Paclitaxel is a molecule of natural origin having wide spectrum antitumor activity, with the following structural formula: The compound, first recovered from Taxus brevifolia bark and from other natural sources, can be prepared semi-synthetically according to a number of procedures described in both scientific and patent literature.

US-4,924,011 discloses the semi-synthesis of paclitaxel using 10-deacetylbaccatine III protected at the C-7 hydroxyl with a trialkylsilyl group and subsequently acetylated at C-10. The resulting intermediate is reacted with (2R, 3S)-N-benzoyl-2-O-(l-ethoxyethyl)-3-phenyl-isoserine and the resulting product is deprotected to give paclitaxel.

WO-93/06094 discloses the preparation of paclitaxel by reacting a P-lactam precursor with 7-O-triethylsilyl-baccatine III, followed by mild acid hydrolysis.

According to US-5,476,954, paclitaxel is prepared starting from 10-deacetylbaccatine III esterified at C-7 with a 2,2,2-trichloroethoxycarbonyl group (TROC).

According to US-5,917,062 and US 6,020,507, the C-7 hydroxyl is protected with carbobenzoxy (CBZ) or with carbo-t-butoxy (Boc), followed by

selective acetylation of C-10 hydroxyl.

It is apparent from literature that a crucial aspect of paclitaxel semi- synthesis is to selectively protect the hydroxyls on the diterpen moiety (10-deacetylbaccatine III skeleton). The C-7 position is the most reactive and is therefore functionalized with groups which are easy to remove subsequently. The most commonly used group is triethylsilyl (TES), which is stable under the conditions used for the esterification of the other hydroxyls involved in the synthesis, and provides about 85% conversion yield.

Approximately 85% yields are obtained when an acetyl group is subsequently introduced at the C-10 position.

A novel process for the synthesis of paclitaxel has now been found, which provides higher final yields as well as other advantages compared with the known processes.

The process according to the invention comprises the following steps: a) protection of the hydroxyls at the 7-and 10-positions of 10-deacetylbaccatine III (10-DAB III), wherein R=R'=trichloroacetyl, or R'=acetyl and R is selected from t-butoxycarbonyl and trichloroacetyl, b) esterification of the hydroxyl at 13 with 3-phenyl-2-epoxypropionic acid

c) removal of the protective groups at the 7-and 10-positions (1) if they are both trichloroacetyl groups, followed by selective acetylation at the 10- position (2) and opening of the epoxide with sodium azide (3); or, alternatively, c') if R'= acetyl and R = trichloroacetyl, opening of the epoxide with sodium azide and simultaneous deprotection at the 7-position d) reduction of the azido group to amino group e) benzoylation to give the final product

The starting product is 10-deacetyl baccatine III (10-DAB III), which is extracted from the leaves of Taxus baccata. In the first step, 10-DAB III is quantitatively esterified at the C-7 and C-10 hydroxyls. When R=R'=trichloroacetyl, 10-DAB III is reacted with trichloroacetyl chloride in methylene chloride in the presence of triethylamine and of catalytic amounts

of 4-dimethylaminopyridine (DMAP). When R w R', first 10-DAB III is selectively acetylated with acetic anhydride in the presence of cerium, scandium, ytterbium salts, preferably CeC13.7H20. The resulting baccatine III is subsequently protected at C-7 with a t-butoxycarbonyl or trichloroacetyl group. The first can be introduced by reacting baccatine III with t-butoxy- pyrocarbonate in the presence of DMAP and ethyldiisopropylamine or, alternatively, following the procedure described in US 5,917,062. The trichloracetyl group can be introduced at position 7 by reaction with trichloroacetyl chloride in pyridine.

In the subsequent step (b), the hydroxyl at position 13 is esterified with 3-phenyl-2-epoxypropionic acid, preferably with its ammonium salt in toluene in the presence of dicyclohexylcarbodiimide, DMAP and p-toluenesulfonic acid, thereby obtaining (2R, 3R)-3-phenyl-2,3-epoxy-propionic acid baccatine III ester.

