Title:
PROCESS FOR MODIFYING A CELL BY PUTTING MATERIAL INTO THE CELL
Document Type and Number:
WIPO Patent Application WO/2016/108527
Kind Code:
A1
Abstract:
The present invention relates to a process for modifying a cell by putting material into the cell. More particularly, the present invention is directed to a process for modifying a cell by putting material, such as protein, DNA, RNA, ribozyme, various compounds, collagen, cell nucleus, mitochondria or nanoparticle into the cell, by using a sealing-less device formed within one solid and comprises a first passage on which the cell passes; a second passage on which said material passes and connected to the first passage at a position randomly selected between both ends of the first passage; and an apparatus which applies pressure difference or electric potential difference on the first passage and the second passage.
Inventors:
LEE SANGHYUN (KR)
Application Number:
PCT/KR2015/014293
Publication Date:
July 07, 2016
Filing Date:
December 24, 2015
Export Citation:
Assignee:
FEMTOFAB CO LTD (KR)
International Classes:
C12M1/42; C12N1/20; C12N5/04; C12N5/07; C12N5/0735; C12N5/075; C12N5/076; C12N13/00; C12N15/87; G01N35/08
Domestic Patent References:
| WO2004092369A1 | 2004-10-28 |
Foreign References:
| JP2002516678A | 2002-06-11 | |||
| JP2005510236A | 2005-04-21 | |||
| JP2004248653A | 2004-09-09 |
Other References:
GENG, TAO ET AL.: "Transfection of cells using flow-through electroporation based on constant voltage", NATURE PROTOCOLS, vol. 6, no. 8, 2011, pages 1192 - 1208
Attorney, Agent or Firm:
SEO, Keunbok (507Nonhyeon-ro,Gangnam-gu, Seoul, KR)
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