Title:
PROCESS FOR PRODUCING A GENETICALLY MODIFIED SEED
Document Type and Number:
WIPO Patent Application WO/2020/209804
Kind Code:
A3
Abstract:
This invention relates to a process for producing a genetically modified seed. In particular, there is provided a process for producing a genetically modified Cannabis seed that germinates into a plant, the process comprising: (a) preparing a cell culture comprising genetically engineered Cannabis cells having at least one gene that expresses a psychoactive cannabinoid deleted; (b) establishing a callus culture for forming a somatic embryo; (c) forming a bio-ink comprising the somatic embryo that is encapsulated by a hydrogel and used as an artificial seed; and (d) three- dimensional (3D) printing the artificial seed comprised in the bio-ink. Also provided the 3D-printed artificial Cannabis seed having a shape other than that of a naturally occurring wild type Cannabis seed.
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Inventors:
LIM KAH MENG (SG)
Application Number:
PCT/SG2020/050227
Publication Date:
November 12, 2020
Filing Date:
April 13, 2020
Export Citation:
Assignee:
LIM KAH MENG (SG)
International Classes:
A01H4/00; B33Y10/00
Domestic Patent References:
| WO2016189384A1 | 2016-12-01 |
Other References:
LATA H. ET AL.: "Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. - an important medicinal plant", PHYSIOL MOL BIOL PLANTS, vol. 15, no. 1, 14 May 2009 (2009-05-14), pages 79 - 86, XP055644307, DOI: 10.1007/S12298-009-0008-8
HAIL RIHAN; FAKHRIYA KAREEM; MOHAMMED EL-MAHROUK; MICHAEL FULLER: "Artificial Seeds (Principle, Aspects and Applications)", AGRONOMY, vol. 7, no. 4, 3 November 2017 (2017-11-03), pages 1 - 15, XP055721649, DOI: 10.3390/AGRONOMY7040071
HUI HUI CHAI, YAO LU, CAN FANG, YA DAN LI, YUE JUN KANG, CHANG MING LI, LING YU: "3D-Printed seed planter and well array for high-throughput seed germination screening", INTEGRATIVE BIOLOGY, vol. 10, no. 1, 1 December 2017 (2017-12-01), pages 67 - 73, XP055760053, DOI: 10.1039/C71BO0178A
HAIL RIHAN; FAKHRIYA KAREEM; MOHAMMED EL-MAHROUK; MICHAEL FULLER: "Artificial Seeds (Principle, Aspects and Applications)", AGRONOMY, vol. 7, no. 4, 3 November 2017 (2017-11-03), pages 1 - 15, XP055721649, DOI: 10.3390/AGRONOMY7040071
HUI HUI CHAI, YAO LU, CAN FANG, YA DAN LI, YUE JUN KANG, CHANG MING LI, LING YU: "3D-Printed seed planter and well array for high-throughput seed germination screening", INTEGRATIVE BIOLOGY, vol. 10, no. 1, 1 December 2017 (2017-12-01), pages 67 - 73, XP055760053, DOI: 10.1039/C71BO0178A
Attorney, Agent or Firm:
AMICA LAW LLC (SG)
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