When both protective groups R and R'are trichloroacetyl, they can be removed using the conditions and reagents described by Zheng et al., Tetrahedron Lett., 1995,36,2001, and by Datta et al., J. Org. Chem., 1995, 60,761. Preferably, the two trichloroacetyl groups are removed with two equivalents of ammonium hydroxide. The deprotected compound is selectively acetylated at position 10 with acetic anhydride in the presence of cerium, scandium or ytterbium salts, preferably CeC13. 7H20.

The resulting compound is reacted with NaN3 in aqueous methanol in the presence of methyl formate, in the conditions reported in literature (Yamaguchi T., Tetrahedron Letters 39,5575-78,1998), to provide the corresponding azide.

Alternatively, when R = trichloroacetyl and R'= acetyl (d), the oxirane reacts with NaN3 to give the corresponding azide with deprotection at the 7- position, corresponding to the compound obtained at step (c').

The azide is reduced to amine in the subsequent step (d). The reduction can be carried out with hydrogen on catalyst or with PPh3. The product obtained at the last step (e) is benzoylated at the amino group to give paclitaxel. Benzoylation can be carried out with benzoic anhydride either simultaneously to reduction or subsequently on the isolated reduced product, using stoichiometric amounts of benzoyl chloride in the presence of potassium carbonate.

The following examples illustrate the invention in greater detail.

Example I-synthesis of 7-Trichloroacetyl-baccatine III In a 25 ml round-bottom flask, 0.603 g (1.03 mmol, 1.0 eq) of baccatine III were dissolved under magnetic stirring in 9.7 ml of dry pyridine at 25°C under nitrogen atmosphere. 138 ju. l (1.23 mmol, 1. 23 eq) of trichloroacetyl chloride were dropped into the clear pale yellow solution. 30 min after completion of the addition, a white precipitate formed. Further 120 p. l (1.07 mmol; 1 eq) of trichloroacetyl chloride were dropped into the reaction suspension, under the same conditions as above. After 20 min the solution had yellow-brown color. The almost complete conversion of the starting baccatine III was observed by TLC (Si02, n-hexane/EtOAc, 2: 3). The reaction mixture was diluted with CH2C12. The resulting solution was repeatedly washed with a CuS04 saturated solution, until pyridine had been completely removed (the solution had no longer blue color). The organic phase was concentrated under vacuum, dried over MgS04, filtered, and the solvent was evaporated, to obtain 0.612 g of a white-yellowish powder corresponding to 7-trichloroacetyl- baccatine III, having the following spectroscopic characteristics.

1H NMR (400 MHz, CDC13) : tippm= 1.08 (s, 3H, Me), 1.13 (s, 3H, Me), 1.86 (s, 3H, Me), 1.97 (ddd, 1H, J, = 14.4 Hz, J2= 10.3 Hz, J3= 1.9 Hz, C6- H), 2.13 (d, 3H, J=1, 2Hz, Me), 2.15 (s, 3H, Me), 2.30 (s, 3H, Me), 2.32-2.28 (m, 2H, C14-H2), 2.68 (ddd, 1H, J1= 14.4 Hz, J2= 9.3 Hz, J3= 7.3 Hz, C6-H),

