of refolding to obtain a refolded recombinant protein.
Inventors:
SONAR SANJAY (IN)
KRISHNAN ARCHANA (IN)
GHADE NIKHIL (IN)
SHAIKH FAIZA (IN)
KRISHNAN ARCHANA (IN)
GHADE NIKHIL (IN)
SHAIKH FAIZA (IN)
Application Number:
PCT/IN2014/000111
Publication Date:
January 08, 2015
Filing Date:
February 21, 2014
Export Citation:
Assignee:
BIOGENOMICS LTD (IN)
International Classes:
C12N15/00
Foreign References:
| US7186539B1 | 2007-03-06 |
Other References:
PALMER I ET AL: "Preparation and extraction of insoluble (Inclusion-body) proteins from Escherichia coli", CURRENT PROTOCOLS IN PROTEIN SCIENCE 2012 JOHN WILEY AND SONS INC. USA, vol. 1, no. SUPPL.70, November 2012 (2012-11-01), XP002731972, ISSN: 1934-3655
SINGH S M ET AL: "Solubilization and refolding of bacterial inclusion body proteins", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 99, no. 4, 1 April 2005 (2005-04-01), pages 303 - 310, XP027707167, ISSN: 1389-1723, [retrieved on 20050401]
VALLEJO LUIS FELIPE ET AL: "Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins", MICROBIAL CELL FACTORIES, vol. 3, 2004, XP002731981, ISSN: 1475-2859
SINGH S M ET AL: "Solubilization and refolding of bacterial inclusion body proteins", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 99, no. 4, 1 April 2005 (2005-04-01), pages 303 - 310, XP027707167, ISSN: 1389-1723, [retrieved on 20050401]
VALLEJO LUIS FELIPE ET AL: "Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins", MICROBIAL CELL FACTORIES, vol. 3, 2004, XP002731981, ISSN: 1475-2859
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