4.04 (d, 1 H, J = 7. 0 Hz, C3-H), 4.17 (dd, 1H, Jl = 8. 4 Hz, J2= 1. 0 Hz, C20-H), 4.34 (d, 1H, J = 8.4 Hz, C20-H), 4.86 (t, 1H, J = 7.5 Hz, C13-H), 4.98 (dd, 1H, Jl= 9. 5 Hz, J2= 1.7 Hz, C5-H), 5.65 (d, 1H, J= 7.0 Hz, C2-H), 5.70 (dd, 1H, Jl = 10.4 Hz, J2= 7.4 Hz, C7-H), 6.42 (s, 1H, C10-H), 7.52-7.46 (m, 2H, arom), 7.62 (m, 1H, arom), 8.10 (m, 2H, arom); 13C NMR (100 MHz, CDC13) : bppm = 10.8,15.5,20.4,20.9,22.8,26.9,32.5,38.6,43.0,47.2,56.2,68.1, 74.5,75.5,76.5,77.0,79.0,80.5,83.7,89.9,129.0,129.4,130.3,13 2.0, 134.0,145.4,160.8,167.2,169.2,171.0,201.9.

Example 11-synthesis of (2'R, 3'R)-7-Trichloroacetyl-baccatine III- 13- (3'-phenyl-2', 3'-epoxypropionate) 0.164 g (1.00 mmol, 1 eq) of freshly prepared 3-phenyl-2- epoxypropionic acid were dissolved at 0°C in 30 ml of anhydrous toluene.

Subsequently 0.5 g (1 mmol, 0.68 eq) of 7- (trichloroacetyl)-baccatine III [7- (TCA)-baccatine III] were added under nitrogen atmosphere at 0°C. Finally, dicyclohexylcarbodiimide (DCC, 0.21 g, 1.00 mmol, 1.0 eq), 4-dimethylamino pyridine (DMAP, 0.084 g, 0. 68 mmol, 0.66 eq) and p-toluenesulfonic acid (p-TSA, 0.17 g, 0.10 mmol, 0.1 eq) were added, in succession. The solution was then heated at 70°C under magnetic stirring and nitrogen flow. The progress of the reaction was controlled by TLC (Si02, n-hexane/EtOAc, 3: 2).

The first spot, having Rf = 0.28, corresponds to 7- (TCA)-baccatine III epoxy ester. The second spot, having Rf= 0.11, corresponds to 7- (TCA)- baccatine III. After 3 hours, the mixture was cooled and the suspended solid was filtered. The precipitated dicylohexylurea (DCU) was washed with CH2C12.

The combined organic fractions were concentrated to dryness. The resulting crude (0.919 g) was chromatographed by flash chromatography (Si02, n- hexane/EtOAc, 3: 2). 0.100 g (0.14 mmol, 20 %) of unreacted 7-TCA- baccatine III and 0.435 g (0.49 mmol, 73 %) of (2'R, 3'R)-7-Trichloroacetyl- baccatine III-13- (3'-phenyl-2', 3'-epoxypropionate) having the following

spectroscopic characteristics were obtained: 'H NMR (400 MHz, CDC13) : bppm = 1.11 (bs, 6H, 2Me), 1.25 (bs, 1H, OH), 1.76 (d, 3H, J = 1.2 Hz, Me), 1.84 (s, 3H, Me), 2.02-1.92 (m, 3H, C14- H2 + C6-H), 2.13 (s, 3H, Me), 2.39 (s, 3H, Me), 2.69 (ddd, 1H, J, = 14.6 Hz, J2= 9. 3 Hz, J3= 7. 3 Hz, C6-H), 3.92 (d, 1H, J= 6. 9 Hz, C3-H) +, 3.97 (d, 1H, J = 4.7 Hz, C2'-H), 4.15 (dd, 1H, II = 8. 4 Hz, J2 = 1.0 Hz, C20-H), 4.31 (d, 1H, J = 8.3 Hz, C20-H), 4.33 (d, 1H, J= 4.7 Hz, C3'-H), 4.97 (dd, 1H, Jl = 9.5 Hz, J2 = 1. 8 Hz, C5-H), 5.63 (d, 1H, J = 6. 8 Hz, C2-H), 5.65 (dd, 1H, J, = 10.7 Hz, J2 = 7. 33 Hz, C7-H), 6.02 (dt, 1H, Jl = 8. 8 Hz, J2 = 1. 8 Hz, C13-H), 7.45- 7.30 (m, 5H, arom), 6.34 (s, 1H, C10-H), 7.49 (m, 2H, arom), 7.64 (m, 1H, arom), 8. 00 (m, 2H, arom); 13C NMR (100 MHz, CDC13) : 6ppm = 10.8,14.9, 20.8,21.0,22.5,26.5,32.4,35.7,43.2,46.7,56.0,56.1,57.9,70.9, 74.5,74.8, 76.4,76.7,79.0,80.6,83.6,89.8,126.8,128.7,128.9,129.2,129.3, 130.2, 132.6,133.0,134.1,141.3,160.7,166.3,167.1,169.1,170.1,201.3.

Example III-synthesis of (2'R, 3'R)-baccatine III-13- (3'-azido-2'- hydroxy-3'-phenyl-propionate.

In a 25 ml one-necked round-bottom flask equipped with magnetic stirrer, 0.397 g (0.45 mmol, 1 eq) of (2'R, 3'R)-7-trichloroacetyl-baccatine III 13- (3'-phenyl-2', 3'-epoxypropionate) were suspended at 25°C in 10.0 ml of CH30H. 1.26 ml of H2O, 1.26 ml of HCOOCH3 and 0.735 g (11.3 mmol, 25.0 eq) of sodium azide were added in succession. Temperature was raised to 50°C and the progress of the reaction was checked by TLC (SiO2, CHC13/EtOAc/MeOH, 12.0: 2.0: 0.3). Disappearance of the starting product and simultaneous formation of two products having Rf = 0. 22 and 0.29, respectively, were observed. The product having Rf = 0.29 was subsequently identified as the final product, whereas the product with Rf = 0.22 was (2'R, 3'R)-baccatine III-13- (3'-phenyl-2', 3'-epoxypropionate) formed as a reaction intermediate. The product having Rf = 0.29 growths in time to the

detriment of the product having Rf = 0.22. The reaction solution after 46 h had yellow brown color with a white precipitate (unreacted NaN3). The reaction was quenched after 46 h by addition of water, two further spots were observed, with Rf 0.38 and 0.13 (unrecovered decomposition products). The precipitated milky white solid was filtered, washed with water and then with AcOEt. A diphasic mixture was obtained, both phases being clear. The two phases were separated. The aqueous phase was extracted three times with AcOEt and the combined organic phases were concentrated and dried over MgS04. The mixture was filtered and the solvent was evaporated off, to obtain 0.335 g of a white-yellowish powder. The resulting crude was purified by flash chromatography (SiO2, CHCl3/EtOAc/MeOH 12: 2: 0.3), to obtain 0.279 g (0.36 mmol; 80 %; Rf=0. 22) of (2'R, 3'R)-baccatine III-13- (3'-azido-2'- hydroxy-3'-phenyl-propionate).

The compound has the following spectroscopic characteristics: 1H NMR (400 MHz, CDC13) : 8ppm= 1.14 (s, 3H, Me), 1.25 (bs, 4H, Me + OH), 1.67 (s, 3H, Me), 1.87 (ddd, 1H, J, = 13.9 Hz, J2= 11. 1 Hz, Je == 2.5 Hz, C6-H), 1.93 (d, 3H, J = 0.8 Hz, Me), 2.08 (d, 2H, J = 8.8 Hz, C 14-H2), 2.24 (s, 3H, Me), 2.26 (s, 3H, Me), 2.55 (m, 2H, C6-H + C7-OH), 3.28 (d, 1H, J= 8.4 Hz, C2'-OH), 3.77 (d, IH, J= 7.2 Hz, C3-H), 4.15 (dd, IH, J, = 8. 2 Hz, J2= 0.8 Hz, C20-H), 4.28 (d, 1H, J= 8.2 Hz, C20-H), 4.41 (m, 2H, C7-H + C2'-H), 4.93 (dd, 1H, Il = 9. 6 Hz, J2= 2. 0 Hz, C5-H), 4.96 (d, 1H, J = 4.4 Hz, C3'-H), 5.64 (d, 1H, J= 7.2 Hz, C2-H), 6.17 (dt, 1 H, J, = 7.9 Hz, J2= 1.2 Hz, C13-H), 6.30 (s, 1H, C10-H), 7.46-7.32 (m, 5H, arom), 7.46-7.32 (m, SH, arom), 7.50 (m, 2H, arom), 7.63 (m, 1H, arom), 8.06 (m, 2H, arom); 13C NMR (100 MHz, CDC13) : 8ppm= 9.8,15.3,21.1,21.9,22.6,27.0,35.6,35.8,43.3, 45.9,58.8,68.1,72.0,72.4,75.1,75.3,75.8,76.7,79.4,81.3,84.6, 127.9, 128. 9,129.2,129.5,130.3,133.4,134.1,135.3,142.2,167.2,170.5,171. 5, 203.8.

Example IV-synthesis of N-debenzoyl-paclitaxel In a 25 ml two-necked round-bottom flask, 0.102 g (0.13 mmol, 1.0 eq) of (2'R, 3'R)-7-hydroxy-baccatine III-13- (3'-azido-2'-hydroxy-3'-phenyl- propionate) were dissolved in 5.2 ml of freshly distilled CH2CI2 and the resulting pale yellow solution was added with H20 (0.05 ml), then with 0.071 g (0.26 mmol, 2.0 eq) of PPh3. The mixture was reacted at room temperature under magnetic stirring. After 16 h the reaction was checked by TLC (Si02, CHC13/CH30H 9: 1). The starting product (Rf=0. 61) had disappeared and a spot with Rf=0. 19 was observed. The reaction was quenched by diluting the mixture (of pale yellow color with white precipitate) with CHC13. Afterwards, the mixture was washed with distilled H20 and then with a sodium chloride saturated solution (brine). The bright yellow organic phase was dried over MgS04, then filtered and the solvent was evaporated off. 0.177 g of an ochre yellow oil were obtained. The crude was subjected to flash chromatography (Si02, CHC13/CH30H 9: 1), to obtain 0.074 mg (0.10 mmol; 76%) of N-debenzoyl-paclitaxel (pale yellow powder).

'H NMR (400 MHz, CDCl3) : 8ppm = 1.07 (s, 3H, Me), 1.09 (s, 3H, Me), 1.38-1.22 (bs, 2H, 20H), 1.75 (s, 3H, Me), 1.88 (s, 3H, Me), 1.90 (s, 3H, Me), 1.93 (s, 3H, Me), 2.20-1.96 (m, 6H, C14-H2 + C6-H, NH2 + OH), 2.52 (ddd, 1H, J1= 15.7 Hz, J2= 9. 5 Hz, J3= 5. 9 Hz, C6-H), 3.88 (d, 1H, J= 7. 2 Hz, C3- H), 4.10 (d, 1H, J = 4.0 Hz, C20-H), 4.17 (d, 1H, J = 4.0 Hz, C20-H), 4.22 (d, 1H, J = 8.0 Hz, C2'-H), 4.26 (d, 1H, J = 8.0 Hz, C3'-H), 4.56 (dd, 1H, Ji = 11.6 Hz, J2= 6.9 Hz, C7-H), 4.84 (d, 1H, J = 8.8 Hz, C5-H), 5.83 (d, 1H, J = 7.2 Hz, C2-H), 6.25 (t, 1H, J = 8.0 Hz, C13-H), 6.51 (s, 1H, C10-H), 7.20-7.00 (m, 8H, arom), 8. 13 (m, 2H, arom) ; 13C NMR (100 MHz, CDCl3) : bppm=9*8) 15.2,21.1,22.0,22.7,27.0,30.0,35.4,35.8,43.3,45.9,58.7,71.3, 72.3,75.2, 75.8,76.6,79.3,81.2,84.6,127.2,128.5,128.9,129.0,129.4,130.3 ,133.1, 134. 1,142.6,167.1,170.4,171.5,173.2,203.9.

Example V-synthesis of paclitaxel In a 10 ml round-bottom flask, 0.031 g (0.041 mmol, 1.0 eq) of N-debenzoyl-paclitaxel were dissolved in 1.25 ml of AcOEt. The clear yellow solution was added with 1.25 ml of a NaHC03 aqueous saturated solution. 7.1 ml (0.064 mmol, 1.5 eq) of benzoyl chloride were dropped into the resulting diphasic mixture, under strong magnetic stirring. The progress of the reaction was checked by TLC (Si02, CHC13/CH30H 9: 1). After disappearance of the starting product, a single spot having Rf=0.50 was observed. The reaction mixture was diluted with AcOEt. The organic phase was separated from the aqueous one, which was extracted with AcOEt (three extractions). The combined organic phases were dried over MgS04, filtered and concentrated.

The crude (0.037 g) was dissolved in a 1: 1 mixture of CH2CI2/ethyl ether, then n-pentane (0.030 g, 0.035 mmol, 86%) was added to precipitate paclitaxel, having the spectroscopic characteristics reported in literature.

Example VI-synthesis of the (2'R, 3'R)-7, 10-bis-trichloroacetyl-10- deacetyl-baccatine III-13- (3'-phenyl-2', 3'-epoxypropionate) In a 100 ml round-bottom flask 0.178 g (1.09 mmol, 1.0 eq) of freshly prepared 3-phenyl-2-epoxypropionic acid at 0°C were dissolved in 30 ml of anhydrous toluene. In the resulting solution, under nitrogen atmosphere and at 0°C, 0.663 g (0.79 mmol, 0.73 eq) of 7,10-bis- (trichloroacetyl)-10-deacetyl baccatine III [7,10-bis- (TCA)-IO-DAB III] were suspended. Finally dicyclohexylcarbodiimide (DCC, 0.224 g, 1.09 mmol, 1.0 eq), 4-dimethyl- aminopyridine (DMAP, 0.088 g, 0.72 mmol, 0.66 eq) and p-toluenesulfonic acid (p-TSA, 0.19 g, 0.11 mmol, 0.1 eq) were added, in succession. The reaction was carried out in heterogeneous phase at 70°C under magnetic stirring and nitrogen flow. The progress of the reaction was checked by TLC (Si02, n-hexane/EtOAc, 3: 2). The first spot having Rf = 0.28 corresponds to . 7, 10-bis- (TCA)-10-DAB III epoxy ester. The second spot having Rf = 0. 15

corresponds to 7,10-bis-(TCA)-10-DAB III. After 3 hours the mixture was cooled and the suspended solid was filtered. The dark yellow precipitate was washed with CH2C12 : the residual white solid was DCU. The combined organic fractions were concentrated and the resulting solid was subjected to flash chromatography (SiO2, n-hexane/EtOAc, 3: 2). 0.63 g of (2'R, 3'R)-7, 10-bis-trichloroacetyl-10-deacetyl-baccatine 111-13-3'-phenyl-2', 3'- epoxypropionate were obtained.

'H NMR (400 MHz, CDC13) : bppm=1. 12 (s, 3H, Me), 1.14 (s, 3H, Me), 1.76-1.60 (m, 2H, C6-H + OH), 1.81 (s, 3H, Me), 1.88 (s, 3H, Me), 2.04-1.98 (m, 2H, C14-H), 2.41 (s, 3H, Me), 2.69 (ddd, 1H, J, = 14.5 Hz, J2 = 9.3 Hz, J3 = 7. 3 Hz, C6-H), 3.89 (d, 1H, J= 7.2 Hz, C3-H), 3.98 (d, 1H, J= 4.0 Hz, C2'- H), 4.14 (d, 1H, J= 8.0 Hz, C20-H), 4.32 (d, 1H, J= 8. 0 Hz, C20-H), 4.34 (d, 1H, J= 4.0 Hz, C3'-H), 4.97 (d, 1H, J= 7.6 Hz, C5-H), 5.70-5.62 (m, 2H, C7- H + C2-H), 6.05 (dt, 1H, J= 8.4 Hz, J2 = 1. OHz, C13-H), 7.52-7.30 (m, 7H, ArH), 6.39 (s, 1H, C10-H), 7.45 (m, 1H, ArH) 7.99 (m, 2H, ArH); 13C NMR (100 MHz, CDC13) : 8pom = 10.9,15.1,20.8,22.6,26.3,32.5,35.6,43.1,46.7, 55.9,56.5,58.0,70.8,74.2,76.4,78.6,78.9,80.5,83.5,89.5,89.6, 126.8, 128.8,129.0,129.1,129.4,130.2,131.5,132.5,134.2,143.3,160.6, 161.1, 166.3,167.0,170.3,199.5.

Example VII-synthesis of (2'R, 3'R)-10-deacetyl-baccatine III-13- (3'-phenyl-2', 3'-epoxypropionate) In a 25 ml round-bottom flask, 0.174 g (0.18 mmol, 1.0 eq) of (2'R, 3'R)- 7,10-bis (TCA)-10-DAB III-13- (3'-phenyl-2', 3'-epoxypropionate) were suspended in 3 ml of CH30H. The resulting suspension was cooled to 0°C and 0.24 ml (0.36 mmol, 2.0 eq) of a 1.57 M NH3 aqueous solution were dropped therein, under strong magnetic stirring. The reaction was carried out for 15 min at 0°C, during which the suspension became yellow-greenish. After that, the mixture was warmed to room temperature and reacted for a further 5 min,

to completely dissolve the precipitate, obtaining a clear yellow-greenish solution. The complete disappearance of the starting compounds was checked by TLC (Si02, n-hexane/EtOAc, 3: 2), which gave a single spot on the baseline. The reaction mixture was diluted with H2O to obtain a milky white solution, the organic phase was extracted therefrom (3 extractions) with AcOEt (upon addition of the organic solvent, an emulsion formed which was broken by dissolving NaCI therein). The combined organic phases were dried over MgS04, filtered, and the solvent was evaporated. 0.194 g of white powder of (2'R, 3'R)-10-deacetyl-baccatine III-13- (3'-phenyl-2', 3'-epoxy- propionate) were obtained.

1H NMR (400 MHz, CDC13) : bppm=1@05 (s, 3H, Me), 1.09 (s, 3H, Me), 1.71 (s, 3H, Me), 1.72 (d, 3H, J= 1. 2 Hz, Me), 1.83 (m, 1H, C6-H), 1.95 (2H, d, J= 8. 8 Hz, C14-H2), 2.34 (s, 3H, Me), 2.58 (ddd, 1H, J1= 14. 6 Hz, J2= 9. 9 Hz, J3= 6.9 Hz, C6-H), 3.85 (d, 1H, J= 7. 3 Hz, C3-H), 3.95 (d, 1H, J= 4.4 Hz, C2'-H), 4.14 (d, 1H, J= 8.4 Hz, C20-H), 4.22 (dd, IH, Jl= 11. 3 Hz, J2= 6.6 Hz, C7-H), 4.27 (d, 1H, J= 8.4 Hz, C20-H), 4. 31 (d, 1H, J= 4. 4 Hz, C3'-H), 4.95 (d, 1H, J= 8. 8 Hz, C5-H), 5.16 (s, 1H, C10-H), 5.59 (d, 1H, J= 7. 3 Hz, C3-H), 5.99 (dt, 1H, (d, 1H, Jl= 8.8 Hz, J2= 1.2 Hz, C7-H), 7.30-7.50 (m, 7H, arom), 7.60-7.70 (m, 1H, arom), 7.90-8.00 (m, 2H, arom).

Example VIII-synthesis of (2'R, 3'R)-baccatine III-13- (3'-phenyl- 2', 3'-epoxypropionate) A solution of (2'R, 3'R)-10-deacetyl-baccatine III-13- (3'-phenyl-2', 3'- epoxypropionate) (138 mg) in 3 ml of dry tetrahydrofuran was added with 7.3 mg of CeCl3 7H2O and 0.073 ml of acetic anhydride. The reaction mixture was stirred at room temperature for 5 hours, during which time the reaction mixture became homogeneous. 1 g of ice was added, keeping under stirring for 1 hour. The organic solvent was evaporated off under vacuum and the residue was diluted with 5 ml of H2O. The formed precipitate was filtered and

dried under vacuum pump for 18 h. The resulting product (white powder, 130 mg) has the following characteristics: 1H NMR (400 MHz, CDC13) : Oppm=1. 05 (s, 3H, Me), 1.09 (s, 3H, Me), 1.71 (s, 3H, Me), 1.72 (d, 3H, J= 1. 2 Hz, Me), 1.83 (m, 1H, C6-H), 1.95 (2H, d, J= 8. 8 Hz, C14-H2), 2.34 (s, 3H, Me), 2.58 (ddd, 1H, Jl= 14.6 Hz, J2= 9. 9 Hz, J3= 6.9 Hz, C6-H), 3.85 (d, 1H, J= 7. 3 Hz, C3-H), 3.95 (d, 1H, J= 4.4 Hz, C2'-H), 4.14 (d, 1H, J= 8. 4 Hz, C20-H), 4.22 (dd, 1H, Jl= 11.3 Hz, J2= 6.6 Hz, C7-H), 4.27 (d, 1H, J= 8. 4 Hz, C20-H), 4. 31 (d, 1H, J= 4. 4 Hz, C3'-H), 4.95 (d, 1H, J= 8. 8 Hz, C5-H), 5.59 (d, 1H, J= 7. 3 Hz, C3-H), 5.65 (dd, 1H, Jl = 10.7 Hz, J2= 7.33 Hz, C7-H), 6.34 (s, 1H, C10-H), 7.30-7.50 (m, 7H, arom), 7.60-7.70 (m, 1H, arom), 7.90-8.00 (m, 2H, arom).

Example IX-synthesis of (2'R, 3'R)-baccatine III-13- (3'-azido-2'- hydroxy-3'-phenyl-propionate.

In a 25 ml one-necked round-bottom flask equipped with magnetic stirring, 0.17 g (0.45 mmol, 1 eq) of (2'R, 3'R) baccatine III 13- (3'-phenyl-2', 3'- epoxypropionate) were suspended at 25°C in 5 ml of CH30H. 0.63 ml of H2O, 0.23 ml of HCOOCH3 and 0.36 g (5.5 mmol, 12.5 eq) of sodium azide were added in succession. The mixture was heated to 50°C and the progress of the reaction was checked by TLC (Si02, CHC13/EtOAc/MeOH, 12.0: 2.0: 0.3). The reaction mixture after 46 h had yellow brown color with a white precipitate (unreacted NaN3). H20 (10 ml) was added and the precipitated milky white solid was filtered, washed with water and then with AcOEt. The two phases were separated, the aqueous phase was extracted three times with AcOEt and the combined organic phases were concentrated and dried over MgS04, filtered and the solvent was evaporated off, to obtain 0.20 g of a white- yellowish powder. The resulting crude was purified by flash chromatography (Si02, CHCl3/EtOAc/MeOH 12: 2: 0.3), to obtain 0.140 g of (2'R, 3'R)- baccatine III-13- (3'-azido-2'-hydroxy-3'-phenyl-propionate).

The compound has the same spectroscopic characteristics as the compound obtained in Example III.